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Biomedical subjects

S Sharma

Publications and source records attributed to S Sharma.

At least 1,243 records · Page 69Linked to original sources
PubMed1987

Antipili antibody affords protection against ascending pyelonephritis in rat: evaluated by renal brush border membrane enzymes.

Kinetic parameters (Km and Vmax) of renal brush border membrane (BBM) enzymes alkaline phosphatase, maltase, leucine aminopeptidase and gamma-glutamyltranspeptidase were worked out in control, infected and immunized-infected rats. There was no significant change in the Km of all the enzymes studied in three groups. The Vmax of all the enzymes studied decreased significantly (p less than 0.05) 3 or 4 days postinfection and onwards in the left obstructed kidney of infected and immunised-infected animals. However, in the right unobstructed kidney the Vmax of alkaline phosphatase and leucine aminopeptidase increased significantly (p less than 0.05) in the early stages and decreased (p less than 0.05) in later stages in both the experimental groups. The significant difference (p less than 0.05) in the Vmax of infected and immunized-infected groups at various stages of infection revealed the partial protective role of antipili antibody against ascending pyelonephritis.

Alkaline Phosphatase↗
PubMed1987

Prevention of alterations in the transport of nutrients in pyelonephritic rats by immunization with pili.

The uptake of D-glucose, L-aspartate, L-lysine and L-proline was studied in renal brush border membrane vesicles prepared from control, infected and actively immunized-infected rats. The uptake of D-glucose, L-lysine and L-proline was decreased significantly (p less than 0.05) during the course of infection in the infected animals. However, the uptake of L-aspartate was increased significantly (p less than 0.05) in early stages and decreased significantly (p less than 0.05) in later stages of infection in the infected animals. When the animals were actively immunized with pili, still there were changes in the uptake of D-glucose and L-aspartate, but the changes appeared later and less pronounced. No change in the uptake of L-lysine and L-proline was observed in the immunized-infected animals. The findings demonstrated that active immunization with pili prevents alterations in the uptake of nutrients in pyelonephritic rats.

Animals↗
PubMed1987

Effect of contraceptives on the adhesion of Escherichia coli to uroepithelial cells.

We studied the effect of female ovarian hormones present in contraceptive preparations on six strains of Escherichia coli isolated from patients with urinary tract infections. These strains had variable attachment potential. To study this effect in vivo, we gave a woman an oral contraceptive preparation and then compared the amount of attachment to her uroepithelial cells before and after hormone administration. To study the in vitro effect, we exposed uroepithelial cells from this same woman to different hormone concentrations for up to 18 hr and noticed any alteration in adhesion. We observed that in vivo as well as in vitro exposure of uroepithelial cells to hormones enhanced the adhesion of all E. coli strains.

Adult↗
PubMed1987

Studies on the vasoocclusive crisis of sickle cell disease. III. In vitro and in vivo effect of the pyrimido-pyrimidine derivative, RA-233: studies on its mechanism of action.

Red cell deformability was found to be impaired in SS- and SC-genotype and to a lesser extent SA-genotype red blood cells as compared with those of AA-genotype. RA-233 in vitro improved deformability according to a bell-shaped dose-response curve. RA-233 also prevented experimental vasoocclusive crisis in Macaca arctoides. Deoxygenation of SS-genotype red cells resulted in sickle shape transformation, ATP depletion, potassium efflux, and attachment of hemoglobin molecules to the membrane. These changes were prevented by RA-233. Suspending SS-genotype red blood cells in potassium rich tris-buffer also prevented potassium efflux during deoxygenation and also decreased cellular deformability. RA-233 had no effect on osmotic fragility of SS-genotype red blood cells.

Adult↗
PubMed1987

In vitro studies on leukemia cells and T lymphocytes in hairy cell leukemia.

Hairy cell leukemia cell lines were established from eight untreated patients using purified B cell growth factor (BCGF) in vitro. These cell lines maintained their original cell surface immunophenotype for about 1 month, after which they began to lose one or more of their characteristic surface antigens. The cell lines also maintained typical hairy cell leukemia morphology for 2-3 months in vitro but later showed an increasing number of multinucleate giant cells that maintained a B cell surface phenotype. The cell lines became independent of exogenously provided BCGF after at least 1 month in vitro and secreted BCGF activity into culture supernatants in most cases. Some cell lines also acquired Epstein-Barr virus nuclear antigen positivity after variable period. Two hairy cell leukemia patients also showed hyperactive T cell responses in vitro and exhibited spontaneous T cell proliferation in culture without exogenously supplied interleukin-2. These T cell lines had the T helper phenotype and secreted significant amounts of T cell-associated lymphokines with BCGF and interleukin-2 activity into culture supernatants.

Antibodies, Monoclonal↗
PubMed1987

Synthesis and metabolic disposition of 14C-centperazine in rats.

The metabolic disposition of [2-14C]-centperazine (1; 3-ethyl-8-methyl-1,3,8-triazabicyclo[4.4.0]decan-2-one) following an injection of 5 mg/kg dose was studied in male albino rats. During the initial period of 45-60 min, a very rapid fall in blood level of radioactivity was noticed. Thereafter the rate of fall of blood radiocarbon went on decreasing but did not attain linearity even upto 24 h. The drug was found to be fairly distributed in all the tissues attaining highest concentration in most of them within 30 min. In 24 h, about 83.5% of the administered drug was eliminated from the body, of which 21.3% was recovered from the faeces and 61.1% from the urine. The 14CO2 accountes for only 1% of the total dose. The drug exhibited significant binding with all the tissues examined. The brain and muscle showed highest and lowest activity, respectively.

Animals↗