[Case of dysplasia epiphysialis hemimelica with reference to its differentiation from epiphyseal osteochondroma].
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Bone Diseases↗
Biomedical subjects
S Taguchi
Publications and source records attributed to S Taguchi.
[Pregnancy after hysterosalpingography, with reference to tubal pregnancy].
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Adult↗
Chronic low-dose treatment with enalapril induced cardiac regression of left ventricular hypertrophy.
Numerous studies suggest that the renin angiotensin system (RAS) is involved in the development of cardiac hypertrophy. In the present study we produced cardiac hypertrophy in rats subjected to abdominal aortic banding and also induced cardiac regression by the administration of an angiotensin converting enzyme (ACE) inhibitor, enalapril, at 3, 10 and 30 mg/kg/day. Each drug was administered to the rats for 6 weeks from 6 weeks after aortic banding. The left ventricular weight significantly decreased at 10 and 30 mg/kg/day of enalapril as well as the systolic blood pressure. Using the reverse transcriptase polymerase chain reaction, the increased levels of ACE and AT1 mRNA were significantly inhibited in the aortic banding rats treated with the above concentrations of enalapril. The ACE activity in both the plasma and heart tissue preparations was significantly inhibited by enalapril. Similar observations were also seen after the administration of angiotensin type 1 receptor blockade, E-4177, into the aortic banding rats. The treatment with enalapril at 3 mg/kg/day did not reduce the left ventricular weight or the systolic blood pressure in the aortic banding rats. However, this low-dose treatment did significantly decrease the left ventricle to body weight ratio in the aortic banding rats without a reduction of the systolic blood pressure. Therefore, using the low-dose enalapril, the ACE activity in plasma was in part inhibited and the levels of ACE mRNA also decreased in the heart tissue of aortic banding rats, while the level of AT1 mRNA showed no such decrease. These results thus indicate that chronic ACE inhibitor at low doses has a beneficial effect on the regression in the pressure-induced cardiac hypertrophy. It is thus assumed that this effect may also contribute to the presence of an alternate pathway for the conversion of angiotensin I to angiotensin II which might also act as a possible mechanism for cardiac regression.
Angiotensin Receptor Antagonists↗
Hyperamylasemia and S-type isozyme dominance in liver cirrhosis.
To investigate the mechanisms of serum amylase abnormalities in liver disease, we determined serum amylase levels, S-type isozyme proportion, clinical symptoms, and laboratory data in 38 cases of histologically confirmed liver cirrhosis and 19 controls. Of the 12 patients who were hyperamylasemic (12/38, 32%), 5 showed S-type isozyme dominance (5/12, 42%), whereas in the 26 normoamylasemic cirrhosis patients, only 5 were S-type isozyme dominant (5/26, 19%). Isozyme percentages were significantly higher (P less than 0.01) in the dominant-S-type cases than in the controls, and S-type dominance was found more frequently in the hyperamylasemic than in the normoamylasemic cirrhosis cases. Only ascites and esophageal varices were observed more frequently as clinical symptoms in the dominant-S-type cases. Our results suggest that amylase is not produced in the human liver, but that the decreased clearance rate of amylase, especially the S-type isozyme, may be a cause of hyperamylasemia and S-type isozyme dominance in cirrhosis.
Adult↗
Effect of exercise on age-related muscle atrophy.
Fifty-five-week-old male Wistar rats exercised voluntarily in running wheels for 10 weeks. The number and cross-sectional area of fibers in the slow-twitch soleus (SOL) and fast-twitch tibialis anterior (TA) muscles were recorded and compared with those of 20-week-old rats, and of age-matched rats not exercised. Muscle fibers were classified as fast-twitch oxidative glycolytic (FOG), fast-twitch glycolytic (FG), or slow-twitch oxidative (SO). After injection of horseradish peroxidase into the SOL and TA for retrograde neuronal labeling, oxidative enzyme activity of labeled motoneurons in the spinal cord was measured by microspectrophotometry. There were fewer FOG fibers in the SOL, and fewer FG fibers in the TA, at 65 weeks than at 20 weeks of age. The cross-sectional area of FOG and FG fibers in the TA was lower at 65 weeks than at 20 weeks of age. Exercise prevented the atrophy of FOG fibers in the TA. There were no age- or exercise-related differences in the number or oxidative enzyme activity of motoneurons in the SOL or the TA. These findings suggest that exercise can prevent the atrophy of FOG fibers by restoring their decreased metabolic capacity, and by inhibiting the degeneration of neuromuscular junctions.
