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Biomedical subjects

S Takagi

Publications and source records attributed to S Takagi.

766 records · Page 43Linked to original sources
PubMedDate not supplied

Cerebral hemodynamics in Shy-Drager syndrome: variability of cerebral blood flow dysautoregulation and the compensatory role of chemical control in dysautoregulation.

Cerebrovascular autoregulation and CO2 reactivity were measured repeatedly in 3 patients with the multiple system atrophy type of autonomic insufficiency (Shy-Drager syndrome) by means of the 133Xe injection method. The degree of cerebral blood flow (CBF) dysautoregulation showed day-to-day variations in 2 of the 3 patients. The CO2 reactivity was normal or supernormal in the supine position in patients with impaired autoregulation. In the head-up position the response to CO2 was slightly suppressed in 2 of the patients, suggesting that chemical control may have tended to compensate for CBF dysautoregulation. It is concluded that the mechanism of chemical control of the cerebrovasculature is different from that which controls autoregulation and may have partially compensated for CBF dysautoregulation.

Carbon Dioxide↗
PubMedDate not supplied

Presence of defensin in epithelial Langerhans cells adjacent to oral carcinomas and precancerous lesions.

We aimed to immunohistochemically study the localization of defensin (HNPs), a family of peptides with antimicrobial and cytotoxic activity, in oral tumor tissue. Therefore, tissue sections were embedded in paraffin, and defensin was immunostained by the streptavidin-biotin coupled peroxidase method. Langerhans cells were confirmed by indirect immunostaining with anti-S-100 protein polyclonal antibody. Melanocytes were stained with Fontana-Masson's stain. Neutrophils and intimal cells were stained by anti-defensin antibody. Langerhans cells in normal epithelium or dysplasic epithelium adjacent to squamous cell carcinoma and precancerous lesion were also stained. Defensins (HNPs) are nonspecific peptides that occur in neutrophils and protect against bacteria, fungi, and tumor cells. Since defensins are also found in epithelial Langerhans cells adjacent to tumor tissue, these peptides most likely have a role in tumor immunity.

Defensins↗
PubMedDate not supplied

Immunohistochemical staining of human alpha-defensin-1 (HNP-1), in the submandibular glands of patients with oral carcinomas.

The purpose of this study was the immunohistochemical localization and distribution of HNP-1 in the submandibular glands of patients with oral carcinomas. Tissue sections were embedded in paraffin, and HNP-1 was immunostained by the streptavidin-biotin coupled peroxidase method. Striated duct cells in the submandibular glands were stained with anti-defensin antibody. Neutrophils and capillary intimal cells were also stained. Defensins (HNPs) are peptides that occur in neutrophils and protect against bacteria and tumor cells. Human alpha-defensin-1 (HNP-1) is such a peptide, possessing both antimicrobial and cytotoxic activities. The presence of HNP-1 in striated duct cells in the submandibular glands of oral cancer patients, suggests a likely role in tumor immunity, for this peptide.

Capillaries↗
PubMedDate not supplied

Presence of human beta-defensin-2 in oral squamous cell carcinoma.

The purpose of this study was to demonstrate immunohistochemically the localization and distribution of human beta-defensin-2 (HBD-2), a peptide with antimicrobial activity, in oral carcinoma tissues. Tissue samples were embedded in paraffin, and HBD-2 was immunostained by the streptavidin-biotin coupled peroxidase method. Cancer cells in the cornified region of well differentiated squamous cell carcinomas were stained intensely. Stained cancer cells detected by anti-HBD-2 antibody were scattered among the cells of the non-cornified region.

Carcinoma, Squamous Cell↗
PubMedDate not supplied

Human alpha-and beta-defensin immunoreactivity in oral mucoepidermoid carcinomas.

The purpose of this study was to demonstrate the immunohistochemical localization and distribution of human alpha- and beta-defensins, peptides with antimicrobial activity, in oral mucoepidermoid carcinoma tissue. Tissue samples were embedded in paraffin and alpha- and beta-defensins were immunostained by the streptavidin-biotin coupled peroxidase method. Cancer cells that constituted the ducts, as well as neutrophils, were positively immunostained with the anti-alpha-defensin antibody (HNPs). On the other hand, epidermoid cells and intermediate cells were intensely stained with the anti-beta-defensin-2 (HBD-2) antibody. Mucous-secreting cells were clearly not immunostained with the anti-HBD-2 antibody. The epithelial hyperplasia region adjacent to the tumor tissues was also positively immunostained with the anti-HBD-2 antibody.

Carcinoma, Mucoepidermoid↗
PubMedDate not supplied

Dendritic cells in various human thyroid diseases.

The aim of this study is to determine the presence of S-100 protein-positive and CD1-positive dendritic cells (DCs) in tissues with various human thyroid diseases. Infiltrating DCs were frequently observed in papillary carcinoma and Hashimoto's disease, and these DCs were composed primarily of IDCs. The number of DCs was closely correlated with the expression of thyrocytes for S-100 protein and Ia-antigen. These findings suggest that DCs may play an important role in the immunologic defence mechanisms against papillary carcinomas and in the progression and self perpetuation of autoimmunity.

Antigens, CD↗
PubMedDate not supplied

Defensin-1, a peptide detected in the saliva of oral squamous cell carcinoma patients.

The saliva of patients with oral squamous cell carcinoma was subjected to reversed-phase HPLC, and a peak (P 14) was eluted with a linear gradient of acetonitrile. The molecular weight of P14 was 3,442 daltons by mass spectrometry. The results of amino acid sequencing by Edman degradation and homology studies indicated that P14 was consistent with a peptide, human alpha-defensin 1 (HNP-1) which belongs to the defensin family. The concentration of HNP-1 in the saliva of 6 squamous cell carcinoma patients before surgery and 11 after surgery was 12.3 +/- 8.6 and 6.5 +/- 5.9 micrograms/ml (mean +/- S.D.), respectively. In contrast, minimal amounts of HNP-1 were found in the saliva of adenocarcinoma patients and healthy volunteers. The concentration of salivary HNP-1 correlated positively with serum squamous cell carcinoma related antigen (SCC-Ag)level (r = 0.879, p < 0.01).

Adult↗