Biomedical subjects

S Takeuchi

Publications and source records attributed to S Takeuchi.

At least 361 records · Page 20Linked to original sources

PubMed1995

Thermosensitization of murine FM3A cells by transient incubation at low culture temperatures.

We show that when murine FM3A cells maintained at 37 degrees C were incubated at low culture temperatures between 24 degrees and 33 degrees C for 12-24 h, they became thermosensitive as compared to those incubated at 37 degrees C. The synthesis and accumulation of inducible HSP70 and constitutive HSC70 by heat shock decreased in cells incubated at 33 degrees C, whereas no such decreases were found in cells incubated at 37 degrees C. The accumulation of HSP105 because of heat shock also decreased in cells incubated at 33 degrees C. The decreased inducibility of HSP70, HSC70, and HSP105 by heat shock may in part be responsible for the increase seen in the thermosensitivity of the cells. Furthermore, the cellular concentration of HSP105 decreased in cells incubated at 33 degrees C but not in those incubated at 37 degrees C, whereas the concentration of HSC70 did not change at all in cells incubated at either temperature. The cellular content of HSP105, but not of HSC70, therefore may be a good indicator of the thermoresistant state of cells.

Animals↗

PubMed1995

Isolation of a new minor protein (Ovofactor-1), which has a cell growth promoting activity, from hen's egg white by heparin affinity chromatography.

A proteinaceous component eluted at the concentration of 0.8-1.0 M NaCl was obtained from hen egg white by heparin affinity chromatography. On SDS-PAGE, the component was resolved into three bands of 18.5, 16.5, and 16.0 kDa. The isoelectric point was estimated to be pH 8.8. This stimulated the DNA synthesis and proliferation of the cultured cells from chicken embryos. The amino acid composition and N-terminal analyses of the main 18.5 kDa component showed that this is a novel minor protein in egg white, and therefore was named "Ovofactor-1".

3T3 Cells↗

PubMed1995

Protein A in Staphylococcus aureus isolates from pigs.

The presence and quantity of protein A in Staphylococcus aureus 147 isolates from the tonsils of healthy pigs were examined by three methods. Cell-bound protein A was detected in 71 (48%), 104 (71%) and 123 (84%) of 147 isolates by the slide hemagglutination test, microplate hemagglutination test and enzyme-linked immunosorbent assay (ELISA), respectively. Extracellular protein A was not detected in any isolates by the microplate hemagglutination test. When the quantity of cell-bound protein A in the isolates was determined by the ELISA, most of the isolates contained about 0.8 to 2.2 micrograms of protein A/ml in bacterial cell suspensions of a concentration of MacFarland No. 3.

Animals↗

PubMed1995

Assay of proteases from Actinomyces pyogenes isolated from pigs and cows by zymography.

Extracellular proteases of Actinomyces pyogenes were assayed by zymography on SDS-polyacrylamide gel with the concentrated culture supernatant of 7 isolates from pigs and cows. When gelatin was used as a substrate, three proteases with molecular weights of 69, 59 and 55 kilodalton (kDA) were detected in all the isolates from both the pigs and cows. In addition to these proteases, 108 and 102 kDa proteases were detected in the swine and bovine isolates respectively. No protease bands appeared, however, when the calcium ion was absent from the buffer solution of the zymography. When casein was used as a substrate, the bands of 69, 59 and 55 kDa proteases were present, but 108 and 102kDa proteases were not detected in any of the isolates. The activity of all proteases was completely inhibited by phenylmethyl-sulfonyl fluoride and diisopropyl fluorophosphate but not by other protease inhibitors. From this result, it seems likely that the five proteases produced by A, pyogenes, originating from pigs and cows, are serine proteases.

Actinomyces↗

PubMed1995

Cultural differences in attitudes, values, and beliefs about osteoporosis in first and second generation Japanese-American women.

This study examines attitudinal differences related to osteoporosis between first and second generation Japanese-American women. In an interview, the women completed a battery of tests assessing their attitudes, values, and beliefs about the diagnosis, treatment, and follow-up care of osteoporosis. The groups differed in their general knowledge of osteoporosis, perceptions of the disease, attributions of its causes, anticipated and preferred support mechanisms for care, and anticipated areas of concern for self-or other-care. There were also considerable differences in treatment compliance and feelings toward physicians. The findings were discussed in relation to the effects of culture on health-care attitudes and behaviors.

Acculturation↗

PubMed1995

Angiogenesis in microvascular endothelial cells induced by glioma cells and inhibited by tumor necrosis factor in vitro.

