Biomedical subjects

S Ruf

Publications and source records attributed to S Ruf.

41 records · Page 3Linked to original sources

PubMed1994

Complete RNA editing of unspliced and dicistronic transcripts of the intron-containing reading frame IRF170 from maize chloroplasts.

The maize plastome harbors within the rps4-rps14 gene cluster the reading frame IRF170, which is interrupted by two introns. Although the function of the encoded peptide of 170 amino acids is not known, the conservation of IRF170 homologs in other plastomes is a strong indication that IRF170 is a functional gene. Amplification and sequence analyses of IRF170 specific cDNAs reveals two C-to-U editing events occurring within each of the first two exons. This situation allows an analysis of the temporal order between editing and splicing of a chloroplast transcript. By using intron-specific primer combinations, cDNAs derived from partially or even unspliced IRF170 transcripts could be amplified which in all cases showed complete editing. Complete editing was also observed with a cDNA derived from a transcript in which the proximal rps4 and the 5' half of IRF170-encoded sequences were still linked. This demonstrates that editing of the IRF170 transcript is an early processing step preceding both splicing and cleavage to monocistronic mRNA.

Amino Acid Sequence↗

PubMed1994

[Facial morphology and the size and activity of the masseter muscle].

The study presented here was conducted for the purpose of investigating the possible relationship of masseter muscle size and activity to facial morphology. The study examined 50 adults, 25 males and 25 females, by means of electromyography, ultrasonography, and facial photography. The following results were obtained. There was considerable variation in masseter muscle activity and size both between the 2 groups and among individuals in each group. Partly there were strong differentiation's between the males and the females. The interrelationships between masseter muscle activity and size and facial morphology in both groups were generally weak, however, the links were more discernible in the women than in the men. Female subjects with thin faces and large mandibular planes had reduced masseter thickness, whereas women with a small anterior facial height index displayed when relaxed less masseter muscle activity and a smaller masseter width.

Adult↗

PubMed1988

Significance of serum phosphohexose isomerase in gastrointestinal cancer at different stages.

The purpose of this study was to reevaluate the significance of serum PHI in gastrointestinal cancer at histopathologically defined stages prior to primary treatment. A total of 248 patients with malignant tumors of the gastrointestinal tract and a collective of 42 patients with noncancerous diseases were studied. The results are compared with those obtained with the established markers tissue polypeptide antigen (TPA) and carcinoembryonic antigen (CEA). Phosphohexose isomerase (PHI) revealed an overall diagnostic sensitivity of 69%, combined with a specificity of 74%. The corresponding data for TPA were found to be 73 and 47% while for CEA 26 and 95% respectively were determined. Even in the early stages of colorectal and esophageal carcinoma, PHI showed a sensitivity of about 60%. A continuous rise of PHI serum levels, correlating well with the extent of the tumor disease, could be detected. In contrast to TPA and CEA, PHI assay can be carried out with a minimum of laboratory efforts, in a short time and at low costs. These findings suggest that serum PHI assay is a useful aid for screening of gastrointestinal cancer, especially esophageal and gastric carcinoma, and a reliable marker for treatment control and follow-up.

Antigens, Neoplasm↗

PubMed1987

Avoiding early failure of tibial prosthetic bypass grafts.

In a series of 97 tibial reconstructions using PTFE bypass grafts the value of perioperative anticoagulation was studied. Acetylsalicylic acid as well as low-dose heparin treatment resulted in a high graft failure rate during the first postoperative days (42%). Patency could be restored in half of the cases by simple thrombectomy. When heparin was given at a dose of 1000 I.U. per hour for two days the graft failure rate was 14%. Only one out of eight thrombosed grafts could be re-opened by thrombectomy. Long-term patency, however, was similar in both groups. We conclude that perioperative heparin treatment is a suitable method to avoid unnecessary thrombectomies of long prosthetic bypass grafts.

Blood Vessel Prosthesis↗

PubMed1977

[Postoperative control of blood flow in arterial surgery: results of electromagnetic blood flow measurement (author's transl)].

An electromagnetic blood flow cuff probe was implanted up to 4 days in 50 patients to control basal blood flow through a femoro-popliteal vein graft. Patent grafts showed increasing blood flow due to hyperemia with maximum on the 2. p. op. day. The level of the postoperative hyperemia and the increase of blood flow caused by intraarterial papaverine injection during operation were in the same range. The method and the extension of analgesia influenced the degree of postoperative hyperemia. Pain and blood loss diminuished graft flow. Patients with epidural block and sufficient blood volume produced a significant higher graft flow than patients with morphine analgesia. 2 patients had early occlusion of the graft, 2 others showed occlusion at the distal anastomosis. These complications could be easily detected by blood flow measurement and were successfully corrected in two patients. Moreover, electromagnetic postoperative blood flow control may be helpful in patients with proximal a.v. shunt for hemodialysis.

Arterial Occlusive Diseases↗