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Biomedical subjects

S Suehiro

Publications and source records attributed to S Suehiro.

At least 127 records · Page 7Linked to original sources
PubMed1987

Amplification of human immuno-deficiency virus production from a virus-producing cell line in culture at high cell density.

The amount of infectious virus released into the culture fluid from human immunodeficiency virus (HIV)-producing MOLT-4 cells (MOLT-4/HIV HTLV-III) was measured as a function of the culture conditions and cell density. After 3 days, the daily yield of infectious virus per cell in the fluid of non-growing cells cultured at high cell density was about 15 times higher than the accumulated yield from growing cells cultured at low cell density. SDS-PAGE analysis also showed a similar difference in the amounts of major gag protein p24.

Cell Line↗
PubMed1987

Suppression by neurotropin of production of human immuno-deficiency virus by the virus-producing MOLT-4 cell line.

The effect of neurotropin on human immuno-deficiency virus (HIV) production in a virus-producing MOLT-4 (MOLT-4/HTLV-III) cell line was studied. Culture of the cells for 4 days in the presence of 100 micrograms/ml of neurotropin resulted in a 72% decrease in the number of infectious virus particles released into the culture fluid. A pulse-labeling experiment to analyze the synthesis of HIV proteins suggested that neurotropin may affect the budding process of the virus.

Acquired Immunodeficiency Syndrome↗
PubMed1986

Carcinoma of the stomach in atomic bomb survivors. A comparison of clinicopathologic features to the general population.

The results of surgical treatment of gastric cancer were reviewed retrospectively for 135 atomic bomb survivors and 377 control patients. The mean age was significantly higher in the survivors than in the controls. Otherwise, both groups were quite comparative especially in terms of the stage of the disease. Histopathologically, the rates of poorly differentiated or undifferentiated types of carcinoma and secondary lymph node involvements were significantly lower in the survivors than in the controls. There were no significant difference between the two groups in postoperative morbidity and mortality rates and long-term survival rate. The incidence of second primary malignancies, however, was apparently higher in the survivors than in the controls.

Aged↗
PubMed1986

[Comparative study of estrogen receptor assays between immunocytochemical assay using monoclonal antibody and the dextran- coated charcoal method].

Estrogen receptor (ER) was measured by the estrogen receptor-immunocytochemical assay (ER-ICA) using monoclonal antibody in 36 mammary carcinomas. Simultaneously, ER and progesterone receptor (PgR) were measured by the dextran coated charcoal (DCC) method. In ER-ICA, tumor cells with estrogen receptors were histologically observed. The proportion of ER-positive tumor cells among tumor tissues and the levels of staining intensity were also examined. Results were compared to those obtained by the dextran coated charcoal (DCC) method. Tumors were evaluated as ER-ICA-positive when they contained more than 10% ER-positive cells. ER at a level of more than 5.0 fmol/mg protein was evaluated ER-positive and PgR at more than 5.0 fmol/mg protein as PgR-positive by the DCC method. Twenty-one out of 24 ER-ICA-positive tumors were ER-positive by the DCC method, and 11 out of 12 ER-ICA-negative tumors were ER-negative by the DCC method. Correlation of ER-ICA and ER by the DCC method was 88.9%. There were 11 PgR-positive tumors, and all were included among ER-ICA-positive tumors which had a high proportion of ER-positive cells and high staining intensities.

Antibodies, Monoclonal↗
PubMed1986

[Fibrocystic disease of the breast in autopsy cases with special reference to the age frequency distribution of epithelial hyperplasia and its relation to liver cirrhosis].

A histological examination of 306 breast tissues taken consecutively from autopsied Japanese women aged from 12 to 104 during the period between 1973 and 1984, excluding breast cancer cases, was undertaken. Two peaks at the ages of 40 to 44 and 55 to 59 were seen in the age-frequency distribution of fibrocystic disease (FCD), blunt duct adenosis (BDA), and duct papillomatosis (DP). There was no significant difference in the frequency of FCD, BDA, and apocrine metaplasia between the cases of liver cirrhosis and those without any hepatic disorders (controls). On the other hand, DP was seen more frequently and in higher degree in those with liver cirrhosis as compared with controls.

Adult↗
PubMed1985

Purification and some properties of mitochondrial glutamate:glyoxylate aminotransferase and mechanism of its involvement in glycolate pathway in Euglena gracilis z.

Euglena contains glutamate:glyoxylate aminotransferase (GGT) both in mitochondria and in cytosol. Both isoforms were separated from each other by DEAE-cellulose chromatography. The mitochondrial enzyme had an apparent Km of 1.9 mM for glutamate and the cytosolic enzyme 52.6 mM. Mitochondrial GGT was further purified by ammonium sulfate fractionation, isoelectric focusing, and gel chromatography. It had a molecular weight of 141,000 and an isoelectric point of pH 4.88; the optimum pH was 8.5. Its apparent Km values for glutamate and for glyoxylate were 2.0 and 0.25 mM, respectively. In addition to glutamate, mitochondrial GGT used 5-hydroxytryptophan, tryptophan, and cysteine as amino donors in the transamination to glyoxylate. Alanine did not support the activity. The relative activity of the enzyme for amino acceptors on the transamination from glutamate was 4-hydroxyphenylpyruvate greater than phenylpyruvate greater than glyoxylate greater than hydroxypyruvate. Pyruvate and 2-oxoglutarate were not used in the reaction. Evidence that GGT functions mainly in the irreversible transamination between glutamate and glyoxylate is presented. The functional significance of GGT in the glycolate pathway of Euglena is also discussed.

