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Biomedical subjects

S Shindo

Publications and source records attributed to S Shindo.

At least 109 records · Page 6Linked to original sources
PubMed1987

Improved patency of collagen-impregnated grafts after in vitro autogenous endothelial cell seeding.

Currently available prosthetic vascular grafts remain sufficiently thrombogenic to preclude their use as small-caliber arterial substitutes. However, thrombogenicity may be significantly reduced by the presence of an endothelial monolayer on the luminal surface. The present study was undertaken to test the efficacy of lining a small-caliber prosthesis with autogenous endothelial cells in vitro so that the graft may subsequently be implanted with an established confluent endothelial lining. For this purpose, cells were obtained from canine external jugular vein, harvested enzymatically, and passaged in culture. Dacron grafts (4 X 150 mm) were then seeded in vitro and maintained for 48 to 72 hours before implantation in the femoral position of the same animal. Seeded grafts were implanted contralateral to unseeded control grafts and explanted after 1 month. Seeded grafts demonstrated an 86% patency rate at explanation in contrast to the significantly lower 14% patency rate of the unseeded control grafts. This study justifies further investigation directed toward the feasibility of endothelializing intravascular prostheses in vitro before implantation.

Animals↗
PubMed1986

Effects of starvation on guanidino compound metabolism in mice.

Arginine levels diminished markedly in the plasma during starvation, suggesting that plasma arginine level principally depends on food intake. Organ arginine levels were relatively stable except for an extraordinary increase in the pancreas at 96 h. Guanidinoacetic acid decreased dramatically in all organs within 24 h and low level were maintained thereafter, except for the brain (and plasma). Creatinine output increased after 24h of starvation. The increased creatinine output recovered to the control level after 48 h. A small but significant amount of guanidinosuccinic acid was detected in the normal liver and decreased transitorily after 24 h and increased after 48 h starvation, corresponded with an increased output in the 24 h urine excretion. Otherwise this decrease may be related to the transitory decrease in arginine level in the liver over the same time course. Methylguanidine in the muscle and gamma-guanidinobutyric acid in the liver decreased gradually during starvation. These results suggest that guanidino compounds levels in mouse organs are principally dependent on exogenous nitrogen.

Animals↗
PubMed1985

Identification of 2-guanidinoethanol in human urine.

Guanidino compounds in normal human urine were analyzed by high-performance liquid chromatography; an unknown peak was observed in the chromatogram that was identical to the peak of synthetic 2-guanidinoethanol. In another experiment, the substance was purified from human urine by successive use of strongly acidic ion-exchanger, thin-layer chromatography and then weakly acidic ion-exchanger. After this it was reacted with acetylacetone to form dimethylpyrimidyl derivative. After further reaction of this derivative with trifluoroacetic anhydrate, it was analyzed by gas chromatography/mass spectrometry. The mass chromatogram and mass spectrum were identical to those of the trifluoroacetylated dimethylpyrimidyl derivative of synthetic 2-guanidinoethanol. This is the first report on the identification of 2-guanidinoethanol in human urine. The concentration of 2-guanidinoethanol in the urine of healthy humans was 5.7 +/- 1.8 (mean +/- SD) mumol/g creatinine.

Adult↗
PubMed1985

[Current problems in the surgical treatment of acquired heart disease].

Two hundred and ninety patients with ischemic heart disease and two hundred and sixty one patients with valvular heart disease have been operated upon in our department. Combined valvular and coronary artery surgery was carried out in 19 cases, coronary artery and peripheral vascular surgery was carried out in 7 cases, and tricuspid valve replacement with SJM was carried out in 20 cases. Early mortality rate of combined valvular and coronary artery surgery was 26%, and late mortality 21%, however, only 3 cases (15.8%) died of LOS. Mortality rate of combined coronary artery and peripheral vascular surgery was 43%, however 1 patient died of LOS. Mortality rate of TVR with SJM was 15% which was rather low in comparison with other valves. A patient who underwent simultaneous surgical treatment of valvular or peripheral vascular disease, and coronary artery disease, ran almost the same risk of LOS as patients without ischemic heart disease. However, myocardial protection was important for these patients because of severe LV dysfunction and myocardial hypertrophy. There is not yet an ideal artificial valve for TVR. At present, SJM is the best available valve in terms of design and hemodynamics.

Adult↗
PubMed1983

Evolution of renal segmental atrophy (Ask-Upmark kidney) in children with vesicoureteric reflux: radiographic and morphologic studies.

A radiographic and morphologic study of nine patients with renal segmental "hypoplasia," whose kidneys were radiographically normal when first examined, showed the lesion to be a form of localized, progressive renal atrophy. The renal abnormality bears a strong relationship to vesicoureteric reflux, which could be demonstrated in every patient. No evidence of renal scarring or atrophy was observed in initial roentgenographic examinations at a mean age of 2.9 years (range 0.1 to 10 years); however, serial studies during five to 14 years after discovery of vesicoureteral reflux demonstrated both a lack of renal growth and a progressive loss of substance irrespective of infection. Radiographic lengths of scarred kidneys, as measures of renal growth, correlated poorly with the radiographic surface areas of the renal parenchymal outlines. The mean time from discovery of vesicoureteric reflux to appearance of a renal scar was 6.1 years, and to onset of hypertension in six patients was 7.8 years. The renal abnormality consisted of lobar atrophy with variable tubular atrophy and glomerular sclerosis and with parenchymal destruction that in some specimens had proceeded to a complete loss of nephronic elements. The occasional presence of relatively well-preserved glomeruli and tubules and of focal segmental sclerosis within persisting glomeruli was taken as evidence of a progressive renal abnormality, as opposed to a static developmental hypoplasia. These observations indicate that renal scarring, the injury presumably having been initiated by vesicoureteric reflux, can progress despite correction of the reflux and despite prevention of urinary tract infection.

Adolescent↗