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Biomedical subjects

S Sekine

Publications and source records attributed to S Sekine.

At least 109 records · Page 6Linked to original sources
PubMed1990

[Long-term results of total correction of tetralogy of Fallot less than 2 years old].

Between April 1972 and March 1979, eighteen total correction for tetralogy of Fallot less than 2 years old were performed with a mortality rate of 11.1%. The valved patch made of autologous pericardium was utilized for right ventricular outflow reconstruction in 12 of 16 survivors. Late (from 10 to 17 postoperative year) results of the all survivors were studied on functional and clinical status, hemodynamics and cardiac functions, and also, were compared with that of the patients above 2 years of age. All of them were in NYHA class I with no physical exercise limitation. Chest X-ray showed nearly normal CTR of 51.7%. ECG showed complete or incomplete RBBB in 14 patients and LAD in 3 of them, whereas no ventricular arrhythmia. Cardiac catheterization and cineangiography revealed that they had quite satisfactory hemodynamics and cardiac functions despite their moderate pulmonary regurgitation. In comparison with the older patient group, this younger one had a tendency to take superior results in all parameters of cardiac functions and clinical status. These excellent long term results seem to come from their young and less degenerative myocardium at the time of surgery.

Adolescent↗
PubMed1990

[Surgical treatment of impending rupture of aortic arch aneurysm associated with ischemic heart disease].

A 58-year-old man with thoracic aneurysm was admitted because of progressive chest oppression, back pain and the rapidly grown mediastinal mass lesion in his chest X-ray. Aortography and computed tomography revealed impending rupture of saccular aortic arch aneurysm. Emergency operation, consisted of aortic arch replacement under selective cerebral perfusion and CABG with the left IMA, was performed successfully. His postoperative course was uneventful without any neurological deficit. Postoperative angiography demonstrated successful reconstruction of the aortic arch and the patent IMA graft.

Aorta, Thoracic↗
PubMed1990

[Clinical study on the use of retrograde cardioplegia with St. Thomas' Hospital solution].

One hundred and three consecutive patients with aortic valve disease and twenty seven patients with ischemic heart disease of severe critical coronary stenosis or left main trunk stenosis underwent open heart operations with the use of retrograde cardioplegic technique for myocardial protection. Under complete cardiopulmonary bypass, a balloon catheter was inserted into the coronary sinus through small right atriotomy and secured in place. Retrograde cardioplegia was accomplished using cold St. Thomas' Hospital solution by drip method at height of 60 to 80 cm with topical saline slush. Cardiac resuscitation was very easy and acceptable hemodynamics were obtained in all patients. Even in 8 patients in which aortic crossclamping time was above 180 minutes cardiac recovery was excellent except one who needed IABP support. Eight patients in aortotomy group were died postoperatively from the reasons unrelated to myocardial protection. Postoperative hemodynamic data and enzymatic analyses of CK-MB revealed good myocardial protective effects. Retrograde cardioplegia with the use of cold St. Thomas' Hospital solution is thus an effective alternative of myocardial protection in aortic valve surgery or aortotomy surgery and in coronary revascularization for multiple coronary stenoses or left main trunk lesions.

Adult↗
PubMed1990

[Coronary revascularization using an internal mammary artery-saphenous vein composite graft in a patient with severely calcified ascending aorta].

A 52-year-old woman with angina pectoris resulted from complete obstruction of left coronary ostium required double-vessel bypass. Because of severely calcified ascending aorta, avoidance of aortic cross-clamping was needed for the prevention of embolic injury and aortic dissection. Internal mammary artery (IMA)-saphenous vein (SV) composite graft under hypothermic ventricular fibrillation was successfully performed without any complication. IMA-SV composite graft is a good alternative in a case of insufficient IMA length and limited site for proximal vein graft anastomosis, which can avoid or reduce the manipulation of diseased ascending aorta.

Aortic Diseases↗
PubMed1989

Isolation and characterization of a novel growth hormone cDNA from chum salmon (Oncorhynchus keta).

A cDNA clone which codes for a novel growth hormone has been isolated from the library of chum salmon pituitaries. The clone encodes a polypeptide of 210 amino-acid residues including 22 amino-acid residues of signal peptide, which is identical in length with known chum salmon growth hormone. In the coding region, there are 30 base substitutions, some of which result in 12 amino-acid substitutions. There are 8 base changes in the 5' untranslated region, and large insertions/deletions are in the 3' non-coding region. These results clearly indicate that there are at least two species of mRNAs for growth hormone in chum salmon pituitary.

