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Biomedical subjects

S Sinha

Publications and source records attributed to S Sinha.

At least 487 records · Page 27Linked to original sources
PubMed1982

Assessment of Dharmendra antigen. (V) attempts for purification of specific skin delayed hypersensitivity (DH) inducing antigen (S) from lepromin.

After electrophoresis (in pyrogen-free conditions) of Dharmendra antigen sonicates on agarose gel slides, the components (anionic and cationic) were eluted in pyrogen-free normal saline. On skin testing, it was found that only anionic component produced specific skin DH reactions in tuberculoid leprosy patients. The significance of the observations is discussed.

Antigens↗
PubMed1982

Assessment of Dharmendra antigen. (VI) skin tests with fractionated nucleic acids.

Leprosy patients were skin tested for induction of skin delayed type hypersensitivity (DTH) with various preparations of Dharmendra Antigen (DA) (sonicated antigen, DNA + RNA and DNA). Extracted nucleic acids produced smaller reactions (including some negative ones) as compared to sonicated whole antigen (which included proteins). BCG fractions, prepared similarly, produced non specific skin reactions.

Antigens↗
PubMed1981

Assessment of Dharmendra antigen. (IV) Antigenic analysis of lepromins.

In order to pinpoint the active portion of the skin antigens including Dharmendra and other lepromins, a study was carried out by subjecting the antigens to electrophoretic and immunoelectrophoretic analysis. These analyses identified two components, anionic and cationic, in lepromin sonicates. The anionic component was stainable with a protein strain, whereas cationic component formed precipitin arcs on immunoelectrophoresis with sera from lepromatous leprosy patients. BCG sonicate could also be resolved into two similar components. The cationic component of BCG apparently a glycoprotein, cross reacted with cationic component of lepromin and also showed a reaction of identity in gel diffusion. The specificity of anionic (proteinaceous) component of lepromin remains to be established.

Anions↗
PubMed1979

Tardive dyskinesia--reversible and persistent.

Twenty-one hospitalized patients over 50 years of age who had tardive dyskinesia were studied for 13 months. After withdrawal of neuroleptics and antidepressants for three months, dyskinetic symptoms abated in 12 patients and persisted in nine. Discriminant function analysis showed that the persistent and reversible dyskinesia groups could be clearly separated and that the best discriminator was the number of drug-free intervals. The persistent dyskinesia group had had significantly longer neuroleptic treatment (mean, 10.8 yr) and a greater number (mean, 5.6) of drug interruptions of at least two months' duration each than did the reversible dyskinesia group. Our finding, as well as the literature reviewed, does not support the commonly held notion that frequent lengthy interruptions of long-term drug treatment reduce the incidence of persistent dyskinesia, at least in patients who are otherwise predisposed to the development of tardive dyskinesia.

Aged↗
PubMed1979

Immunological assessment of sera of leprosy patients.

IgG levels were significantly high in sera of all types of leprosy. House-hold contacts of lepromatous leprosy (LL) cases also showed significantly higher values for IgG when compared to that of control. Except polar tuberculoid (TT) cases and house-hold contacts other types of leprosy revealed a significant rise in IgA levels in their sera. IgM was only raised in borderline tuberculoid (BT) cases. C-reactive protein (CRP) was present in the sera of all types of leprosy. Highest positivity (97%) was shown by sera from erythema nodosum leprosum (ENL) cases. Rose-Waaler antibody (RA) was noted in BT, borderline leprosy (BL), LL and ENL cases. Significance of these findings is discussed.

C-Reactive Protein↗
PubMed1979

Assessment of Dharmendra antigen. III. Comparative study with Mitsuda antigen.

Four fractions each from Dharmendra and Mitsuda antigen have been obtained by step-wise centrifugation and sonication of the antigen. These fractions have been assessed for their capacity of inducing skin delayed hypersensitivity response. While, it has been noted that all fractions of both types of antigens can induce a good early reaction, the late skin reaction is only mounted by intact bacilli of both types of antigen. When compared at a constant bacillary concentration, Dharmendra antigen has produced better early skin reaction than Mitsuda antigen, whereas the intensity of late skin reaction is almost equal with both the antigens. The hypothesis has been put forward that the early, as well as the late reaction are produced by the same antigen and this antigen is located in the protoplasm of M. leprae.

Antigens, Bacterial↗
PubMed1978

Production of amylase by a submerged culture of Aspergillus wentii.

Soluble starch was hydrolysed to maltose by Aspergillus wentii Wehmer (IMI 17295). Studies on nutritional requirements of Aspergillus wentii for production of amylase revealed that the optimum conditions were achieved in fermentation culture medium containing 1% starch, and incubated at 20 degrees C for 3 days at pH 6.0. Tryptophan was the best nitrogen source. The amylase activity was completely inhibited when 1 mM sodium iodoacetate was incorporated into the medium. With 10 mM sodium citrate the amylase activity was increased from 3.51 to 6.0 mg/ml.

Amylases↗
PubMed1978

Cholinergic agents inhibit sodium transport across the isolated toad bladder.

Acetylcholine and carbamylcholine (carbachol) inhibited sodium transport across the toad bladder. This effect was blocked by atropine. Carbachol increased the uptake of 45Ca by isolated toad bladder epithelial cells. This increased 45Ca uptake was blocked by atropine, pentobarbital, or lanthanum chloride. The inhibitory effect of cholinergic agents on sodium transport was dependent on external calcium concentration and was abolished by decreasing external calcium from 2 to 0.2 mM or by agents (pentobarbital, lanthanum chloride) which prevent the movement of calcium into cells. Carbachol increased modestly, but significantly, the levels of cyclic GMP in isolated toad bladder epithelial cells. This effect was blocked by atropine. Whether the decrease in sodium transport produced by cholinergic agents is the result of increased intracytoplasmic calcium levels, increased cyclic GMP levels, or a combination of both remains to be established.

Acetylcholine↗
PubMed1978

Ultraviolet induced mutations in Aspergillus wentii.

Aspergillus wentii (IMI 17295) and its three nutritional mutant strains were irradiated with UV rays. New mutants obtained differed from the parent strains in colour of the conidia, growth factor requirements and amylase activity. Arginine deficient strains showed greater amylase activity.

Amylases↗