Pro-UK delta GS1 was designed as a long-life and thrombin-resistant derivative of pro-urokinase (pro-UK) by deleting the growth factor domain of pro-UK and introducing a glycosylation site near the thrombin cleaving site for thrombin-resistance using site-directed mutagenesis. An expression plasmid for pro-UKDGS1, pIH1UK delta GS1SEd1-5 was constructed and introduced into Namalwa KJM-1, a lymphoblastoid cell line adapted to serum-free medium, and cells resistant to G418 and Methotrexate (MTX) were obtained. Amongst them, the highest pro-UK delta GS1 producer (resistant to 200 nM of MTX), clone 2-9, was selected and used for further studies. Under the conventional conditions, i.e. at 37 degrees C in serum-free ITPSGF medium (based on RPMI-1640 medium), the oligosaccharide structure of pro-UK delta GS1 produced by clone 2-9 mainly consisted of fucose (Fuc)-containing biantennary complex-type oligosaccharide. Addition of dexamethasone (Dex), changed the carbohydrate contents in the media, and a shift down of incubation temperature caused a change in oligosaccharide structure of pro-UK delta GS1 from mainly Fuc-containing biantennary to mainly Fuc-containing tri- and tetraantennary complex-type oligosaccharide. The modulated pro-UK delta GS1 showed superior in vivo activity for a canine femoral thrombosis formed by inserting a copper-coil.

Amino Acid Sequence↗

PubMedDate not supplied

Weak association between retinopathy of prematurity and neurological disorders in childhood.

An attempt was made to elucidate the association between retinopathy of prematurity (ROP) and neurological disorders in infants of very low birth weight. In this retrospective study, 1081 Japanese children weighing less than 1500 g at birth were classified into four subgroups according to birth weight. In each subgroup, the association among cerebral palsy (CP) or mental retardation (MR) and ROP, treated ROP, and cicatricial ROP was analyzed statistically. In the population as a whole, CP and MR were positively associated with ROP. However, in the birth weight subgroup analysis, no significant association was found. We can conclude that there is a weak association between ROP and CP or MR.

Cerebral Palsy↗

PubMedDate not supplied

Wegener's granulomatosis (WG) and insulin dependent diabetes mellitus (IDDM).

We report a case of a 14 year-old girl with Wegener's granulomatosis (WG) complicated by its direct brain invasion and insulin dependent diabetes mellitus (IDDM). This case was diagnosed as WG early in its onset and successfully treated. As for IDDM of this case, the presence of HLA-DR4 and the islet cell surface antibody (ICSA) was demonstrated. To our knowledge, this is the first case report on IDDM associated with WG. We consider that WG may precipitate the development of IDDM in a genetically predisposed subject.

Adolescent↗

PubMedDate not supplied

[Proceedings: 337. Relationship of brain evoked potentials between classical conditioning and operant conditioning (author's transl)].

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Animals↗