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S Reynolds

Publications and source records attributed to S Reynolds.

At least 55 records · Page 3Linked to original sources
PubMed1996

Effect of interferon alpha on HLA-DR expression by human buccal epithelial cells.

/We have studied the effect of interferon alpha (IFN-alpha) on MHC class II expression by human buccal epithelial cells (BEC), and mRNA expression by BEC and mucosal-associated mononuclear cells (MAMC). In 6 experiments, freshly collected BEC were suspended at a concentration of 1.0 x 10(5)/ml in RPMI 1640 and incubated in the presence of 0-10,000 IU/ml of human lymphoblastoid IFN-alpha (HuIFN-alpha). Zero and six hour samples were analyzed by single color flow cytometry using FITC-labeled murine IgG1 monoclonal antibody to HLA-DR. Preparations were also analyzed for expression of cytokine transcripts (IL-2, IL-4, IL-5, IL-6, IL-8, IFN-gamma, GM-CSF) by reverse transcriptase polymerase chain reaction (RT-PCR). Increasing concentrations of IFN-alpha resulted in proportionate increases in the percentage of HLA-DR + BEC (r = 0.7897, p = 0.0627) and in the percentage of HLA-DR+ staining at higher intensities (10(1) to 10(2) log fluorescence intensity) (LFI) (r = 0.4010, p = 0.0424). The percentage of HLA-DR + BEC rose from a mean of 1.5% with no IFN-alpha to 7% with 10,000 IU/ml IFN-alpha (p < 0.05). The percentage of HLA-DR + BEC staining at 10(1) to 10(2) LFI rose from a mean of 8.3% with no added IFN-alpha to 19.2% with 10,000 IU/ml IFN-alpha (p < 0.05). Unstimulated BEC constitutively expressed IL-8 and GM-CSF. IFN-alpha stimulated preparations also expressed IFN-gamma, possibly due to the presence of MAMC, which comprised 2-9% of the total cell population. These data indicated that HuIFN-alpha upregulates MHC class II expression by human BEC, possibly by enhancing IFN-gamma production by MAMC present in the culture preparations.

Cells, Cultured↗
PubMed1995

Effects of treatment duration and severity of depression on the maintenance of gains after cognitive-behavioral and psychodynamic-interpersonal psychotherapy.

One hundred four clients completed a mailed follow-up 1 year after completing 8 of 16 sessions of treatment, either cognitive-behavioral (CB) or psychodynamic-interpersonal (PI) psychotherapy. Although mean scores on outcome measures at 1 year suggested that gains were, in general, well maintained, only 29% of clients were asymptomatic on all 3 occasions of testing (end of treatment, 3 months and 1 year later) without recourse to further treatment. However, only 11% of those asymptomatic at end of treatment experienced relapse or recurrence of depression, albeit on the limited evidence of just two follow-up assessments. The results of comparisons among treatment conditions at 1 year differed substantially from those obtained earlier: Eight-session PI treatment now appeared less efficacious than the other 3 treatment conditions, and there was now no measurable benefit of 16-session over 8-session CB, irrespective of initial severity of depression. These findings confirm the importance of follow-up in evaluation of psychotherapies for depression.

Adult↗
PubMed1995

Impact of Cluster C personality disorders on outcomes of contrasting brief psychotherapies for depression.

Twenty-seven of 114 depressed clients, stratified for severity of depression, obtained a Diagnostic and Statistical Manual of Mental Disorders (3rd ed.; DSM-III; American Psychiatric Association, 1980) diagnosis of Cluster C personality disorder--that is, avoidant, obsessive-compulsive or dependent personality disorder (PD clients)--whereas the remaining 87 did not (non-personality-disorder [NPD] clients). All clients completed either 8 or 16 sessions of cognitive-behavioral (CB) or psychodynamic-interpersonal (PI) psychotherapy. On most measures, PD clients began with more severe symptomatology than NPD clients. Among those who received PI therapy, PD clients maintained this difference posttreatment and at 1-year follow-up. Among those who received CB therapy, posttreatment differences between PD and NPD groups were not significant. Treatment length did not influence outcome for PD clients. PD clients whose depression was also relatively severe showed significantly less improvement after treatment than either PD clients with less severe depression or NPD clients.

Adult↗
PubMed1995

Enhancing target variance in personality impressions: highlighting the person in person perception.

D. A. Kenny (1994) estimated the components of personality rating variance to be 15, 20, and 20% for target, rater, and relationship, respectively. To enhance trait variance and minimize rater variance, we designed a series of studies of personality perception in discussion groups (N = 79, 58, and 59). After completing a Big Five questionnaire, participants met 7 times in small groups. After Meetings 1 and 7, group members rated each other. By applying the Social Relations Model (D. A. Kenny and L. La Voie, 1984) to each Big Five dimension at each point in time, we were able to evaluate 6 rating effects as well as rating validity. Among the findings were that (a) target variance was the largest component (almost 30%), whereas rater variance was small (less than 11%); (b) rating validity improved significantly with acquaintance, although target variance did not; and (c) no reciprocity was found, but projection was significant for Agreeableness.