Amino Acid Sequence↗
PubMed1989

Mutagenesis of human granulocyte colony stimulating factor.

To define the structure-function relationship, we have made a number of mutants of human granulocyte colony-stimulating factor (hG-CSF) by in vitro mutagenesis. The results indicate that most of the mutations located in the internal and C-terminal regions of the molecule abolished the activity, whereas the mutants without N-terminal 4, 5, 7, or 11 amino acids retained the activity. N-terminal amino acids were also altered by cassette mutagenesis using a synthetic oligonucleotide mixture. Among them, KW2228, in which Thr-1, Leu-3, Gly-4, Pro-5 and Cys-17 were respectively substituted with Ala, Thr, Tyr, Arg and Ser, showed more potent granulopoietic activity than that of intact hG-CSF both in vitro and in vivo.

Amino Acid Sequence↗
PubMed1989

Molecular cloning and sequence analysis of chum salmon gonadotropin cDNAs.

cDNAs encoding alpha and beta subunits of salmon gonadotropins, sGTHI and sGTHII, have been isolated from the cDNA library prepared from salmon pituitary mRNA. sGTHI alpha, sGTHI beta, and sGTHII beta cDNAs encode polypeptides of 114, 137, and 142 amino acids, including signal peptides of 22, 24, and 23 amino acids, respectively. The deduced amino acid sequence for sGTHI alpha revealed rather high homology (66-69%) to mammalian alpha chains, whereas sGTHI beta and sGTHII beta show lower homology (30%) to each other and to mammalian beta subunits. The existence of two distinct beta-subunit cDNAs in the teleost suggests that divergence of the GTH gene took place earlier than divergence of teleosts from the main line of evolution leading to tetrapods.

Amino Acid Sequence↗
PubMed1989

Outbreak of herpangina associated with Coxsackievirus B3 infection.

An outbreak of acute febrile illness was observed in summer, 1987, in a welfare home in which 31 healthy infants were accommodated. Within a 5-day period 25 infants (81%) acquired a febrile illness. Coxsackievirus B3 was isolated from 16 (64%) of 25 throat swabs. In the patients in whom viral culture was negative or not performed, 6 were serologically identified as having a coxsackievirus B3 infection. Among 22 patients identified as having a coxsackievirus B3 infection 7 had typical herpangina and the others had pharyngitis with or without a few small vesicles. Serum alpha-interferon was detected in all but 2 cases (one with proved infection and another with indefinite infection). Herpangina can be associated with coxsackievirus B3 as well as with the more frequently associated coxsackievirus Group A; this explosive type of outbreak might be transmitted by a small particle aerosol.

Antibodies, Viral↗
PubMed1989

Prevalence of small round structured virus infections in acute gastroenteritis outbreaks in Tokyo.

During the three-year period from 1984 to 1987, 506 acute gastroenteritis outbreaks involving 14,383 patients were reported to the Bureau of Public Health, Tokyo Metropolitan Government. Eighty (4,324 patients) of 150 outbreaks (4,860 patients) from which etiologic agents were not identified were subjected to virological investigation. Spherical particles of 28-32 nm in diameter with capsomere-like structures on the surface were detected in patients' stool specimens. Buoyant density of the particles appeared to be 1.36 to 1.40 g/ml in CsCl. Seroconversion to the particles was observed in patients by immune electron microscopy. From these observations, we concluded that the detected particles were members of small round structured virus (SRSV), and that they were implicated in the etiologically ill-defined outbreaks encountered. Prevalence of SRSV infections in these outbreaks was examined by electron microscopy. SRSV was positive in 83.8% of the outbreaks, and 96.4% of the cases. SRSV-positive outbreaks usually occurred during winter in contrast to bacterial outbreaks which often occurred in the summer season. Of 80 outbreaks examined, 53 were associated with the ingestion of oysters, and the remaining 27 mostly with food other than oysters. Oyster-associated outbreaks usually occurred on a small scale, while unassociated ones on diverse scales ranged from family clusters to large outbreaks.

Acute Disease↗
PubMed1989

Western blot (immunoblot) assay of small, round-structured virus associated with an acute gastroenteritis outbreak in Tokyo.

