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Biomedical subjects

S Serrano

Publications and source records attributed to S Serrano.

At least 145 records · Page 8Linked to original sources
PubMed1981

[Response of growth hormone (GH) to glucagon-propranolol stimulation in diabetic subjects (author's transl)].

The functional study of growth hormone (GH) requires at least three different stimulation tests. To obtain a degree of hypoglycemia good enough to stimulate GH release is difficult in diabetic patients, and this fact has prompted us to utilize the stimulation induced by glucagon-propranolol. The test was performed in 15 diabetic patients and the results compared to those of 15 non-diabetic subjects. In the diabetic patients GH increased after stimulation from a mean basal level of 2.14 ng/ml to 15.32 ng/ml (p less than 0.01). Peak GH levels in plasma were reached between 120 and 180 minutes. Two failures were found and there were only minimal side effects. The results indicate that the test is valuable for the study of GH release in diabetic patients, and that it may substitute for the induction of hypoglycemia, which is more difficult to obtain in these patients. Finally, the results are compared to those obtained by other authors.

Adolescent↗
PubMed1976

[Our experience in the surgical treatment of pleural mesothelioma].

Surgery is of decisive importance in two situations: diagnosis insofar as thoracotomy offers, with very little risk, the opportunity to perform sufficiently ample biopsy; therapeutic because, lacking any other indicated approach, palliative intervention is recommended to stop effusion. 18 decortications (i.e. 40%) and 2 pleuropneumonectomies are reported. Results were good.

Biopsy, Needle↗
PubMed1972

Arterial spasms.

Explore the source record for details and available documents.

Animals↗
PubMedDate not supplied

[Usefulness of RET proto-oncogene in the diagnosis of hereditary-type medullary carcinoma of the thyroid. Correlation with surgical findings].

In about 25% of cases medullary thyroid carcinoma (MTC) is hereditary. In this group is possible to detect germline point mutations of the RET proto-oncogene in about 95% of the studied cases. The purpose of the present paper is to confirm the value of the RET in the screening of the hereditary MTC. We studied 43 subjects at risk for a Multiple Endocrine Neoplasia Type 2A Syndrome. RET analysis was positive for MEN 2A in 22 subjects. Fifteen of the 22 have undergone a total thyroidectomy at our facility. Histopathological study of the surgical specimens confirmed the presence of a MTC in all the cases. According with our experience. RET analysis is a 100% sensitive and specific method of hereditary MTC screening, and we think it has obvious advantages over the calcitonin tests in technical, economic and ethic aspects.

Adolescent↗
PubMedDate not supplied

[Bibliometric indicators of production and usage of Archivos de Bronchoneumologia].

We studied the bibliometric indexes referring to production and scientific output published in ARCHIVOS de BRONCONEUMOLOGIA. All issues published between 1993 and 1994 were studied with the exception of monógraphic or supplementary numbers. For each article we recorded article type, number of authors, authors' place of work, specialty, time until acceptance, time until publication, type of references, language of references, journal and year of references. For each year we calculated the productivity index and the cooperation index, or index of signers per work. We also determined the citation period for references, the Price index, and the insularity index. Productivity index was 1.97 in 1993 and 2.05 in 1994. The cooperation index for the two years reached 4.5 +/- 1.9 (1-10). Authors were widely distributed by provinces and by specialties, although "nuclei" of production could be identified. The citation half-life period was 5.67 years for references in 1993 and 5.94 years in 1994, and the Price index surpassed 40%. The insularity index was very low at 7.76%. In conclusion, the bibliometric indicators for production, readership and obsolescence for the two years analyzed indicate that ARCHIVOS de BRONCONEUMOLOGIA occupies an intermediate position in the ranking of Spanish medical journals.

Bibliometrics↗
PubMedDate not supplied

Electron microscopy in pathology articles: a retrospective appraisal.

Electron microscopy (EM) is a valuable standard tool in basic research and teaching. However, its use in diagnosis is limited, either for strategic reasons or budgetary constraints. This means that its many potential applications are more often neglected, either as an ancillary tool, quality control method, or gold standard, to complement, support, or confirm results of pathological studies. To evaluate the use of EM in this setting, the authors analyzed all articles (n = 2,531) in the three top indexed diagnostic pathology journals for a period of 60 months from July 1993 to June 1998. A total of 448 articles in which the use of EM was indicated, according to standard surgical pathology textbooks, were selected. Both the actual and the potential EM content of each article were scored, as follows: zero, illustrative, supportive, gold standard (for confirmation of research results), extensive, and predominant. Of the total number of articles in which EM was indicated, 77% made use of the technique. EM support was lacking most frequently in articles on serosal neoplasms and on new diagnostic strategies (p < .00005). There was no definite trend toward an increase or decrease in the use of EM during the period analyzed. The authors conclude that EM is used in most reports on diagnostic pathology, when it is indicated. However, a small but non-negligible percentage of articles (23%) could benefit from including EM as an ancillary, control, or gold standard method to complement, support, or confirm their results.

