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Biomedical subjects

S Shimizu

Publications and source records attributed to S Shimizu.

At least 811 records · Page 45Linked to original sources
PubMed1988

Molecular effects of antiestrogens (tamoxifen and LY117018) on estrogen-dependent glycoprotein (USP-1) synthesized and secreted by rat uterine epithelial cells.

We have previously identified and characterized a 97K secretory glycoprotein (uterine secretory protein-1:USP-1) synthesized and secreted by rat uterine epithelial cells under estrogen stimulation. We now have analyzed the qualitative and quantitative effects of antiestrogens (tamoxifen and LY117018) on the induction of USP-1 biosynthesis when administered alone or combined with 17 beta-estradiol (E2). By radioimmune precipitation assay of [35S]methionine-labeled uterine luminal fluid proteins, it was shown that tamoxifen and LY117018 could weakly induce USP-1 compared with E2. Concomitant administration of either tamoxifen or LY117018 with E2 significantly diminished the effects of E2 on USP-1 induction. Hence, both tamoxifen and LY117018 possess agonistic as well as antagonistic properties affecting the induction of USP-1. These agonistic effects of antiestrogens were also evident from the presence of USP-1 in rat uterine epithelial cells treated with antiestrogens, as revealed by immunohistochemical staining. Sodium dodecylsulfate-polyacrylamide gel analysis of immunoprecipitable [35S]methionine-labeled protein revealed that USP-1 induced by tamoxifen is larger (110K) than that induced by estrogen or LY117018 (97K). Peptide-N-glycosidase treatment of USP-1 induced by E2 or tamoxifen removed asparagine-linked carbohydrate chains and resulted in the appearance of polypeptides with apparent mol wt of 91K and 105K, respectively. Thus, the higher mol wt of tamoxifen-induced USP-1 is not due to changes in asparagine-linked carbohydrates. Since LY117018 could not induce any qualitative change in the USP-1 molecule, tamoxifen may act on uterine epithelial cells through a different molecular mechanism than LY117018.

Animals↗
PubMed1988

Hyaluronic acid-dependent change in the extracellular matrix of mouse dermal fibroblasts that is conducive to cell proliferation.

Fluorescein isothiocyanate (FITC)-labelled hyaluronic acid, when incubated with subconfluent cultures of mouse dermal fibroblasts, was incorporated into the extracellular matrix. Deposition of the fluorescent label reached the maximum about 48 h after its addition. Hyaluronic acid decasaccharide, but not octasaccharide, inhibited the incorporation of the fluorescent label, suggesting that at least 10 sugar units in length are necessary for the incorporation of hyaluronic acid. A 2 M-urea extract of the cell layer had the ability to bind [3H]hyaluronic acid. Again, the binding was inhibited by hyaluronic acid decasaccharide but not by octasaccharide, suggesting the presence in the urea extract of a hyaluronic acid-binding molecule that may participate in the incorporation of hyaluronic acid. A supramolecular aggregate prepared by rate-zonal sedimentation from the 2 M-urea extract contained chondroitin sulphate proteoglycans capable of interacting with hyaluronic acid. Their core molecules were identical in size with those from a hyaluronic acid-binding chondroitin sulphate proteoglycan (PG-M) previously described in chick embryo fibroblasts. Immunofluorescence analyses with anti-proteoheparan sulphate antibodies indicated that both exogenous addition of hyaluronic acid and enhanced synthesis of hyaluronic acid caused a preferential decline in the proteoheparan sulphate level in the extracellular matrix. Subsequent to this change, the cells began transient DNA synthesis. We suggest that hyaluronic acid-dependent modulation of the level of proteoheparan sulphate in the extracellular matrix could be a causal event of cell proliferation.

Animals↗
PubMed1988

Inhibition of synaptosomal (Na+ + K+)-ATPase activity by lysophosphatidic acid: its possible role in membrane depolarization.

Lysophosphatidic acid (LPA), a metabolite of phosphatidic acid (PA) by phospholipases of the A2 type, markedly inhibited the synaptic membrane (Na+ + K+)-ATPase activity from rat cerebral cortices in incubation media containing free Ca2+ concentrations below 4.7 x 10(-6) M. This effect of LPA was dose-dependent, and the minimum effective concentration was 10(-6) g/ml. The inhibitory action of LPA was more potent than that of PA. Lysophosphatidylinositol (LPI) and lysophosphatidylethanolamine (LPE) at 10(-5) g/ml had little or no effect on this enzyme activity. These results suggest that LPA may partly play a role in the depolarization and/or increase in intracellular calcium concentrations in the brain.

