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Biomedical subjects

S Takeichi

Publications and source records attributed to S Takeichi.

54 records · Page 3Linked to original sources
PubMed1990

Electroencephalographical analysis of acute paraquat poisoning.

The disturbances in the central nervous system of rabbits induced by gramoxon poisoning were studied by examination of subcortical electroencephalograms (EEGs) and power spectral analysis of EEGs. The following results were obtained. Administration of the fatal gramoxon (2.0 ml/kg i.p.) resulted in increases of slow frequency discharges from the reticular formation (RF), and from the hippocampus (Hpc) and sensorimotor cortex (Cm) after about 5 minutes and 20 minutes, respectively, detected by power-spectrum array. After about 30 to 40 minutes, the discharges from the Cm changed to rapid frequency discharges. The discharges from the Hpc and Cm decreased after about 50 to 60 minutes, and increased again in the agonal stage, and then seizure discharges developed. Soon after administration of gramoxon solution, the heart rate and respiratory rate increased and the PCO2, base excess (BE) and [HCO3-] decreased transiently. These findings indicate a shift to acute respiratory alkalosis and thereafter a shift to metabolic acidosis. Subsequently, the blood pressure, heart rate, respiratory rate, pH, BE and [HCO3-] decreased and the PCO2 increased. These findings indicate a shift to respiratory and metabolic acidosis. After about 70 minutes, the EEGs from all leads became almost flat, respiration ceased, and the blood pressure decreased to zero. After administration of gramoxon solution, the paraquat concentration in the blood increased rapidly. These results suggest that rapid increase in the paraquat concentration in the blood after gramoxon administration affects the central nervous system from an early stage.

Acid-Base Equilibrium↗
PubMed1989

Electroencephalographical analysis of acute drug intoxication--SS Bron solution-W.

Disturbances of the central nervous system due to abuse of SS Bron solution-W (B-solution), an antitussive and expectorant containing dihydrocodeine and other constituents, were studied in rabbits. Animals were given 20 ml/kg of B-solution orally at 4 hour intervals for 8 hours or more, and changes in the electroencephalograms (EEGs) from the reticular formation(RF), hippocampus(Hpc) and sensorimotor cortex(Cm) were examined. The following results were obtained. About 3 hours after administration of B-solution, the EEG from the Hpc showed low voltage, slow wave, the heart rate decreased rapidly, and the respiratory rate increased. After about 7 to 7.5 hours, the EEG from the Cm showed slow wave hypersynchrony and stimulation of the RF with square pulses of 2.0 V induced slight arousal reactions in the Cm and Hpc. The blood pressure and respiratory rate decreased, and ECG changes indicated atrial flutter-fibrillation and ventricular tachycardia. Power-spectrum array analysis indicated increase of slow frequency discharges in the EEGs from all regions. The slow wave hypersynchrony of the EEG from the Cm and slow wave, low voltage of the EEG from the Hpc seemed to be associated with hallucination and delusion.

Animals↗
PubMed1986

Acute toluene poisoning during painting.

Autopsy findings on a man who fell from a height due to acute toluene poisoning while painting are described. Gas chromatographic examination revealed that the toluene concentrations of his blood, lung, liver and brain were 48, 35, 65 and 80 micrograms/g, respectively. These toluene levels were not enough to be definitely lethal, but were high enough to anesthetize the central nervous system.

Accidental Falls↗
PubMed1986

Fluidity of cadaveric blood after sudden death: Part III. Acid-base balance and fibrinolysis.

In rats, fibrinolytic activity and acidosis increased rapidly after death. Postmortem fibrinolysis and the pH, base excess (BE), and [HCO3-] levels were affected by the method of sacrifice: the lower the pH, BE, and [HCO3-] levels, the higher the fibrinolytic activity. Conversely, in experiments using the vascular perfusion technique, low pH, BE, and [HCO3-] levels of the perfusate induced abundant release of plasminogen activator from the vascular wall.

Acid-Base Equilibrium↗
PubMed1986

Experimental studies on death by fire in automobiles and exhaust gas poisoning.

Studies were made on the acid-base balance, blood gases, and carbon monoxide (CO), cyanide, and sulfur dioxide concentrations in the blood of albino rabbits that died from automobile exhaust gas poisoning (group I) or fires in cars (complete combustion, group II; incomplete combustion, group III). In group I, the temperature and CO concentration increased gradually to 35 degrees C and 5.2% in 70 min. The animals died after 9 min, when the values were 20 degrees C and 5.2%, respectively. In group II the animals died after 9 min, when the values were 55 degrees C and 1.95%, respectively. In group III, the temperature was very high (870 degrees C), but the CO concentration was not (0.6-1.3%) after 4 min. The animals died after 5 min. In all experimental groups, marked acidosis and hypoxemia were seen, but the CO2 tension (PCO2) was high, in contrast to previous studies on pure CO poisoning. In group I, the level of carboxyhemoglobin (CO-Hb) was significantly higher (91.2 +/- 3.4% in arterial blood, 87.5 +/- 8.1% in venous blood; p less than 0.01) than in groups II and III. Although the O2 tensions of venous and arterial blood (PvO2, PaO2) were very low, that of arterial blood was higher, suggesting that O2 was still being utilized in the tissues at the time of death. In group II, CO-Hb was high (57.7 +/- 16.0% in arterial blood, 61.2 +/- 20.6% in venous blood) and the acid-base balance indicated marked acidosis. In group III, the CO-Hb, PCO2 and cyanide levels in the blood were very high.(ABSTRACT TRUNCATED AT 250 WORDS)

Accidents, Traffic↗
PubMed1985

Fluidity of cadaveric blood after sudden death: Part II. Mechanism of release of plasminogen activator from blood vessels.