Diagnosis, Differential↗
PubMedDate not supplied

Intercellular junctions, apical differentiation, and infiltrative features in colon cancer: an ultrastructural study. The Colon Cancer Team at IMAS.

Changes in the structure and number of cell junctions have been related to the infiltrative and metastatic potential of tumor cells. Apparently, the loss of cell adhesion should be coordinated with significant changes in the apical and basal cell domains. The authors have performed a sequential ultrastructural study of cells in the superficial, middle, and deep regions of well- and moderately differentiated colon adenocarcinomas. This was to investigate the differences in the organization of different membrane domains among tumor cells in the in situ areas, the advancing, infiltrative edge of the tumors, and the infiltrating zones between these two extreme zones. The results of the study suggest that the organization of these domains is not strictly coordinated, and that, for each infiltration level, both a settling and an infiltrating cell population can be found. These findings could explain the fact that apparently well-differentiated tumors are able to seed distant tissues with individual cells, rather than with well-differentiated glandular aggregates that would hardly be able to reach the vessel lumina without significantly modifying their organization.

Actin Cytoskeleton↗
PubMedDate not supplied

Signet ring epithelioid stromal tumor of the small intestine.

Cells having a signet ring appearance can occur in mesenchymal, lymphoid, and other nonepithelial neoplasms. We report the case of an intestinal stromal tumor with smooth muscle differentiation and a prominent signet ring cell component. The presence of signet ring forms of smooth muscle cells in sections of paraffin-embedded tissue often contrasts with a lack of cytoplasmic spaces by electron microscopy, and the ultrastructural finding of signet ring-like areas in the present case can be attributed to the fact that the tissue for electron microscopy was retrieved from paraffin blocks where this peculiar artifact already existed. Ultrastructural examination of the signet ring-like areas suggests that they originated as retraction spaces which may have resulted from variations in intracellular tension forces related to the distribution of actin filaments.

Actins↗
PubMedDate not supplied

Clear cell meningioma of the lumbo-sacral spine with chordoid features.

Clear cell meningioma (CCM) is a peculiar variant that differs from conventional meningioma in affecting younger patients, arising more often in spinal or cerebellopontine locations, and showing a higher recurrence rate. Classical meningothelial areas are scarce in these tumors and the differential diagnosis with other neoplasms, particularly metastatic carcinoma, is often difficult. We report a case of clear cell meningioma from the lumbosacral spine in which location, radiologic presentation, light microscopic appearance in initial sampling, and some of the ultrastructural findings were reminiscent of chordoma. The tumor cells were diffusely positive for vimentin and very focally positive for epithelial membrane antigen. Ultrastructural demonstration of interdigitating cell processes joined by numerous desmosomes confirmed the diagnosis of CCM.

Adult↗
PubMedDate not supplied

Description of Opisthonecta matiensis n. sp. (Protozoa, Ciliophora), a new peritrich ciliate from wastewater.

Opisthonecta matiensis n. sp. was isolated from the inlet water of a wastewater treatment plant near Madrid, Spain, and studied in vivo, with silver methods, and using electronic and indirect immunofluorescence microscopy. This new species shows an amphora-like cell shape and has a size of 45-73 microns (x 58.2) x 25-40 microns (x 31.3). The oral infraciliature is formed by one haplokinety, three polykineties, and a short row of kinetosomes (epistomial membrane). The aboral infraciliature is made up of the trochal band and the scopula. From the trochal band arise three fibrillar systems: oral fibers, aboral fibers, and oblique fibers. The myoneme system is composed of a delicate peristomial ring, longitudinal branched fibers that reach the trochal band and of radial fibers extending from the scopula to the trochal band. The silverline system consists of an average of 147 lines. This new species is separated from other known forms by its smaller size, the presence of one single vacuole, and its higher number of silverlines.

Animals↗
PubMedDate not supplied

Differential expression of HLA class I and II antigens in primary and metastatic melanomas.

Class I and II histocompatibility antigen expression was studied in cryostat sections of biopsy tissues from 15 patients diagnosed as suffering from malignant melanoma, using monoclonal antibodies against HLA class I and II monomorphic determinants and an indirect immunofluorescence technique. Class I antigens were detected in three of the four primary melanomas and in five of the eleven metastatic melanomas. Class II antigens were expressed only in metastatic melanomas, in three out of eleven cases. Some tumour cell suspensions were obtained and short-term cultures were established. Radiobinding and immunoprecipitation studies were carried out in two cases, named M6 and M8. The results were comparable to those obtained with direct immunofluorescence. We modulated the expression of class I and II HLA antigens with interferon in M6 when adapted to tissue culture. This melanoma was class I and II negative; after IFN gamma treatment it became strongly positive for class I and II antigens. In addition we have demonstrated, using Southern blot analysis with the restriction enzymes PvuII and EcoRI, that the M6 melanoma does not have any detectable alterations in its class II beta genes.

Adult↗