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S Rottem

Publications and source records attributed to S Rottem.

182 records · Page 11Linked to original sources
PubMedDate not supplied

Binding of MVL-2 virus to A. laidlawii cells.

Binding of MVL-2 virus, whose envelope lipids were radioactively labeled, to A. laidlawii JA1 cells was determined and characterized. The binding followed first-order kinetics and was temperature-dependent. All MVL-2 particles were capable of binding to A. laidlawii cells. At least 75 percent of radioactive MVL-2 bound represented specific binding which was markedly inhibited by EDTA. Virus infectivity was not essential for binding as inactivation of the virus by ultraviolet irradiation did not affect binding. Nevertheless, protein denaturing agents or proteolytic enzymes markedly inhibited MVL-2 binding, suggesting that the binding site of MVL-2 is of proteinaceous nature.

Acholeplasma laidlawii↗
PubMedDate not supplied

Mycoplasma in the spotlight of AIDS: partial biochemical characterization of mycoplasma-derived components inducing TNF alpha secretion and blast transformation.

We have previously demonstrated that the AIDS-associated Mycoplasma fermentans as well as Mycoplasma capricolum membranes activated bone marrow macrophages to secrete tumor necrosis factor alpha (TNF alpha) and induce blast transformation of splenic lymphocytes. Herein, we show that the membrane component of Mycoplasma capricolum capable of inducing TNF alpha secretion is a hydrophobic protein. This is supported by our findings that the TNF alpha inducing activity was eluted by a phenyl-Sepharose column in a peak distinct from bulk membrane lipids. The hydrophobic nature of the protein is indicated by the activity of the "hydrophobic protein" fraction of the membranes, and the pattern of elution obtained by the phenyl-Sepharose column. Fractionation of the M. capricolum membranes, solubilized by CHAPS (3-[(3-cholamidopropyl)-dimethylammoniol]1-propane sulfate) on a gel filtration column revealed a major peak of TNF alpha inducing activity of about 75,000 daltons, and a minor peak of about 55,000 daltons. The mitogenic activity, though spread throughout the column, peaked in the same fractions as the TNF alpha inducing activity. Both activities co-eluted by the phenyl-Sepharose column as well. However, the mitogenic activity of the membranes was much more resistant to elevated temperatures and extreme pH treatment.

AIDS-Related Opportunistic Infections↗