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Biomedical subjects

S Sawano

Publications and source records attributed to S Sawano.

At least 37 records · Page 2Linked to original sources
PubMed1990

[Studies on immunoreactive somatostatin and gastrin contents of the gastric mucosa in patients with duodenal ulcer--comparison to patients with fundic gland polyposis and normal subjects].

The immunoreactive gastrin (IRG) and somatostatin (IRS) contents in gastric mucosa were measured from the same biopsy specimen of the same patients with duodenal ulcer (DU) at the active stage and healing stage, and compared to those of patients with fundic gland polyposis (FP) and endoscopically normal subjects whose gastric mucosa had only slight atrophic change (Control). The IRS in both the antrum and the gastric body of DU were significantly lower than those of the other two groups, and those showed no difference between the two stages. In all groups, there was a significant positive relation between the IRG and IRS in the antrum. In DU, particularly at the active stage, the relative decrease of the IRS against the IRG was prominent compared to the other two groups. In FP, which has similar background gastric mucosa and ability of acid output to those of DU, it was found that somatostatin was secreted sufficient to control gastrin secretion and acid output. Whereas in DU, secretion of somatostatin was reduced and, particularly at the active stage, it was considered that somatostatin, which could control increased gastrin secretion and increased acid output, was not secreted.

Adult↗
PubMed1989

ACTH determination in a petrosal sinus venous specimen after corticotrophin releasing factor provides the best clue on the laterality of microadenoma in Cushing's disease.

A 42-year-old woman with Cushing's disease was endocrinologically cured after transsphenoidal selective excision of a left-sided microadenoma. Simultaneous samplings from the bilateral inferior petrosal sinuses with ovine corticotrophin-releasing factor (CRF) stimulation were performed preoperatively to evaluate some relations between the localization of a possible microadenoma and the levels of ACTH in inferior petrosal sinuses. The data for the venous samplings were as follows:-(1) The basal levels of ACTH obtained simultaneously from both inferior petrosal sinuses and peripheral vein were about the same. (2) A significant difference, with a high level of ACTH from the ipsilateral side of the microadenoma, was demonstrated after CRF stimulation. (3) The magnitude of increase in ACTH on the contralateral side after CRF was similar to that of the peripheral response. It is recommended that all patients with ACTH dependent Cushing's syndrome and negative radiological findings should have bilateral simultaneous inferior petrosal venous sampling with CRF stimulation.

Adenoma↗
PubMed1984

Growth hormone response to human pancreatic growth hormone releasing factor in cattle.

The effects of a growth hormone releasing factor, human pancreatic growth hormone releasing factor-44 (hpGRF-44), on growth hormone (GH) secretion in calves, heifers and cows were studied. A single intravenous (iv) injection of 0.1, 0.25, 0.5 or 1.0 microgram of synthetic hpGRF-44 per kg of body weight (bw) in calves significantly elevated the circulating GH level within 2-5 min, while no increase in plasma GH was observed in saline injected control calves. The plasma GH level increased proportionally to the log dose of hpGRF-44, and reached a peak at 5-10 min (p less than 0.01). Subcutaneous injection of hpGRF-44 also elevated the plasma GH level, but the peak value at 15 min was 37% of that of iv injection (p less than 0.05). Intravenous injection of 0.25 microgram of hpGRF-44 per kg of bw to female calves, heifers, and cows significantly elevated mean the GH levels from 8.5, 2.3, and 1.6 ng/ml at 0 time to peak values of 97, 26, and 11.6 ng/ml, respectively (p less than 0.01). The plasma GH response and basal level in calves were significantly higher than those of heifers or cows (p less than 0.025). The plasma GH response to hpGRF-44 as well as the basal level decreased with advancing age. The plasma GH response to hpGRF-44 and basal GH in male calves were significantly greater than those in female calves (p less than 0.001). These results indicate that synthetic hpGRF-44 is a potent secretogogue for bovine GH, and suggest its usefulness in the assessment of GH secretion and reserve in cattle.

Animals↗
PubMed1983

Islet-cell antibodies in IDDM and NIDDM in a Japanese population.

Islet-cell antibodies (ICA) were studied in 538 Japanese diabetics with insulin-dependent diabetes mellitus (IDDM) and non-insulin-dependent diabetes mellitus (NIDDM). The overall prevalence of ICA was 17% (16/93), 4% (7/164), 2% (2/90) and 3% (5/191) in IDDM, NIDDM treated with insulin, NIDDM treated with oral hypoglycemic agents, and NIDDM treated by diet alone, respectively. ICA were detected in 58% (11/19) of patients with IDDM in the first year of the disease. The prevalence of ICA decreased drastically with prolongation of the disease. We could not find any ICA-positive IDDM in subjects with a clinical course of more than 10 years. The level of thyroid microsomal antibodies was not significantly higher in either type of diabetes when compared to nondiabetic controls. These results suggest that Japanese diabetics may have a weaker autoimmune predisposition than Caucasians.

