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S Seidl

Publications and source records attributed to S Seidl.

154 records · Page 9Linked to original sources
PubMedDate not supplied

Ethyl glucuronide--a marker of alcohol consumption and a relapse marker with clinical and forensic implications.

Ethyl glucuronide (EtG) is a non-volatile, water-soluble, direct metabolite of ethanol that can be detected in body fluids and hair. We investigated urine and serum samples from three patient groups: (1) 33 in-patients in acute alcohol withdrawal; (2) 30 detoxified in-patients (treated for at least 4 weeks) from a 'motivation station'; and (3) 43 neuro-rehabilitation patients (non-alcoholics; most of them suffering from stroke, traumatic brain injury, Parkinson's disease etc.) using gas chromatography/mass spectrometry (GC/MS) with deuterium-labelled EtG as the internal standard and additionally in the second group of patients using liquid chromatography (LC/MS-MS). We found no correlation between the concentration of EtG in urine at hospitalization and the blood-ethanol concentration (r = 0.17), the time frame of detection (r = 0.5) or the total amount of clomethiazole required for the treatment of withdrawal symptoms (r = 0.28). In four out of 30 in-patients from the 'motivation station'--where neither clinical impression nor routine laboratory findings gave indications of relapse--concentrations of EtG in urine ranged between 4.2 and 196.6 mg/l. EtG concentrations in urine of between 2.89 and 23.49 mg/l were found in seven out of 43 neuro-rehabilitation patients using GC/MS. The GC/MS and the LC/MS-MS results showed a correlation of 0.98 with Pearson's correlation test and 1.0 with Spearman's correlation test. We suggest that EtG is a marker of alcohol consumption that can be detected for an extended time period after the complete elimination of alcohol from the body. When used as a relapse marker with a specific time frame of detection intermediate between short- and long-term markers, EtG fills a clinically as well as forensically important gap. Its specificity and sensitivity exceed those of all other known ethanol markers.

Adult↗
PubMedDate not supplied

The role of formaldehyde in the formation of haemodialysis-associated anti-N-like antibodies.

In the sera of 68 (20.9%) of 325 haemodialysis patients, a specific cold agglutinin, cross-reacting with anti-N, was detected. Each patient showing this anti-N-like antibody used a formaldehyde-sterilized dialyser. By contrast, the antibody was not found in any of the 73 patients using a non-formaldehyde-sterilized dialyser. The results of transfusion experiments indicate an in vivo haemolytic activity of the antibody. In vitro, formaldehyde pretreatment of erythrocytes increased the agglutinability of the cells by the anti-N-like antibody. This effect could be prevented when the MN receptor of the red cells was exposed to neuraminidase. Trace quantities of formaldehyde inevitably enter the patient during dialysis when a formaldehyde-sterilized dialyser is used. It is postulated that this in vivo formaldehyde exposure might interact with the MN receptor of the red cells, rendering it immunogenic, and thus induce the formation of the haemodialysis-associated anti-N-like antibody.

Antibody Specificity↗
PubMedDate not supplied

[Suicide by guillotine].

The authors describe the suicide of an 21 year old man, who executed himself with a self-constructed "guillotine". In spite of his serious neck wound he was able to deliver himself from the guillotine. He died due to severe haemorrhage from the right carotid artery damaged by the cut.

Adult↗