Animals↗
PubMed1984

Early gastric cancer with widespread lymph node metastases; a case report.

The resected stomach from a 58-year old male patient showed a 4.3 X 3.2 cm, depressed (II c) lesion in the body, histology of which revealed moderately differentiated tubular adenocarcinoma with invasion reaching the submucosa. Widespread metastases of lymph nodes including superior mesenteric and para-aortic ones were noted. The patient once worked in a mustard gas (Yperite) factory, and had chronic bronchitis. The exposure to this toxic substance was discussed in relation to the diffuse metastases of cancer.

Adenocarcinoma↗
PubMed1984

Tolerance of the cirrhotic liver to normothermic ischemia. A clinical study of 15 patients.

Temporary occlusion of hepatic inflow, namely, the Pringle maneuver, was adopted for 15 patients with liver cirrhosis who underwent partial hepatectomy. The warm ischemia time ranged from 2 to 32 minutes with an average of 19 minutes. The procedure did not cause any harmful effects on systemic hemodynamic and postoperative liver function. The results in our patients were compared with those of 15 comparable control patients who had been operated on over the same period of time without inflow obstruction. The Pringle maneuver significantly diminished the estimated blood loss during surgery, intraoperative and postoperative complications, and suppressed the operative mortality rate from 20 percent to 0.

Adult↗
PubMed1984

The negative effect of splenectomy on the prognosis of gastric cancer.

During the 11 year period from 1970 through 1980, 103 patients underwent total gastrectomy for gastric cancer. Splenectomy was performed in 53 of the patients because of perioperative accidental injury or an operative finding of tumor metastasis to the lymph nodes or direct invasion to the splenic hilus. Retrospective comparisons were carried out among the 53 splenectomized and 50 nonsplenectomized patients in terms of the long-term survival rate and early and late complications. The two groups of patients were quite similar with regard to rates of curative resection and stages of disease. The 5 year cumulative survival rates overall and in the splenectomized and nonsplenectomized groups were 23.1, 21.8 and 23.8 percent, respectively. Splenectomy did not affect the prognosis of gastric cancer. In addition, there were no differences between the two groups in the incidences of early surgical complications and infectious disease of late onset. Our results suggest that, in the treatment of gastric cancer, splenectomy may not be avoided, either when the spleen is injured perioperatively or when cancer involvement is suspected in the splenic hilus.

Combined Modality Therapy↗
PubMed1984

Intraoperative ultrasonography in the surgical treatment of hepatic tumors.

Intraoperative ultrasonography was performed in 50 patients with various liver tumors; 35 hepatocellular carcinoma (hepatoma), 2 cholangiocellular carcinoma, 5 secondary liver cancer, 5 cavernous hemangioma, and 3 hyperplastic nodule in the cirrhotic liver. Various kinds of liver surgery were carried out on these patients; subsegmental resection of the liver in 19, segmentectomy in 12, hepatic lobectomy in 10, tumor extirpation in 4, and palliative surgery in 5 instances. Thirty-three of 35 hepatoma patients had associated cirrhosis of the liver. Sixty per cent of hepatomas smaller than 5 cm in diameter were invisible at surgery. Three hyperplastic nodules in the cirrhotic liver and three cavernous hemangiomas were also invisible because of the lesions being embedded in the liver parenchym . The sonographic examination proved to be a useful method not only for knowing the exact localization of invisible tumors but also for preventing vascular injury at subsegmental or segmental resection of the liver.

Adenoma, Bile Duct↗
PubMed1982

Pericoronary nerve mediates inhibitory sympathetic response to coronary occlusion.

We determined the effect of pharmacological blood of the pericoronary nerve either along the experimentally occluded left circumflex (CX) or the nonoccluded left anterior descending coronary artery (LAD) on the reflex inhibition of renal nerve activity (RNA) during a brief CX occlusion. In 15 pentobarbital-anesthetized dogs with carotid sinuses denervated, CX was occluded under four different conditions of the pericoronary nerves. With intact pericoronary nerves, CX occlusion resulted in a significant decrease [-44 +/- 6.4 (SE) %] in RNA. The decrease in RNA remained basically the same (-34 +/- 10.0%) after block of the pericoronary nerve along LAD with local infiltration of 2% lidocaine. However, the reduction of RNA was abolished after block of the pericoronary nerve along CX (-6 +/- 4.9%) and after block of the pericoronary nerves along both LAD and CX (3 +/- 2.6%). These results indicate that the afferent input transmitted via the pericoronary nerve along the experimentally occluded coronary artery plays a dominant role in mediating the reflex inhibition of RNA during the coronary occlusion.

Animals↗