Animals↗
PubMed1988

Monoclonal gammopathies in patients with Sjögren's syndrome.

We studied 18 sera of Sjögren's syndrome (SS) with monoclonal gammopathy. Monoclonality was established by typical immunoelectrophoretic findings in all patients and confirmed by idiotypic (Id) studies in 12 patients. Four of the monoclonal gammopathies were of the IgG class, 8 were of the IgA class and 4 were of the IgM class, and 2 patients had 2 M proteins (IgMK/IgGK and IgAK/IgGK). The monoclonal rheumatoid factor (RF) was found in 6 patients (4 IgA and 2 IgM). A review of the literature revealed additional 19 monoclonal gammopathies (2 IgG, 9 IgA, 7 IgM and one Bence Jones protein) in Japanese SS patients. In non-Japanese SS patients, 27 monoclonal gammopathies (4 IgG, 2 IgA, 20 IgM and once Bence Jones protein) were reported. Both Japanese and non-Japanese patients showed a higher incidence of monoclonal gammopathies in primary than in secondary SS. The non-IgM class monoclonal gammopathies were predominant in Japanese SS patients, whereas monoclonal gammopathies were mostly confined to the IgM class in non-Japanese SS patients. These results indicate that monoclonal gammopathy is another significant complication of SS.

Aged↗
PubMed1988

Interleukin-6 (B-cell stimulatory factor 2)-dependent growth of a Lennert's lymphoma-derived T-cell line (KT-3).

A T lymphoma cell line (KT-3) established from a patient with Lennert's lymphoma showed macrophage-dependent growth. Macrophage-derived factors were able to replace the macrophage functions. Experiments using a variety of cytokines demonstrated that KT-3 proliferated in response to recombinant interleukin-2 (rIL-2), rIL-4, or rIL-6 but did not proliferate in response to rIL-1 alpha, rIL-1 beta, rIL-3, recombinant granulocyte colony-stimulating factor (rG-CSF), rGM-CSF, recombinant interferon-alpha (rIFN-alpha), rIFN-gamma, recombinant tumor necrosis factor (rTNF-alpha), or native IFN-beta. Polyclonal rabbit anti-IL-6 antibody almost completely neutralized the activities of macrophage-derived factors or IL-6 but not IL-2 or IL-4. Scatchard plot analysis demonstrated that KT-3 cells indeed express IL-6 receptors. The results indicate that the macrophage-derived factor that supports the growth of KT-3 is IL-6 and suggest that macrophage-derived IL-6 may play an important role in the histopathogenesis of Lennert's lymphoma.

Antigen-Antibody Reactions↗
PubMed1988

An established CD4+ T lymphoma cell line derived from a patient with so-called Lennert's lymphoma: possible roles of cytokines in histopathogenesis.

A T lymphoma cell line, KT-3, was established from the peripheral blood of a patient with so-called Lennert's lymphoma when the patient developed leukemic lymphoma. The KT-3 cells stain positively for alpha-naphthyl acetate esterase, PAS, and acid phosphatase. The surface phenotype is E rosette-positive, CD1-, CD2+, CD3-, CD4+, CD5+, CD8-, CD11-, CD20-, CD25 (anti-Tac)+, OKla-1+, HNK-1-, J-5-, and My9-, and the surface membrane immunoglobulin is negative, which is almost the same phenotype as the leukemic cells studied when the culture was started. Southern blot analysis showed rearrangement in both beta and gamma T cell receptor genes. Although KT-3 cells proliferate spontaneously and form clusters, they cease proliferating when they are cultured at low cell densities. They proliferate vigorously in response to macrophages, macrophage-derived factor, or recombinant interleukin 2 (rIL-2) added to the culture. Furthermore, they secrete interferon-gamma (IFN-gamma) spontaneously, and the secretion is augmented when they are stimulated with macrophage-derived factor. The macrophage-derived factor enhancing KT-3 cell growth is different from IL-1 alpha, IL-1 beta, IL-2, or IFN-gamma. To our knowledge, this is the first tumor cell line established from a patient with Lennert's lymphoma. The results conclusively confirmed that, at the least, Lennert's lymphoma included CD4+ T lymphoma and also suggested that cytokines or factors secreted by T lymphoma cells and epithelioid histiocytes play an important role in the histopathogenesis of Lennert's lymphoma.

