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Biomedical subjects

S Shibasaki

Publications and source records attributed to S Shibasaki.

46 records · Page 3Linked to original sources
PubMed1978

A fine structure study of the non-ciliated cells in the mouse tracheal epithelium with special reference to the relation of "brush cells" and "endocrine cells".

The non-ciliated epithelial cells in the air way of mice were examined by electron microscopy. They are flask-like or pyramidal cells dispersed singly or occasionally in pairs. The apical portion of the cells reaches the air way lumen with microvillous projections. Their cytoplasm always contains abundant and variable numbers of small granules (60-170 nm in diameter). Close appositions between non-ciliated cells and nerve fibers are frequently observed at their lateral or basal surfaces. Fine structures of the non-ciliated cells are similar to those previously reported in the cells under different designations such as brush cells, basal-granulated cells, cells of neuroepithelial bodies and endocrine cells. The present study proposes that these names express different features of the same cell group collectively called "non-ciliated cells" in this paper. Furthermore images indicating exocytotic granule release are obtained in this study, indicating an endocrine function of these cells.

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PubMed1976

Light and electron microscopic study on the endocrine cells of the pancreas in a marine teleost, Fugu rubripes rubripes.

The pancreatic endocrine tissue of Fugu rubripes rubripes consists of numerous round principal islets (Brockmann bodies) of various sizes scattered around the gall-bladder. The endocrine cells are divided into A-, B-, D-, and Ff-cells. Each cell type was identified by comparing thick and thin sections in both light and electron microscopy. Aldehyde-fuchsin positive B-cells contain numerous round secretory granules (average diameter 300 nm) each of which has a round compact core of moderate density; a narrow space exists between this core and the limiting membrane. Grimelius' silver positive A cells contain round secretory granules (average diameter 360 nm) with a hexagonal or tetragonal crystalline core (average diameter 170 nm) of high density; the silver grains perferentially appear in the space between the limiting membrane and the core. The crystalline core of each alpha-granule often contains an appendix like structure of variable shape. D cells blackened by the silver impregnation method of Hellman and Hellerström (1960) have round secretory granules (average diameter 320 nm) filled with a flocculent material of low density. The fourth cell type (Ff-cell) has a clear cytoplasm after differential staining for light microscopy. By electron microscopy, this cell has elongated fusiform secretory granules (520 nm average length x 230 nm average width) filled with numerous filaments arranged in parallel with the longitudinal axis. Figures suggesting granule formation in the sacs of the Golgi apparatus were obtained in all of islet cell types. Equivalents of emiocytotic release of secretory granules were encountered in the A and Ff cells.

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