Aging↗
Relationship between real-time monitoring of the graft motility and mucosal histology in swine intestinal transplantation.
We studied the correlation between the motility and the mucosal histology of the small bowel seeking to detect rejection in an early stage by real-time monitoring using a swine model. Intestinal transplantation (ITx) was performed orthotopically using FK506 immunosuppression. The distal about 20 cm segment of the allograft was exteriorized as a Thiry-Vella stoma for biopsies. Strain gauge (SG) force transducers were attached to the graft for real-time monitoring of graft motility. Pigs without ITx were used as controls (group 1). Rejection was classified into four groups by histologic findings: nonrejection (group 2), mild rejection (group 3), moderate rejection (group 4), and severe rejection (group 5). Migrating motor complex (MMC) phase III was analyzed for the following parameters: duration, amplitude, interval, motility index, velocity, and frequency of propagation. In group 2, all parameters were almost the same as those for group 1. In contrast, groups 4 and 5 showed most parameters significantly lower than those in group 1. In group 3, the contractility of the MMC was not significantly altered, but the frequency of the propagation was decreased significantly. In conclusion, graft motility detected by a real-time SG method correlated with the grade of mucosal histology. This method is useful to detect rejection at an early stage by examining the frequency of MMC propagation.
Animals↗
Serial evaluation of fatty acid metabolism in rats with myocardial infarction by pinhole SPECT.
BACKGROUND: Iodine 123-labeled 15-(p-iodophenyl)-3-(R,S)-methylpentadecanoic acid (BMIPP) is mainly trapped in the myocardium as triglyceride, depending on the adenosine triphosphate level. Ten percent to 20% of it is metabolized through alpha-oxidation after beta-oxidation; however, the precise mechanism of the regulatory pathways of BMIPP is yet to be clarified. METHODS AND RESULTS: A brief left coronary artery occlusion (10-30 minutes) was performed in 28 male Wistar-Kyoto rats. Dual single photon emission computed tomography images of BMIPP and thallium 201 were obtained 3 days and 24 days after the operation. The activities of 3-hydroxyacyl-coenzyme A dehydrogenase (HAD), citrate synthase (CS), and alpha-glycerol-phosphate dehydrogenase (GPD) were then measured in both ischemic and nonischemic regions. BMIPP and Tl-201 chloride severity scores were also evaluated conventionally. CS and HAD levels were significantly lower in the ischemic region than in the nonischemic region in the chronic group (CS, 102.9 +/- 28.1 vs 138.7 +/- 33.7 micromol/g/min, respectively, P =.0051; HAD, 54.7 +/- 20.1 vs 78.6 +/- 18.7 micromol/g/min, respectively, P =.0031). There was no difference in GPD between the ischemic and nonischemic regions. The BMIPP severity score had closer inverse relations with HAD (acute, r = -0.82; chronic, r = -0.80) and CS (acute, r = -0.87; chronic, r = -0.81), but not with GPD, than did Tl-201 chloride severity score. CONCLUSIONS: BMIPP imaging correlates well with the activities of HAD and CS, suggesting that a decrease in BMIPP uptake reflects deterioration of both fatty acid metabolism and citrate cycle and shows information other than regional myocardial perfusion.
3-Hydroxyacyl CoA Dehydrogenases↗
A single skeletal muscle powered ventricle that assists both systemic and pulmonary circulations.