Experiments by the vascular perfusion technique in isolated hind leg of the dog showed that after addition of various vasoactive drugs to the perfusate, fibrinolytic activity (plasminogen activator level) of the effluent was elevated. The effects of the drugs were completely inhibited only by the specific blocker of their receptor on the vascular wall. High K+ level of the perfusate also induced abundant release of plasminogen activator from the vascular wall.

Acetylcholine↗
PubMed1984

Mechanism of postmortem autolysis of skeletal muscle.

Male Wistar rats were treated with the carboxyl, thiol, and serine protease inhibitors, pepstatin, Ep-475[L-trans-epoxysuccinyl-leucylamide(3-methyl) butane; E-64-c], and chymostatin. Then the femoral muscles of these rats and control animals were used for preparation of myofibril proteins. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis was used to analyze the degradation of these myofibril proteins with time (day) after death. The protease activities of the muscle were also measured. Tropomyosin was degraded most rapidly, followed by the heavy chain of myosin, alpha-actinin, and light chains of myosin (L1 and L2). Actin and troponin-T were degraded slowly, still remaining unchanged 2 weeks after death. The degradation of protein was not inhibited by pepstatin but was inhibited strongly by Ep-475 and very strongly by chymostatin. Chymostatin inhibited degradation of all components except alpha-actinin more strongly than Ep-475. Data on enzyme activities were consistent with these findings. These results suggest that after death the components of myofibrils are degraded with various proteases at various rates depending on their properties or their structure and that the proteases involved in the degradation show some specificity.

Animals↗
PubMed1984

Fluidity of cadaveric blood after sudden death: Part I. Postmortem fibrinolysis and plasma catecholamine level.

In rats, fibrinolytic activity and plasma catecholamine levels increased rapidly after death. Postmortem fibrinolysis was affected by the method of sacrifice, but catecholamine level was not always affected. Immediately after death, the plasma adrenalin level was higher than the noradrenalin level, but then adrenalin remained unchanged or tended to decrease while noradrenalin tended to increase gradually with time. In human subjects after rapid death, the plasma catecholamine and fibrinolytic activity levels were high. However, in cases after slow death they were low.

Animals↗
PubMed1977

Studies on tumors produced by cells transformed with herpes simplex virus type 2.

Biological, histological, and ultrastructural studies were made on tumors produced in weanling hamsters inoculated with hamster embryo fibroblasts (HEF) transformed by herpes simplex virus type 2 or with cultured cells (tumor cell lines T1 and T2) derived from the tumor tissues. The malignancy of transformants increased through passages in vitro and in vivo. Histologically, two tumor cells lines (155-4T1 and U-15T1) produced fibrosarcomas and one (U-26T1) produced lesions resembling "malignant fibrous histiocytomas." Ultrastructurally, fibrosarcomas produced by 155-4T1 and U-15T1 consisted mainly of fibroblast-like cells with extracellular collagen fibers, whereas malignant fibrous histiocytoma-like lesions produced by U-26T1 consisted of undifferentiated cells, multinuclear giant cells, histiocyte-like cells, and fibroblast-like cells. Frequently, various kinds of "nuclear bodies" were found in the nuclei of tumor cells. Several herpes virus-like particles (120 approximately 140 nm in diameter) were detected in some nuclei of undifferentiated tumor cells produced by U-26T1.

Animals↗
PubMedDate not supplied

A case of homicidal poisoning involving several drugs.

Accidental or suicidal poisonings due to benzodiazepines have been previously reported. A case of fatal, homicidal poisoning with benzodiazepines, antipyretic analgesics (anti-inflammatory drugs), and beer is described here. In this homicidal poisoning, the drugs and beer were given to the decedent by his wife. Autopsy findings showed no clinically significant macroscopic findings except for slight postmortem change. Capillary gas chromatography coupled to mass spectrometry was employed to quantitate the drugs in biological fluids and stomach contents. Six drugs (brotizolam, triazolam, ibuprofen, dihydrocodeine, phenylpropanolamine, and chlorpheniramine) were identified and quantitated in blood, urine, and stomach contents. Although each drug was present in a very small quantity, the cause of death was determined to be the combination of these drugs and alcohol poisoning.

Adult↗
PubMedDate not supplied

A 20-kDa protein in human seminal plasma that is identical to gross cystic disease fluid protein 15 and prolactin-inducible protein.

A new method is described for isolating the 20-kDa protein, which used to be purified using an actin-immobilized column from human seminal plasma. This method employed ion-exchange column chromatography and isoelectric focusing separation. The level of seminal protein was determined by sandwich ELISA to be 1.06 +/- 0.27 mg/mL (mean +/- SD, n = 8). Its cDNA was cloned from a human salivary gland cDNA library by immunoscreening. The 553-nucleotide sequence included the 5' untranslated region and extended to the poly(A) tail. It encoded a protein of 118 amino acid residues in addition to a signal sequence of 28 residues. This sequence was identical to those of gross cystic disease fluid protein 15 and prolactin-inducible protein cDNAs. Northern blotting revealed the common expression in the submandibular gland and seminal vesicle. An immunohistochemical study in paraffin-embedded tissues from human male sex organs also indicated that the 20-kDa protein is mainly produced in the seminal vesicle.

Amino Acid Sequence↗