PubMed1983
The platelet biosynthesis of total lipids, lipid fractions and fatty acids was determined by incorporation of [14C]acetate in normal and castrated rats of both sexes. Comparison was made between animals fed laboratory chow alone, and animals receiving, in addition, for 4 days by stomach tube a saturated (cream) or polyunsaturated (sunflower seed oil) fat. In male rats, the polyunsaturated fat increased slightly the total platelet lipid biosynthesis. The saturated fat drastically reduced it by 43% in comparison to the polyunsaturated fat-fed animals. Normal female rats did not exhibit a similar difference in the platelet lipid synthesis. However, the inhibitory effect of saturated fat on lipid synthesis could be observed in castrated females, although it was less pronounced (27% reduction) than in castrated or normal males (43%). Administration to castrated males of estradiol for 1 month almost completely inhibited the difference induced by the feeding of the different fats in the lipid platelet synthesis of male rats. This difference in the platelet lipid biosynthesis between male and female rats, normal and castrated, was observed mostly in the phospholipid (especially phosphatidylcholine), monoacylglycerol and triacylglycerol fractions and affected primarily the synthesis of the three main saturated fatty acids, 14:0, 16:0 and 18:0. Thus, it seems that, in rat, the short-term administration of a saturated fat induces drastic changes in the platelet lipid biosynthesis, but only in males. The protection observed in females appears to be essentially dependent upon estrogens.