Neovascularization is a prerequisite for glioma growth, so inhibition of angiogenesis may achieve control of glioma growth. We examined whether glioma cells induce angiogenesis and proliferation in microvascular endothelial cells from Fisher 344 rat brains by co-culture in a physical separation system with rat C6 glioma cells or rat T9 gliosarcoma cells. Endothelial cells cultured on type 1 collagen formed capillary-like structures. C6 glioma cells co-cultured with endothelial cells promoted the formation of these capillary-like structures. However, conditioned medium from C6 cells inhibited the proliferation of endothelial cells. T9 cells had little effect on the formation of capillary-like structures and no effect on the proliferation of endothelial cells. We also examined the effects of human tumor necrosis factor (TNF)-alpha on the formation of the capillary-like structures and on the proliferation of endothelial cells. Human TNF-alpha inhibited the formation of capillary-like structures induced by C6 glioma cells at a concentration of 100 U/ml, as well as the proliferation of endothelial cells at a concentration of 1000 U/ml. These results indicate that induction of angiogenesis varies with glioma cell lines and angiogenesis does not correspond with proliferation of endothelial cells. TNF-alpha can inhibit angiogenesis in gliomas in vitro.

Animals↗

PubMed1995

Cochlear blood flow and labyrinthine pressure: effects of glycerol on the inner ear.

Changes in cochlear blood circulation (CoBC) and perilymphatic pressure (Pp) after administration of physiological saline and 50% glycerol (12 ml/kg) were measured simultaneously in guinea pigs to examine the influence of glycerol on CoBC and Pp. The CoBC was measured by the laser-Doppler method. Although neither CoBC nor Pp changed after administration of physiological saline, glycerol produced a marked increase in CoBC and a slight decrease in Pp in 11 of 15 animals. However, the increase in CoBC was transient; CoBC reached a peak as soon as 30 min after glycerol administration and then returned rapidly to its initial value within 1 h after administration, although the Pp was still decreasing. Therefore, the response of CoBC to glycerol does not seem to be directly related to hyperosmolarity due to glycerol administration.

Animals↗

PubMed1995

Changes in blood biochemical parameters in tail-suspended rats.

There have been many studies on the effects of gravity on animals and humans. In particular, weightlessness and the time course of physiological shifts in space acclimation are the most significant problems in a space environment. In a laboratory setting, tail suspension in rats has been utilized for simulation of weightlessness. We therefore utilized this technique in order to study the influence of microgravity on blood constituents in rats. It was clarified that some blood biochemical parameters were effected for the duration of suspension exposure, and they are reported here.

Animals↗

PubMed1995

Alterations of the p16 (MTS1) gene in testicular, ovarian, and endometrial malignancies.

PURPOSE: The p16 (MTS1) gene codes for a cyclin-dependent kinase inhibitor and may be a new tumor suppressor gene. It is frequently mutated in a variety of cell lines established from tumors. This is the first report of screening for alteration of the p16 gene in testicular, ovarian and endometrial malignancies. MATERIALS AND METHODS: We examined alterations of p16 in 78 primary genital tumors (42 testicular, 21 ovarian and 15 endometrial cancers) and mononuclear cells from 2 patients with Lynch syndrome II as well as 5 testicular tumor cell lines by single-strand conformation polymorphism (SSCP) and Southern blot hybridization. RESULTS: The DNA from the p16 gene of 2 testicular tumors (5%), an ovarian cancer (4%) and a testicular tumor cell line (20%) had altered migration in gel electrophoresis as shown by SSCP. Analysis of DNA sequence of these samples revealed a polymorphism at codon 140. Southern blot hybridization detected neither deletions nor rearrangements of the p16 gene in any of the samples. CONCLUSIONS: Taken together, these results suggest that p16 alterations probably are not important for tumorigenesis of testicular, ovarian and endometrial tumors.

Base Sequence↗

PubMed1995

DNA aneuploidy in morphologically normal colons from patients with colon cancer.

DNA aneuploidy is common in colon carcinoma, colonic polyps, and ulcerative colitis. We found an interesting subset of patients with colon cancer. These individuals (14 of 230 cases, 6%) had hypodiploid aneuploidy in their morphologically normal-appearing colonic tissue. The aneuploid fractions were small, making up between 10 to 15% of the total events, and the ploidy pattern was not related to the ploidy pattern of the tumor. The clinical findings of the 14 patients were compared with those of patients who had diploid normal colons by age, location of the tumor, Dukes stage, and percent aneuploidy in the main tumor; the two groups were comparable. Both the normal colon and carcinomas of the 14 cases were studied by a newly developed "enriched" polymerase chain reaction for K-ras mutations. No K-ras mutations were found in the normal tissues, but mutations were found in the tumors of four cases. Cells from some colon cancers have a high degree of genetic instability, as shown by numerous mutations throughout the genome. Analysis of eight matched colon cancers and aneuploid, morphologically normal-appearing colons for genetic alterations, as measured by shifts in the electrophoretic mobility of microsatellite repeat fragments, showed that only one of eight colon cancer samples had microsatellite instability, which is the expected frequency. Taken together, the data suggest that individuals with colon cancer may have morphologically normal colonic tissue, which is genetically abnormal, and this abnormality may precede the development of mutations in K-ras.(ABSTRACT TRUNCATED AT 250 WORDS)

Adenocarcinoma↗

PubMed1995

[The clinical usefulness of urinary determinations of cytokeratin 19 fragment (CYFRA 21-1) in urothelial tumor].