Extraaortic counterpulsation using skeletal muscle powered ventricles (SMPVs) has been studied mainly for the purpose of assisting the systemic circulation. The purpose of this study was to investigate the possibility of assisting both the systemic and pulmonary circulations using a single SMPV. An SMPV that counterpulses the systemic circulation is placed beside the right atrium (RA) or the right ventricle (RV), the former after procedures such as a Fontan operation, and the latter in cases of biventricular failure. Because the SMPV dilates passively during cardiac systole, it is designed to press the RA or the RV directly from outside. This study is a preliminary report, and only the ability of a specially constructed SMPV to dilate was assessed. Using seven dogs with heart failure, the SMPV dilated with an internal pressure of 88 mmHg and 1.0 cm, while the volume inside increased by 10.8 ml. From these results, it is suggested that the SMPV has enough dilating power to press the RA or to assist the RV while counterpulsing the systemic circulation.
Animals↗
Application of membrane filters for spectrophotometric determination of cationic surfactants in river water and sediment.
Cationic surfactant (CS+) in urban river water and sediment was extracted and determined spectrophotometrically with 2 membrane filters. The CS+ in the water samples, mostly in the form of an ion associate with the coexisting anionic surfactant (AS), was collected on a polytetrafluoroethylene (PTFE) membrane filter and eluted with methanol. Bromphenol blue (BPB), hydrochloric acid, and water were added to the methanol solution successively, and the mixed solution was filtered through a mixed cellulose ester membrane filter. The CS+-BPB- ion associate, formed by a counter ion exchange, was collected on the filter and dissolved into N,N-dimethylformamide (DMF) together with the mixed cellulose ester membrane filter. After addition of 2 drops of triethanolamine, the absorbance of the DMF solution was measured. The CS+ in sediment samples was extracted with methanol by ultrasonic irradiation; the methanol solution was then passed through a PTFE membrane filter and evaporated to dryness. The CS+ was redissolved in a small amount of methanol. For water samples, recoveries and relative standard deviations for 0.30 microM benzyldimethyl-tetradecylammonium ion, a standard material, were > or =93 and < or =5%, with a detection limit of 0.02 microM. Concentrations of CS+ in sediments were much higher than those in water samples, indicating that CS+ is adsorbed on the surface of the sediment.
Adsorption↗
Effects of nonpulsatile and pulsatile right ventricular bypass on pulmonary circulation.
Effects of nonpulsatile flow on pulmonary circulation was examined experimentally. Tendency to accumulate extravascular lung water as compared with pulsatile flow was of particular interest.
Animals↗
Effect of the type of serum in the medium on sodium ascorbate-induced cytotoxicity.
Sodium ascorbate induced cytotoxicity against human glioblastoma T98G cells in RPMI1640 medium supplemented with fetal bovine serum or human serum samples was studied. Several human serum samples significantly reduced the cytotoxic activity of sodium ascorbate, regardless of sex, age or the disease of the serum donor with or without heat-inactivation of the serum. ESR spectroscopy revealed that this serum effect was not simply due to the alteration of the ascorbyl radical intensity, produced from sodium ascorbate. The present study suggests that the apoptosis-inducing activity of sodium ascorbate might be significantly affected by human serum.
Adult↗
Inhibition of cytotoxic activity of ascorbate by human cancer patient sera.
Cytotoxic activity of sodium ascorbate against a human glioblastoma T98G cell line was concentration-dependently inhibited by serum in the RPMI1640 medium. The inhibitory effect of sera from pancreatic or stomach cancer patients was significantly higher than that of fetal bovine serum (FBS), with or without heat-inactivation. The cytotoxic activity of sodium ascorbate almost completely disappeared in 60-80% of patient sera. ESR spectroscopy revealed that both patient sera and FBS increased the ascorbyl radical intensity, but to significantly lower extents, as compared with that attained by RPMI1640 medium. The present study suggests the importance of re-evaluating the efficacy of not only ascorbate but also other chemotherapeutic drugs under more physiological conditions.
Aged↗