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Biomedical subjects

S R Zhou

Publications and source records attributed to S R Zhou.

33 records · Page 2Linked to original sources
PubMed1990

An idiotype shared by monoclonal antibodies to different peptides of human myelin basic protein.

A mAb of the IgG1/kappa isotype was raised against human myelin basic protein (MBP) peptide acetyl 1-9. This mAb, termed F23, reacted with human MBP and human MBP peptides acetyl 1-9, 1-14, and 1-44, but not with MBP peptides 10-19, 80-89, or 45-89. According to the guidelines of the molecular recognition theory, a complementary peptide to human MBP peptide 1-9 was synthesized and used to raise murine mAb with anti-Id activity. Two mAb anti-Id, F25F7 and F25C8, both of the IgM/kappa isotype, were selected for further study. These anti-Id reacted with F23, the mAb for which they were selected, and also reacted with another mAb, which was of the IgG1/kappa isotype and was raised to human MBP peptide 80-89. There was no reaction with another control mAb of the IgG1/kappa isotype or murine myeloma IgG1. By immunoblotting techniques, it was demonstrated that the Id on each of the mAbs to MBP peptides was located on the kappa L chain but also could be recognized in nonreduced IgG. The cross-reactive anti-Id suppressed antibody secretion of Id-producing hybridoma cells in an Id-specific manner, and kinetic studies suggest an intracellular mechanism for the suppression. These cross-reactive Id among antibodies to different MBP peptides imply that the same V region genes of kappa L chains are involved in the selection of antibodies to an autoantigen, like MBP, and may play a role in the modulation of immune responses against MBP in certain inflammatory demyelinating diseases.

Amino Acid Sequence↗
PubMed1989

[Fracture-dislocation of pelvis in the adult female: clinical analysis of 105 cases].

Pelvic fractures with or without dislocation in 105 females, aged above 15 years, were studied. 47.6% of the fractures was through the antero-posterior ring. Among the complications, genital organ injury happened in 15 patients (14.5%): rupture of uterus in 5 (3 wombs conceived over 3 mon, 1 in period of nursing, 1 in normal state), rupture of ovary in 2, rupture of ovarian tube in 1 and laceration of vagina in 7. They were treated accordingly in urgent need. Follow-up of 1 to 5 years, averaging 5.3 years, showed various sequelae related to original pelvic trauma, of which accounts were given. It is worthy to emphasize that 45 of 75 patients (60%), who had become pregnant later, experienced dystocia due either to deformed pelvis or contracted vagina. 30 of the 45 patients had to undergo cesarean section. 15 other patients (14.3%) became barren following pelvic trauma, though they expected to be mother. Causes of the secondary sterility were discussed. Some patients experienced difficulty in sexual activity because of spoiled vagina, being involved by displaced bone fragment intrusion or by cicatricial contracture of itself. In short, in view of obstetrics, displaced pelvic fracture--dislocation should be reduced early following resuscitation of the patient, and be maintained steady by traction until union or by internal fixation if needed.

Adolescent↗
PubMedDate not supplied

The modulatory effect of anti-idiotypic antibody on hybridoma cells secreting antibody to human myelin basic protein peptide 80-89.

The idiotype (ID) of an antibody is postulated to be involved in immunoregulation by means of the immune network. This hypothesis was examined by studying the effect of a monoclonal antibody (MAb) anti-ID on hybridoma cells secreting an ID-bearing MAb, 845D3, to human myelin basic protein (MBP) peptide 80-89. The monoclonal anti-ID (IgM/kappa), reacted with heavy and light chains of 845D3, an IgG1/kappa MAb, but not with a control MAb of the same isotype specific for an MBP peptide differing by one amino acid residue. Fluorescence-activated cell sorting revealed IgG heavy and kappa light chains on the surface and in the cytoplasm of 90% or more of the 845D3 cells. The anti-ID did not react with the surface of the 845D3-secreting cells, and reacted with the cytoplasm of 14-18% of these cells. In spite of the absence of cell surface ID, the anti-ID significantly decreased antibody production by the ID-secreting cells. There was no effect of the anti-ID on control hybridoma cells secreting MAb to another MBP cells. There was no effect of the anti-ID on control hybridoma cells secreting MAb to another MBP peptide. Anti-ID exerted no cytotoxic effect on ID-bearing hybridoma cells and in fact caused a marginal increase in their proliferation compared to control MAb. These results indicate that an anti-ID may alter the antibody production of ID-secreting cells in a specific manner and without cytotoxicity. This may be one of the means for controlling an immune response against MBP generated by the cells of the immune system or in situ within the nervous system. The cellular mechanism(s) for this effect remains to be defined.

Animals↗