The urinary CYFRA 21-1 value corrected for urinary creatinine (ng/ml/creatinine), was studied in the urine of patients with urothelial tumors. To examine its clinical significance we studied urinary CYFRA 21-1 excretion (ng/ml/creatinine), in a total of 22 urine samples from patients with bladder cancer, 7 from patients with renal pelvic and ureteral tumor, 6 from patients with urinary infection 6 from patients with urinary diversion called ileal conduit, and 8 from healthy adult men. The excretion of CYFRA 21-1 in urine was determined by two specific monoclonal antibodies (Ks 19.1 and BW 19.21). The mean value of urinary CYFRA 21-1 in healthy adult men was 1.96 +/- 1.33 (mean +/- SD) ng/ml/creatinine. Urinary CYFRA 21-1 showed a higher value in the urine of urinary infection and urinary diversion. As to bladder cancer, urinary CYFRA 21-1 showed a higher value in a larger volume of tumor than in a smaller volume of tumor in transitional cell carcinoma regardless of the grade and stage. These findings suggest that urinary CYFRA 21-1 may be a non-specific marker in urothelial tumors.

Adult↗

PubMed1995

[A case report of far advanced gastric cancer with multiple liver metastasis (H3) treated with transarterial intermittent chemotherapy and intradermal administration of low molecular lipopolysaccharide (LPSp) extracted from Pantoea agglomerans].

A case of far advanced gastric cancer with multiple liver metastasis (H3) was treated with transarterial intermittent chemotherapy (5-FU: 250 mg/week, Farmorubicin: 10 mg/4 weeks, MMC: 4 mg/2 weeks) and intradermal administration of low molecular lipopolysaccharide (LPSp) extracted from Pantoea agglomerans. The CT examination and endoscopy showed regression of the tumor and the patient was discharged from the hospital. LPSp was given at the concentration of 0.1 microgram initially, and the dose was gradually increased. Finally, the dose of LPSp was increased up to 70 micrograms. No serious side effect except fever was observed. The serum TNF-alpha levels were elevated and, histologically, CD 8(+) lymphocyte dominantly infiltrated around the tumor. These findings clearly indicated the immunological anticancer effect of LPSp. Intradermal administration of LPSp is a promising new adjuvant therapy to improve QOL without serious side effect.

Aged↗

PubMed1995

Mutations of p53 in Wilms' tumors.

Mutations of p53 frequently occur in a wide variety of cancers including lung, breast, gastrointestinal, brain, and hematologic malignancies. These alterations apparently contributed to development of the malignant phenotype. Wilms' tumor is one of the most common solid tumors in childhood. The frequency of p53 alterations in this tumor is unknown. We analyzed 66 Wilms' tumor samples for p53 mutations by single-stand conformational polymorphism (SSCP) following polymerase chain reaction (PCR). Samples with an abnormal SSCP pattern were reamplified and analyzed by direct sequencing method. Mutations of p53 were found in three (5%) of 66 Wilms' tumors within the coding region (exons 2-11), showing that the frequency of p53 mutations was low. Two mutations substituted amino acids residues and one encoded a stop codon. Two of the mutations were located in the mutational hotspots (exons 5 and 6); the other was in exon 10. These data suggest that p53 mutations are infrequent in the development of Wilms' tumors.

Base Sequence↗

PubMed1995

[A case of varicella pneumonia in an adult with moss-like substance in the bronchial mucosa].

A 33-year-old man complained of fever, cough and eruptions, and was admitted to our hospital. Chest X-ray films showed diffuse small nodular shadows. A moss-like substance in the bronchial mucosa was seen bronchoscopically. Transbronchial lung biopsy specimen showed infiltration of lymphocytes in the alveolar septa and peribronchiolar area. Transbronchial biopsy specimen of the bronchial lesion showed epithelial hyperplasia and necrosis. Immunohistochemical staining revealed VZV antigen.

Adult↗

PubMed1995

[Extracorporeal shock wave lithotripsy on patients with spinal cord injury with special reference to autonomic hyperreflexia].