Antigens, Neoplasm↗
PubMed1988

Isolation of Theileria sergenti piroplasms from infected erythrocytes and development of an enzyme-linked immunosorbent assay for serodiagnosis of T sergenti infections.

A method of obtaining a pure suspension of Theileria sergenti piroplasms from infected bovine erythrocytes was developed and the resulting parasites used as antigen in an enzyme-linked immunosorbent assay (ELISA). Piroplasms were freed from infected erythrocytes using the nitrogen cavitation technique and then separated from unbroken erythrocytes by centrifugation at 670 gmax. The parasites in the supernatant were then sedimented by centrifugation at 2700 gmax and the purified fraction examined by electron microscopy. This examination showed that the isolated piroplasms were morphologically intact and that there were few contaminants. SDS-PAGE and spectrophotometric analysis showed that this fraction contained little erythrocyte component. The piroplasms thus obtained were sonicated and treated with Triton X-100 then used for ELISA antigen. The ELISA values had a high correlation with titres obtained using the indirect fluorescent antibody test on sera from cattle infected with T sergenti.

Animals↗
PubMed1988

[A case of gastric metastases from carcinoma of the breast].

Reported is a case of a gastric metastases from a cancer of the breast in a 54-year-old woman who had received a standard radical mastectomy for a cancer of the right breast (T2N1bM0). Microscopic examination had revealed a lobular carcinoma, partially showing a signet ring appearance, and metastases in 4 axillary lymph nodes. Two years and 6 months later as a result of an upper GI series and a gastroscopy, linitis plastica was observed. Because there were no other metastatic lesions, a laparotomy was performed. The stomach, however, was unresectable. Further, a nodal biopsy revealed a metastatic breast cancer. Because of the probe laparotomy, however, the patient's symptoms were aggravated for several months. Death occurred three years and five months after the mastectomy. An autopsy revealed systemic metastases which included the stomach that had originated from her breast.

Breast Neoplasms↗
PubMed1988

Cellular mechanisms of hypertension and atherosclerosis: hypoxia-induced lipid accumulation in cultured vascular smooth muscle cells from the stroke-prone spontaneously hypertensive rat.

The effects of hypoxia on the lipid metabolism of cultured vascular smooth muscle cells from stroke-prone spontaneously hypertensive rats (SHRSP) were examined. The total cholesterol in smooth muscle cells cultured with hyperlipidaemic serum reached higher levels under hypoxic conditions (3.5% O2, 5.0% CO2, 91.5% N2) than under normal conditions (20% O2, 5.0% CO2, 75% N2). In normal serum, cholesterol increased slightly under hypoxic conditions. No difference was noted in cholesterol synthesis from [14C]-acetic acid. Triglyceride levels in smooth muscle cells cultured with hyperlipidaemic serum and normal serum were significantly increased compared with fetal calf serum. The increase in triglyceride levels was inhibited markedly under hypoxic conditions. Triglyceride synthesis induced by normal serum in the hypoxic cells was decreased compared with control cells. The metabolic rate of triglyceride did not differ between the two. These results indicate that hypoxic conditions accelerate the cellular uptake of cholesterol, an initial step in atherogenesis.

Animals↗
PubMed1988

[Experiences with sultamicillin granules in pediatric patients].

Sultamicillin (SBTPC) is a combination drug of sulbactam (SBT) and ampicillin (ABPC) in an ester bonding at 1:1 ratio. SBT, a new semisynthetic beta-lactamase inhibitor restores and extends the spectrum of ABPC against resistant strains of bacteria. SBTPC granules were administered to 26 pediatric patients with infections, and clinical efficacies were studied in 23 patients. The clinical efficacy rate was 95.7% and the drug was evaluated to be highly effective for the treatment of infectious diseases in the pediatric field. SBTPC was safe and well tolerated.

Age Factors↗