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S S Elberg

Publications and source records attributed to S S Elberg.

32 records · Page 2Linked to original sources
PubMed1970

Macrophage membranes viewed through a scanning electron microscope.

When rabbit peritoneal macrophages were cultured in serum and Tyrode medium, numerous membranous structures varying in size and transparency were observed to cover the surface in complex cascading patterns. Modifications in the methods of fixation and drying probably accounted for the new perspective of macrophage surfaces gained in this study.

Animals↗
PubMed1969

Serum-mediated immune cellular responses to Brucella melitensis. IV. Infection of macrophages under anaerobic conditions.

Immune mechanisms active against Brucella were studied under conditions of oxygen deficiency. B. melitensis grew in rabbit serum-Tyrode medium flooded with N(2) and CO(2) gas mixtures. Immune sera from rabbits injected with B. melitensis strain Rev I possessed growth-inhibitory activity that operated in anaerobic environments against Rev I and virulent strain 6015. When mixed with macrophages, immune sera mediated even greater inhibition of bacterial growth and slowed the spread of infection throughout the tissue culture. Although under anaerobic conditions the rate of phagocytosis was reduced, the macrophages in immune serum killed significant percentages of Brucella, suggesting that an antibacterial mechanism had been activated. Sonic extracts of macrophages prepared and tested under anaerobic conditions depressed the growth rate of strain Rev I. The extracts, however, exhibited no immediate killing capacity when tested in Tyrode solution. A factor from serum was required for depression of the growth rate.

Animals↗
PubMed1968

Serum-mediated immune cellular responses to Brucella melitensis. I. Role of a macrophage-stimulating factor in promoting ingestion of Brucella by streptomycin-protected cells.

Injection of rabbits with living Brucella melitensis Rev I induced the appearance of a macrophage-stimulating-factor (MSF) in the sera of these animals. MSF was involved in ingestion of bacilli, hastening the formation of protected loci as measured by the addition of lethal amounts of dihydrostreptomycin. When sufficient time had been allowed for effective ingestion, streptomycin had little effect. This in turn allowed for multiplication of bacilli intracellularly in the presence of 5 to 250 mug of drug per ml. MSF mediated more effective ingestion by both immune and normal macrophages. Under such conditions, there was little, if any, intracellular growth restriction by macrophages from immune animals. The activity appeared within the first 5 days after injection with 10(9) organisms and was present for several months. Three weeks after injection, the activity of serum was partially heat-labile. All activity was removed by absorption with heat-killed or living Rev I cells, suggesting that a specific globulin is concerned.

Blood↗