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Biomedical subjects

S Seo

Publications and source records attributed to S Seo.

63 records · Page 4Linked to original sources
PubMed1980

Decrease in peritoneal mast cell count in rats with adjuvant arthritis. I. Time course and interstrain difference.

Adjuvant was injected into the left hind paw of Sprague-Dawley (SD) and Wistar rats. In SD rats a marked decrease in the mast-cell count and histamine content of the peritoneal fluid and a significant increase in the histamine content as well as the mast-cell count in the mesentery appeared between 1 and 2 weeks after adjuvant injection. This period approximately coincided with that of the development of secondary inflammatory lesions such as polyarthritis. The adjuvant-induced changes in the peritoneal fluid were more marked in SD than in Wistar rats, corresponding to more intense adjuvant arthritis in the SD strain. In Wistar rats a tendency towards an increase in the histamine content of the mesentery was observed from 2 weeks after adjuvant injection. Adjuvant injection did not have any significant effect on the histamine content of the caecum or the skin of either strain. These results show that adjuvant causes a redistribution of mast cells between the peritoneal fluid and the tissues surrounding the peritoneal cavity probably as a result of delayed hypersensitivity.

Animals↗
PubMed1980

Decrease in peritoneal mast cell count in rats with adjuvant arthritis. II. Inhibition by anti-inflammatory and immunosuppressive drugs.

The following drugs were administered daily to adjuvant-injected rats: indomethacin, flufenamic acid, phenylbutazone, prednisolone, dexamethasone, methotrexate and 6-mercaptopurine. The effects on the decrease in the mast-cell count and histamine content of the peritoneal fluid were studied as well as the effect on the development of polyarthritis 14 days after adjuvant injection. At the doses tested, these drugs markedly inhibited changes in the parameters of peritoneal fluid. With the exception of flufenamic acid, the preventive effects on the decrease in the peritoneal mast-cell count paralleled the prevention of development of arthritic lesions. 6-Mercaptopurine administered daily for 5 consecutive days starting from day 0 was almost as effective as when it was given over the entire period (days 0 through 13). These results show that the decrease in the peritoneal mast-cell count is one of the symptoms of adjuvant disease in rats that can be used for the quantitative evaluation of antirheumatic drugs.

Animals↗
PubMed1980

Handy type haemofiltration-plasma exchange apparatus.

A simple, compact and light weight design for a machine to perform plasma exchange or haemofiltration at a desired place is required. Based on these considerations, we have designed and fabricated a portable machine having a unique volume balancing mechanism and evaluated the in vitro and in vivo fluid balancing performances. We have obtained fully acceptable results during the evaluations and have performed clinical tests with favourable results.

Blood↗
PubMed1979

Effects of BCG, levamisole and PS-K on the rejection of male skin grafts by female mice.

Rejection of male skin grafts by BALB/c female mice was accelerated by one s.c. injection of BCG (5 X 10(5) microorganisms/mouse) into recipients on the day of transplantation. Levamisole 20 mg/kg injected similarly was without any effect. Protein-bound polysaccharide Kureha (PS-K) injected 250 mg/kg s.c. or i.p. once every other day from the day of transplantation stimulated graft rejection. The s.c. route was more effective than the i.p. route. These results show that, in sex-linked graft rejection in mice, PS-K has an immunostimulant action similar to that of BCG. This property may be important to the antineoplastic activity of PS-K.

Animals↗
PubMedDate not supplied

Age-related changes of bile acid metabolism in rats.

Cholesterol and bile acid leves were examined in young (8 weeks), middle-aged (12 months) and old (24 months) germ-free male rats, and young (8 weeks) and middle-aged (12 months) conventional male rats. The plasma cholesterol levels were higher in the aged rats, being more marked in the conventional rats. The liver cholesterol levels also increased with age and the increases were almost identical for both groups. No age-related changes were found in the biliary bile acid secretion, the pool size and distribution of bile acids in the bile, small intestine and large intestine, nor in the turnover frequency of bile acids, but the pool size in the young and middle-aged germ-free rats was much larger than that in the conventional rats. The turnover frequency was less in the germ-free rats. The bile acid synthesis presumed from the fecal bile acid excretion decreased in the aged germ-free rats but not in the conventional rats. A most remarkable age-related change was found in the bile acid composition; cholic acid increased and beta-muricholic acid derived from chenodeoxycholic acid in the rat decreased by aging, resulting in an increase of the CA/CDCA ratio (bile acids belonging to the cholic acid group/bile acids to the chenodeoxycholic acid group) in the bile, feces and pool. These results suggest that cholic acid synthesis increases while chenodeoxycholic acid synthesis is impaired by aging in rats.

Journal Article↗