Adult↗
PubMed1995

Analysis of preventable trauma deaths and inappropriate trauma care in a rural state.

OBJECTIVE: The goal of this study was to determine the rate of preventable mortality and inappropriate care in cases of traumatic death occurring in a rural state. DESIGN: This is a retrospective case review. MATERIALS AND METHODS: Deaths attributed to mechanical trauma throughout the state and occurring between October 1, 1990 and September 30, 1991 were examined. All cases meeting inclusion criteria were reviewed by a multidisciplinary panel of physicians and nonphysicians representing the prehospital as well as hospital phases of care. Deaths were judged frankly preventable, possibly preventable, or nonpreventable. The care rendered in both preventable and nonpreventable cases was evaluated for appropriateness according to nationally accepted guidelines. MEASUREMENTS AND MAIN RESULTS: The overall preventable death rate was 13%. Among those patients treated at a hospital, the preventable death rate was 27%. The rate of inappropriate care was 33% overall and 60% in-hospital. The majority of inappropriate care occurred in the emergency department phase and was rendered by one or more members of the resuscitation team, including primary contact physicians and surgeons. Deficiencies were predominantly related to the management of the airway and chest injuries. CONCLUSIONS: The rural preventable death rate from trauma is not dissimilar to that found in urban areas before the implementation of a trauma care system. Inappropriate care rendered in the emergency department related to airway and chest injury management occurs at a high rate. This seems to be the major contributor to preventable trauma deaths in rural locations. Education of emergency department primary care providers in basic principles of stabilization and initial treatment may be the most cost-effective method of reducing preventable deaths in the rural setting.

Adolescent↗
PubMed1995

Adults in the pediatric emergency department: a fish out of water?

UNLABELLED: Adults (> 17 years) occasionally seek care in pediatric emergency departments (PED). We reviewed the records of adults treated in our PED from September 1992 to August 1993 to better understand their needs. Patients were classified based on their relationship to our hospital: 1) no previous medical care association (new), and 2) adult patients followed in our clinics for chronic diseases (chronic). Data were analyzed using frequencies and comparison of means by Student's t test or chi 2. A follow-up phone call or written survey was used to assess quality of care and satisfaction. Of 40,895 patients, 384 were adults (0.9%). Ages ranged from 18 to 73 years (median = 21 years, mean = 25 years) with 49% men. Most adults were seen between 3:00 PM and 10:59 PM; 84% were triaged as urgent or emergent. There were 140 new, and 230 chronic, patients. Fourteen were not classified because of lack of information. The mean age of new adults was 33 years, median 29 years; 53% were hospital employees. Forty percent of new adults had preexisting illnesses. Most common problems were cardiac/hypertension (27%), blunt trauma (22%), eye splash (13%), and lacerations (9%). Forty eight percent were transferred to an adult hospital, and 20% of transferred patients were admitted overnight. Chronic adult patients were younger (mean 21 years, P < 0.001); fever/infection (37%), minor trauma (17%), and gastro-intestinal problems (12%) predominated; 36% were admitted. The average time in the PED was 70 minutes for new and 202 minutes for chronic patients (P < 0.001). CONCLUSIONS: Chronic patients present with problems much like those of their younger counterparts, and the PED is prepared to manage them. New adult patients present with adult problems: chest pain, hypertension, blunt trauma, and eye foreign body, and the PED should be prepared to manage these.

Adolescent↗
PubMed1995

Credibility and outcome of cognitive-behavioural and psychodynamic-interpersonal psychotherapy.

Depressed psychotherapy clients (N = 117) rated the treatment principle credibility of cognitive-behavioural (CB) and psychodynamic-interpersonal (PI) before they were randomly assigned to receive either eight or 16 sessions of one of these treatments, and they rated their expectations of the treatment to which they were assigned immediately before (initial credibility) and immediately after their first session (emergent credibility). Results indicated that before they were assigned to a treatment, clients rated CB treatment principle credibility higher than PI treatment principle credibility. After assignment, however, clients rated initial credibility similar regardless of whether they were assigned to CB or PI therapy, and their ratings of emergent credibility increased to a similar degree from immediately before to immediately after the first session in both treatments. Clients' endorsement of CB and PI treatment principle credibility predicted improvement in PI therapy but not improvement in CB therapy. Initial and emergent credibility of clients' assigned treatment predicted improvement for clients who received eight sessions of therapy, but not for clients who received 16 sessions of therapy. The implications of these findings are discussed.

Adult↗
PubMed1995

In vivo expression of alternatively spliced forms of integrin-associated protein (CD47).

