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Biomedical subjects

S Rosipal

Publications and source records attributed to S Rosipal.

8 recordsLinked to original sources

[Lysosomal sphingomyelinase deficiency: spectrum of phenotypes in Czech and Slovak patients].

BACKGROUND: We present a series of 25 patients (from 21 families) with deficiency of lysosomal sphingomyelinase (acid sphingomyelinase, ASM), diagnosed during the last 30 years. METHODS AND RESULTS: Diagnosis was established by finding specific sphingomyelin storage pattern in bone marrow histiocytes and in some bioptical and postmortem tissues (presence of sphingomyelin liquid crystals) and finally by proving ASM deficiency in white blood cells and in cultured fibroblasts. Range of clinical manifestations of our patients notably exceeded the the known main (A,B) phenotypes described so far. In the group of type A patients (clinically overt neurovisceral symptomatology) there was significant tendency to prolonged course. Classical fulminant course with death between 5 to 45 months of age was seen only in a subgroup of 5 patients. In other type A patients (n = 8) the course was prolonged attaining 5 years of age, the end of the first (8, and 9 years), second (14, 17 years), third (22 years), fourth (32 years) and fifth decades (41 years). Three of these patients (aged 5, 22 and 41 years) are living. The series of patients with dominant visceral involvement (n = 12) consisted of three phenotypically different subgroups. One with chronic purely visceral affection and prolonged course (n = 4) corresponding to the classical type B, the second with chronic course and largely subclinical neurological affection (n = 4) and the third with accelerated fatal visceral affection (death in age range 31 months-9 years) without (n = 1) or with clinically minor signs of brain damage (n = 3). CONCLUSIONS: Study of the presented series of ASM deficient patients disclosed remarkable phenotypical variability. Two main factors seem to be responsible. Variability in the storage intensity in the two main tissue compartments (neuronal and visceral), and the absence of proportionality in their affection in some instances. The described phenotype variability enlarges significantly the known spectrum of phenotypes in ASM deficiency.

Child↗

[The fluorescence flow cytometry technique in the diagnosis of hereditary platelet disorders--a case of Glanzmann's thrombasthenia].

The authors describe a method for the diagnosis of heredital platelet membranopathies by means of monoclonal antibodies against the main membrane glycoproteins of thrombocytes, glycoprotein Ib and IIIa. The platelets are differentiated in the flow fluorocytometer from other blood cells by the typical optic profile caused by their size and granular character. Monoclonal antibodies are bound to the appropriate membrane glycoprotein and their amount is then detected by means of a secondary antibody labelled with fluorescein. The intensity of fluorescence of individual platelets is proportional to the number of molecules of the appropriate glycoprotein on their surface. By the above technique a case of Glanzmann's thrombasthenia was diagnosed, a rare hereditary haemorrhagic disease, characterized by the absence or abnormal function of glycoprotein complex IIb/IIIa the platelet receptor for fibrinogen.

Antibodies, Monoclonal↗

[Hereditary nephropathy with signs of nephrotic syndrome].

The authors submit the description of two sisters with hereditary nephropathy, with symptoms of nephrotic syndrome. In the clinical picture dominated permanent anuria, rapid renal failure and death at the end of neonatal age. Histopathological findings classified the disease as infantile diffuse mesangial sclerosis. The third patient is a sporadic case of primary congenital nephrotic syndrome.

Female↗

[Body growth in low birth weight premature children up to 5 years of age].

Investigation of the growth in 488 premature infants born with a low birth weight, compared with infants born in term with a birth weight above 2,500 g at the age of 1-5 years. Body weight, height, head circumference and chest circumference were assessed as well as Quetelet-Kaup-Gould and Quetelet-Bouchard index. It was found that premature infants are retarded in the above parameters, as compared with mature infants, still at the life of live years.

Anthropometry↗

[Leprechaunism].

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Abnormalities, Multiple↗