Small, round-structured virus (SRSV) was detected in a stool specimen of a patient during an acute gastroenteritis outbreak in Tokyo and was tentatively named SRSV-9. SRSV-9 was purified by sucrose velocity gradient centrifugation after CsCl density gradient centrifugation. The buoyant density of SRSV-9 appeared to be 1.36 g/ml in CsCl. A Western blot (immunoblot) assay using the biotin-avidin system revealed that SRSV-9 was antigenically related to the Hawaii agent but distinct from the Norwalk agent and contained a single major structural protein with a molecular size of 63.0 +/- 0.6 kilodaltons. The prevalence of SRSV-9 infection in Tokyo was surveyed by the Western blot antibody assay by using a crude virus preparation as the antigen. Seroconversion was observed in 56.5% of the patients involved in the outbreaks from which SRSV was detected by electron microscopy.

Acute Disease↗
PubMed1989

[Transseptal suture fixation in the management of prosthetic valve dehiscence].

Valve dehiscence resulted from prosthetic valve endocarditis and aortitis is the most common cause of the reoperation after prosthetic valve replacement. In our experience, the results of reoperation has been far from satisfactory. For the successful management of prosthetic dehiscence, we have adopted the transseptal suture technique of accurately securing the prosthetic valve. Five patients with prosthetic valve dehiscence and one patient with native valve endocarditis were operated upon using the transseptal techniques. Left ventricular-aortic discontinuity was repaired using composite valve-tube graft together with this technique in two patients. All but one patient who succumbed to multiple organ failure induced by preoperative hemodynamic deterioration have survived the operation without recurrent infective endocarditis or valve dehiscence.

Adult↗
PubMed1988

Molecular cloning of eel growth hormone cDNA and its expression in Escherichia coli.

cDNA clones coding for growth hormone (eGH) of Japanese eel (Anguilla japonica) have been isolated from a cDNA library prepared from pituitary gland poly(A)+ RNA. The nucleotide sequence of the eGH cDNA was determined. It codes for the prehormone of 209 amino acids (aa) including a putative signal peptide of 19 aa. The deduced amino acid sequence was identical with that determined for eGH protein. The primary structure of eGH was compared with those of other species growth hormones (chum salmon, chicken, rat, and human). Mature eGH was expressed in Escherichia coli harboring a plasmid in which the eGH cDNA was under control of the phage lambda pL promoter. Recombinant eGH polypeptide was immunoreactive to rabbit antiserum against natural eGH. Furthermore, eGH derivative with amino-terminal deletion (delta 1-3 eGH) was produced in E. coli reaching up to 5% of total cellular proteins.

Amino Acid Sequence↗
PubMed1988

A gag-env hybrid protein of human T-cell leukemia virus type I and its application to serum diagnosis.

A fused gene of the gag and env sequences of human T-cell leukemia virus type I (HTLV-I), the causative agent of adult T-cell leukemia, was constructed in vitro and expressed in Escherichia coli. The gag-env hybrid protein accumulated as insoluble granules with a yield of approximately 12% of the total proteins. In an enzyme-linked immunosorbent assay done with the use of the gag-env hybrid protein, all 57 seropositive sera gave positive signals, and none of the sera from normal persons did. This system can produce large quantities of the gag-env hybrid protein, which can be used for mass screening of human sera for HTLV-I infection.

Epitopes↗
PubMed1988

[Enhancement of antitumor effect of MMC on Walker 256 by intra-arterial administration of noradrenaline in hyperthermia].

We performed a study of hyperthermia while injecting 0.05% of Noradrenaline following MMC for 10 minutes into the feeding artery of Walker-256 carcinosarcomas implanted 6 days earlier into the s.c. dorsum side of hindpaw of Wistar rats. The tumor growth rates on the 6th day after treatment by warning tumor in hot water (40 degrees C, 44 degrees C) for 10 minutes with or without Noradrenaline, were 0.7 +/- 0.6, 2.1 +/- 0.9 (40 degrees C) and 0.2 +/- 0.3, 0.0 +/- 0.0 (44 degrees C), respectively. The data suggested that tumor ischemia induced by a vasoconstrictive drug may enhance the antitumor effect in low grade warning therapy (40 degrees C). An injection of warmed physiological saline (50 degrees C) may heat the tumor vessels on the tumor surface and showed enhanced antitumor effects as a from of hyperthermia. The target area of the tumor for hyperthermia can be considered to be the tumor vessels on the tumor surface.

Animals↗