Adult↗
PubMed1983

The pharmacokinetics of insulin after continuous subcutaneous infusion or bolus subcutaneous injection in diabetic patients.

Pharmacokinetic models of insulin were examined in order to describe a plasma concentration-time profile after subcutaneous (s.c.) administration of insulin to the patients with insulin-dependent diabetes mellitus (IDDM) or non-insulin-dependent diabetes mellitus (NIDDM). Diabetic subjects were restricted to those with fasting plasma insulin levels around the lowest limit for insulin assay (5 microU/ml). A one-compartment open model with first-order absorption and elimination was appropriate for estimating the plasma concentration-time profile of insulin injected or infused subcutaneously. In the case of continuous s.c. insulin infusion (CSII) for 1 h at the rate of 3 ml/h (2--3 U/ml), the absorption rate constant (Ka), elimination rate constant (Ke), and distribution volume (Vd) were 0.026 +/- 0.001 min-1 (mean +/- SEM; absorption half-life: 27 min), 0.013 +/- 0.005 min-1 (elimination half-life: 53 min), and 1.99 +/- 0.49 L/kg body wt, respectively. These values did not differ significantly from those generated by single bolus s.c. injection of undiluted insulin (40 U/ml). The calculated areas under the plasma insulin concentration-time curves from time zero to infinity ([AUC] 0 infinity) did not differ after each mode of administration, while the [AUC] 0 infinity after CSII was about 32% of that following intravenous bolus injection (P less than 0.01). The following conclusions can be drawn from these results: (1) the plasma concentration-time profile of insulin after CSII or bolus s.c. injection can be analyzed by pharmacokinetic modeling, (2) the absorption kinetics of insulin did ot differ significantly between two modes of s.c. insulin administration in the patients with IDDM or NIDDM, and (3) the insulin after CSII or single bolus s.c. injection seems to be degraded at the s.c. site to the same extent.

Adult↗
PubMed1981

The prevalence of islet-cell antibodies and complement-fixing islet-cell antibodies in Japanese diabetics.

The presence of complement-fixing islet-cell antibodies (CF-ICA) and islet-cell antibodies (ICA) was examined in 355 patients with different types of diabetes mellitus in the Japanese population by an indirect immunofluorescence test (IFT). The overall prevalence of ICA, which were stained as a homogenous cytoplasmic fluorescence in islet cells, was 7 per cent (5/67) in insulin-dependent (Type I) diabetics, 4 per cent (6/137) in noninsulin-dependent (Type II) diabetics treated with insulin and 2 per cent (1/58) in Type II diabetics treated with oral hypoglycemic agents. None of 84 Type II diabetics receiving diet alone and 9 diabetics associated with chronic pancreatitis had ICA. CF-ICA, which were stained as a "ring-shaped" fluorescence in a part of the cytoplasma, were demonstrated in 5 out of 12 cases (42%) whose sera possessed ICA. The lower prevalence and remarkably shorter persistence of ICA and CF-ICA in Japanese diabetics than those observed in Caucasian diabetics may be explained by the heterogenous immunological response in different races or possible heterogeneity of Type I diabetics.

Adolescent↗
PubMed1981

Effect of theophylline on insulin, glucagon and somatostatin secretion from the isolated rat pancreatico-duodenal preparation.

In order to determine whether endogenous somatostatin has an inhibitory action on insulin and glucagon secretion, the isolated rat pancreas was perfused with 10mM theophylline, which strongly stimulated somatostatin release, under pretreatment or simultaneous administration of 8.3, 16.7 mM glucose and 20mM arginine with 4.4 mM glucose. 1) In the infusion of 10mM theophylline with 8.3 mM glucose, the first phase at the time corresponding with that of insulin release elicited by 8.3 mM glucose alone was suppressed by endogenous somatostatin stimulated by theophylline. 2) During the infusion of 16.7 mM glucose for 35 min, 10mM theophylline was interposed at from 15 to 25 min intervals. Its addition caused a rapid increase in somatostatin. Only at 15 min 30 sec and 16 min, insulin concentration went down and reached the levels of 70 and 74 per cent of the 15-min value, respectively. 3) During the infusion of 20 mM arginine with 4.4 mM glucose for 35 min, 10mM theophylline was added to the perfusate from 15 to 25 min. The addition of theophylline caused an increase in somatostatin and decrease in glucagon. At 15 min 30sec and 16 min, the levels of glucagon were 63 and 75 per cent of 15-min value, respectively. These results seem to support the idea that endogenous somatostatin suppressed glucose-induced insulin and arginine-induced glucagon secretion. However, the possibility of a direct effect of theophylline on the changes in insulin and glucagon secretion could not be excluded.

Animals↗