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S Slavin

Publications and source records attributed to S Slavin.

At least 361 records · Page 20Linked to original sources
PubMed1980

The role of the spleen in tumor growth kinetics of the murine B cell leukemia (BCL1).

BCL1 is a transplantable B cell leukemia resembling human chronic lymphocytic leukemia-lymphoma maintained by cell passage in BALB/c mice. After BCL1 inoculation (10(7) cells), all mice developed extreme B lymphocytosis in the blood (less than or equal to 440,000 lymphocytes/mm3) and marked splenomegaly (50 times normal nucleated cell numbers). BCL1 infiltrated the spleen before peripheral leukemia was overt (3 days vs 28 days, respectively). BCL1 development in splenectomized mice was characterized by a delayed onset of leukemia (greater than 20,000 cells/ mm3 at 59 vs 28 days in intact mice), doubled median survival (102 vs 53 days, respectively), and reduced peak level of leukemic counts in the blood (125,000 vs 440,000 cells/mm3). Early splenectomy at different time intervals, ranging between 1 hr to 3 days after BCL1 inoculation, significantly delayed onset of the disease and prolonged survival, indicating that homing to the spleen occurred as early as 1 hr after inoculation. Splenectomy at 7 days still delayed onset of leukemia but did not affect survival. No significant effect on BCL1 kinetics was noticed when splenectomy was done on day 21. All splenectomized mice showed significantly lower peripheral blood counts as compared to intact mice (98,000/mm3 vs 440,000/mm3, respectively). The data show that the spleen plays a major role in the pathogenesis and prognosis of BCL1.

Animals↗
PubMed1979

Is there postdefecation bacteremia?

An investigation was done to determine the incidence of postdefecation bacteremia. A study of 82 healthy volunteers was done. Among 164 predefecation cultures, only one (0.6%) from an anaerobic flask was positive for microorganism, which was identified as Staphylococcus epidermidis. In only two of 328 (0.6%) postdefecation cultures did microorganisms grow, which were shown to be Propionibacterium acnes. The results of this study suggest that either bacteremia after defecation does not occur or is a rare event in healthy individuals.

Adolescent↗
PubMed1979

Successful treatment of autoimmune disease in (NZB/NZW)F1 female mice by using fractionated total lymphoid irradiation.

Adult female (NZB/NZW)F1 hybrid mice with documented autoimmune glomerulonephritis resembling systemic lupus erythematosus were treated with fractionated total lymphoid irradiation (TLI), a modification of the radiotherapeutic regimen currently used for the treatment of Hodgkin disease. After TLI, proteinuria subsided in all mice that had undergone radiotherapy, and the mean survival increased from 359 days in untreated controls to 545 days. It is suggested that TLI should be further investigated as a new approach for immunoregulation of autoimmune disorders.

Animals↗
PubMed1979

Single and double stranded DNA binding lymphocytes in the peripheral blood of patients with systemic lupus erythematosus and normal controls.

Specific double (D-DNA) and single stranded (S-DNA) deoxyribonucleic acid binding cells were demonstrated in the peripheral blood lymphocytes of patients with systemic lupus erythematosus (SLE) by rosette formation with antigen coated red blood cells. The proportion of DNA binding cells in the peripheral blood of patients with SLE was significantly higher than that found in a random population of healthy individuals. Significant numbers of D-and S-DNA binding lymphocytes were found in patients with active disease even when anti-DNA of fluorescent antinuclear antibodies disappeared. The specificity of the DNA binding cells was confirmed by inhibition experiments with D-or S-DNA. Spleen lymphocytes were also examined on one occasion and were found to contain a much higher level of DNA binding lymphocytes than the peripheral blood lymphocytes.

Antibodies, Antinuclear↗
PubMed1979

Characterization of the spontaneous murine B cell leukemia (BCL1). III. Evidence for monoclonality by using an anti-idiotype antibody.

We have raised an anti-idiotypic antibody against the cell surface IgM of the murine BCL1 tumor cells. This antiserum reacts exclusively with the IgM expressed on the tumor cells and detects a unique population of cells in the spleen and blood of the tumor-bearing mice. When these cells are stimulated in vitro with LPS, they secrete an IgM bearing the same idiotype as the cell surface Ig. These results are discussed in terms of a model for the immunotherapy of a chronic lymphocytic leukemia-like syndrome in mice.

Animals↗
PubMed1979

Induction of allograft tolerance after total lymphoid irradiation (TLI): development of suppressor cells of the mixed leukocyte reaction (MLR).

We searched for the presence of suppressor cells of the MLR in C57BL/Ka leads to BALB/c chimeras. The chimeras were made with total lymphoid irradiation (TLI) and marrow transplantation. Spleen cells from the old chimeras inhibited the MLR of BALB/c responder cells against C57BL/Ka stimulator cells. Inhibition was specific for the stimulator cells, since no effect on the MLR was observed with C3H or BALB.C3H stimulator cells. Maximal inhibition was achieved when the responder cells in the MLR shared the H-2 haplotype of the chimeric recipient. Spleen cells obtained from chimeras young 30 to 40 days after BM transplantation inhibited the MLR nonspecifically, since similar marked inhibition was observed regardless of the H-2 haplotype of the responder or stimulator cells. The finding of antigen-specific and nonspecific suppressor cells is similar to that observed in mice rendered tolerant to bovine serum albumin after treatment with TLI.

Animals↗
PubMed1979

Characterization of a spontaneous murine B cell leukemia (BCL1). I. Cell surface expression of IgM, IgD, Ia, and FcR.

The surface marker expression of a spontaneous B lymphocyte leukemia discovered in a BALB/c mouse (BCL1) was examined and found to include a subset of markers known to occur on normal B lymphocytes. The tumor cells bore surface Ig that included both mu- and delta-chains associated with the lambda light chain. Alloantigens coded for within the murine MHC, including H-2D, H-2K, and I-region products, were identified on the tumor cells. Although normal B lymphocytes are thought to express products coded for within both the I-A and I-E subregions, the BCL1 expressed only normal amounts of I-E subregion products. In addition, the H-2 and Ia antigens revealed by 2-dimensional gel electrophoresis exhibited an abnormal pattern of post-translational modifications. The Fc, but not the complement-receptor, was present on the surface of tumor cells. The presence of IgD, Ia antigens, and the responsiveness to lipopolysaccharide (see subsequent paper) have led us to postulate that the BCL1 tumor represents a later differentiative stage than murine B lymphocyte tumors previously described.

Animals↗
PubMed1979

The pathology and homing of a transplantable murine B cell leukemia (BCL1).

The pathology and homing characteristics of a murine B cell leukemia are described. Experiments utilizing autoradiography to determine the early homing pattern of the leukemic cells revealed a pronounced localization of the labeled cells to the spleen. The cells that were seen in the white pulp showed preferential localization to the follicles or B cell domains. Tissue section immunofluorescence with antibodies to kappa- and lambda-light chains was used to study the initial mouse with this disease as well as to study the mice that were injected with in vivo passaged cells. These mice also showed predominant involvement of the spleen. Although the initial mouse with this disease had 200,000 lambda-bearing B lymphocytes per mm3 in the peripheral blood and closely resembled a human chronic lymphocytic leukemia patient, the studies described suggest that this murine B cell neoplasm is a lymphoma with a striking predilection for splenic involvement. The other organs including the bone marrow as well as the peripheral blood appeared to be involved secondarily. This unusual spontaneously occurring murine B cell disease provides a useful model for the investigation of certain commonly occurring human lymphomas and leukemias.

Animals↗