Integrin-associated protein (IAP) is a 50 kDa plasma membrane protein physically and functionally associated with beta 3 integrins in a variety of cells. IAP has an extracellular immunoglobulin domain, five transmembrane domains and a short intracytoplasmic tail. IAP is recognized by anti-CD47 antibodies and is expressed on cells, such as erythrocytes and lymphocytes, which do not express beta 3 integrins. To learn more about potential functions of IAP we examined its expression in vivo. Using the polymerase chain reaction, we detected 4 alternatively splice forms of IAP which differ from each other only at their intracytoplasmic carboxy termini. These alternatively spliced forms are generated by inclusion or exclusion of three short exons within 5 kb in the genome and are highly conserved between mouse and man. There is tissue specificity of expression of the alternatively spliced forms of IAP mRNA, with bone marrow-derived cells expressing predominantly one form and neural tissue another. Using polyclonal antibodies which recognize the alternatively spliced bone marrow (form 2) and neural (form 4) forms of IAP, we found that in accord with the mRNA, form 2 protein was expressed in all tissues primarily on bone marrow-derived cells and endothelia, while form 4 was highly expressed in the brain and peripheral nervous system. The evolutionary conservation of IAP isoforms and their tissue-specific expression suggest an important role for these intracytoplasmic domains in IAP function.

Alternative Splicing↗
PubMed1994

Effects of treatment duration and severity of depression on the effectiveness of cognitive-behavioral and psychodynamic-interpersonal psychotherapy.

A total of 117 depressed clients, stratified for severity, completed 8 or 16 sessions of manualized treatment, either cognitive-behavioral psychotherapy (CB) or psychodynamic-interpersonal psychotherapy (PI). Each of 5 clinician-investigators treated clients in all 4 treatment conditions. On most measures, CB and PI were equally effective, irrespective of the severity of depression or the duration of treatment. However, there was evidence of some advantage to CB on the Beck Depression Inventory (Beck, Ward, Mendelson, Mock, & Erbaugh, 1961). There was no evidence that CB's effects were more rapid than those of PI, nor did the effects of each treatment method vary according to the severity of depression. There was no overall advantage to 16-session treatment over 8-session treatment. However, those presenting with relatively severe depression improved substantially more after 16 than after 8 sessions.

Adult↗
PubMed1994

Associations of sleep with everyday mood, minor symptoms and social interaction experience.

Although there are strong popular beliefs about the value of a good night's sleep, there is very little documented evidence of day-to-day relations between sleep and well-being. In this study, covariations between sleep and both prior and subsequent daily states of well-being were studied in a healthy, employed sample. Thirty volunteers used pocket computers to complete a daily sleep diary and self-rating scales of mood, minor symptoms and social interaction experience. These were recorded every 2 hours for 14 days except during sleep periods. A pooled regression analysis showed small but significant relationships between many of the sleep and well-being measures. Sleep appeared to be more strongly related to subsequent well-being than prior well-being. An earlier onset of sleep was associated with better mood and social interaction experience the following day and was a better predictor than sleep duration. This result was interpreted to be consistent with the phase angle model of chronobiologic mood disorders. In general, the results suggest that the sleep disturbances found in affective disorders may not be pathological but instead represent the extremes of normal relationships between sleep and well-being.

Adaptation, Psychological↗
PubMed1993

Effect of moderate exercise on proliferative responses of peripheral blood mononuclear cells.

We studied the effects of 30 minutes of exercise on T lymphocyte counts and proliferative responses of peripheral blood mononuclear cells (PBMC) in 25 runners. Exercise resulted in a T lymphocytosis in the immediate post-exercise period in all subjects (p < 0.001), and reduced CD4+/CD8+ ratios in 22/25 subjects (p = 0.001). The change was due primarily to a 2.2-fold increase in CD8+ cells (p < 0.001). Exercise also reduced PBMC mitogenic responses to phytohemagglutinin (PHA) in 13/14 subjects (p = 0.049), and to pokeweed mitogen (PWM) in 11/14 subjects (p = 0.022), but not to concanavalin A. Postrun sera from 5 of 6 subjects inhibited PHA but not PWM responses of resting autologous PBMC with normal CD4+/CD8+ ratios (p < or = 0.05): indomethacin and monocyte depletion blocked the serum inhibition (p = 0.003, p = 0.0006, respectively). We conclude that post-exercise suppression of mitogenic responses to PHA is due to the release of a serum factor(s) capable of inducing prostaglandin synthesis by circulating monocytes, whereas exercise-induced suppression of PWM responses depends primarily on the reversal of CD4+/CD8+ ratios.

Adult↗
PubMed1993

RBRVS.

Explore the source record for details and available documents.

Health Care Reform↗
PubMed1992

Wind-induced accidents of road vehicles.

This paper describes the results of a project that collected and collated data for wind-induced vehicle accidents that occurred in the United Kingdom during the major storm of 25 January 1990. The results of this data analysis are used to determine which vehicles are most at risk during windy periods, the nature of the accidents that were caused, and the likely values of accident wind speeds. Also the adequacy of a computer program that can predict such wind speeds (the program BLOWOVER) is assessed as far as the available data allows.

Accidents, Traffic↗