We performed treatment of urolithiasis on 7 patients with spinal cord injury (6 males and one female, with a mean age of 41 years old) by extracorporeal shock wave lithotripsy (ESWL) using a Lithostar (Siemens). The level of injury was cervical in 3 patients and lower thoracic in 4. The treated stones were renal in 6 patients, including one staghorn caliculus, ureteral in 2, and bladder stones in 2 patients. ESWL was performed under general anesthesia in 2 patients, and in another patient, epidural anaesthesia was employed in the first several sessions, but thereafter treatment was safely continued with no anesthesia. The other 4 patients were treated without anesthesia. In most patients, the stones (9 stones in 6 cases) were easily disintegrated after treatment of from one to 14 sessions, except one case of staghorn caliculus, and the clearance of fragments was also satisfactory. In one patient, hypertension and bradycardia due to autonomic hyperreflexia were observed during ESWL, and the treatment was discontinued, but the stone was successfully disintegrated and the fragments were voided. In another patient, autonomic hyperreflexia was observed while the fragments passed through the ureter, although no signs of this reflexia were seen during the ESWL procedure.

Adolescent↗

PubMed1995

[A case of acquired von Willebrand disease due to pulmonary stenosis after Jatene's operation].

The case of a 9-year-old girl with pulmonary stenosis complicated by acquired bleeding tendency after Jatene's operation is reported. Coagulation study revealed that platelet count and von Willebrand factor were reduced. Catheterization study revealed severe pulmonary stenosis, the pressure gradient between the right ventricle (RV) and pulmonary artery (PA) being 190 mmHg. A link between pulmonary stenosis and bleeding tendency was suggested and the patient was diagnosed as having acquired von Willebrand disease due to activated platelet/von Willebrand factor interactions enhanced by "shear stress" at the site of pulmonary stenosis. Right ventricular outflow reconstruction was done when she was 9 years-old. Due to a protocol based on the results of the challenge test that we instituted to determine the efficacy and effective duration of blood derivatives and hemostatic agents, perioperative massive bleeding was avoided. Bleeding tendency disappeared and coagulation study findings normalized with correction of the abnormal hemodynamic state.

Child↗

PubMed1995

[Extracorporeal shock wave lithotripsy monotherapy using Lithostar--factors influencing actuarial residual stone rates].

For the evaluation of post treatment course after initial extracorporeal shock wave lithotripsy (ESWL) as a monotherapy using the Lithostar, the actuarial residual stone rate (ARSR) was calculated by the Kaplan-Meier formula in 713 calculi (255 renal and 458 ureteral calculi). ARSRs in all calculi at 3, 6, 12 and 48 months were 41.6, 30.5, 20.6 and 10.3%, respectively. Significantly higher ARSRs were observed in the following former group compared to the latter: large calculi to small or middle sized calculi, renal calculi to ureteral calculi (small or middle sized), and plural renal calculi (middle sized) to single renal calculi (middle sized). There was no significant difference either between large renal and large ureteral calculi, or overall single and plural calculi. These findings show that ARSRs calculated by the Kaplan-Meier formula clearly reveal factors influencing the ESWL effects.

Follow-Up Studies↗

PubMed1995

[Study on prevention of flare-up phenomenon following initial LH-RH analogue administration: combination therapy with diethylstilbestrol].

To eliminate the initial testosterone (T) surge and prevent the risk of flare-up induced by the first administration of luteinizing hormone-releasing hormone (LH-RH) analogue, we examined the effectiveness of short-term combination therapy with diethylstilbestrol (DES). Sixteen previously untreated patients with prostate carcinoma (Stage C: 4 cases, Stage D2: 12 cases) were randomly assigned to 4 groups. Each group included 4 patients. Group 1 received DES (500 mg daily injection) for 7 days, staring before the first subcutaneous injection of luteinizing hormone releasing hormone (LH-RH) analogue (3.6 mg of Zoladex depot); Group 2 received 500 mg of DES injection for 7 days prior to the initiation of therapy with the LH-RH analogue, and moreover received a DES injection for 7 days; Group 3 received 500 mg of DES injection for 7 days simultaneously with the first injection of the LH-RH analogue; Group 4 was pretreated with 500 mg of DES injection for 7 days prior to the initiation of therapy with the LH-RH analogue, with additional DES injection for 3 days. In groups 1, 2 and 4 the level of T decreased during the 7 days of DES therapy, and at 3 days after the first injection of LH-RH analogue increased again. The mean T value in groups 1, 2 and 4 decreased by 33.2 +/- 27.6% (mean +/- SE), 20.5 +/- 20.8%, 13.2 +/- 9.8%, respectively, at the day of the first injection of LH-RH analogue, and increased by 75.2 +/- 21.6%, 70.7 +/- 63.4%, 56.7 +/- 46.4%, respectively, at 3 days after the first injection of LH-RH analogue.(ABSTRACT TRUNCATED AT 250 WORDS)

Adenocarcinoma↗