Biomedical subjects

S R Roberts

Publications and source records attributed to S R Roberts.

At least 19 recordsLinked to original sources

PubMed1991

Autotransfusion of unwashed mediastinal shed blood fails to decrease banked blood requirements in patients undergoing aortocoronary bypass surgery.

Infusion of unwashed mediastinal shed blood (MSB) is one technique advocated for decreasing use of donor blood in cardiac surgery patients. A commercially available system was prospectively evaluated in 96 consecutive patients. The control group was comprised of 78 consecutive patients. All underwent elective aortocoronary bypass surgery. Student's t-test, chi-square analysis, multivariate analysis, and Fisher's exact test were used where appropriate. There was no decrease in the amount of banked blood required or percentage of patients who received transfusions in the MSB autotransfusion group.

Blood Banks↗

PubMed1991

Analysis of the intracellular maturation of the herpes simplex virus type 1 glycoprotein gH in infected and transfected cells.

We have expressed the HSV-1 glycoprotein, gH, in transiently transfected COS-1 cells. The expressed protein was retained intracellularly, contained unprocessed carbohydrate, and was unrecognized by the monoclonal antibody, LP11. In addition, the protein was aggregated. These properties suggest that unlike other HSV glycoproteins, gH is misfolded in transfected cells. Pulse-chase studies of HSV-1-infected cells indicate that the kinetics of processing of gH are comparable to those of gB, gC, and gD. Rescue studies suggest that gH may interact with another protein during maturation in infected cells. However, we were unable to detect any stable interaction, although analysis of gH on neutral sucrose gradients shortly after synthesis indicated a possible transient association with a high molecular weight molecule or complex. The processing and cell surface expression of gH were also analyzed in HSV-1 virus mutants lacking gB, gC, or gD. Our results indicate that the maturation and cell surface transport of gH did not require the presence of these HSV-1 glycoproteins. In addition, three truncation mutants were constructed by linker insertion mutagenesis. Each of the three truncated proteins was synthesized, but the proteins were aggregated, contained only endo H-sensitive carbohydrate, and none were secreted.

Amino Acid Sequence↗

PubMed1990

Anxiety levels and cancer fear in patients admitted for elective operations.

Patients who are to have elective operations project varying degrees of anxiety, and many spontaneously express fear (without basis) that their operation involves a diagnosis of malignancy. To measure total, covert, and overt anxiety objectively, we gave the Institute for Personality and Ability Testing anxiety test to 125 consecutive patients admitted for elective general surgical procedures. A simple survey of cancer fear was also completed. Chi-square and Fisher's exact test were used to compare categoric data, and linear regression and analysis of variance were used where appropriate. Total anxiety scores were in the upper quartile compared to the general population. Scores indicating fear of cancer were elevated in 75% of patients who had no history of or reason to suspect malignancy. Covert anxiety scores correlated with cancer fear scores, and both significantly decreased as age increased (P less than .05). Also, as age increased, the cancer fear scores decreased (P less than .002). Obese patients had higher scores of cancer fear than all other patients (P less than .0001).

Adult↗

PubMed1990

Colonoscopic removal of a gallstone obstructing the sigmoid colon.

For the patient with a single gallstone obstructing the colon, this technique offers nonoperative relief of the obstruction, allowing definitive operative therapy to be accomplished at a later time on a more elective basis. The patient should be carefully observed during the interval for intervening recurrent gallstone ileus.

Aged↗

PubMed1988

Posttraumatic Pasteurella multocida meningitis.

The patient described was immunologically compromised by multisystem trauma. Pasteurella multocida was isolated from the respiratory tract and subsequently from the cerebrospinal fluid; direct spread apparently occurred by way of a basilar skull fracture. Sepsis was absent. He was successfully treated but subsequently had hydrocephalus, which has not previously been reported to occur after P multocida meningitis. Because of the opportunistic nature of this infrequent human commensal and the significant morbidity and mortality associated with it, we believe that isolation of Pasteurella multocida from the respiratory tract justifies treatment, especially in the compromised host.

Adult↗

PubMed1987

Surgical therapy of femoral artery pseudoaneurysm after angiography.

Seventy patients requiring surgical treatment for femoral artery pseudoaneurysm after angiographic procedures have been presented. The 10 year study period encompassed 38,822 catheter procedures (31,951 cardiology procedures and 6,871 radiology procedures) performed through the femoral artery. Presenting complaints included pain (51 percent) and neuropathy (19 percent). Additional morbidity has been discussed. The present study differs from previous ones in that many of the patients had femoral artery pseudoaneurysm formation in the common femoral artery without a history of smoking, diabetes, or hypertension. There was no demonstrable association with anticoagulation or antiplatelet therapy. The incidence of femoral artery pseudoaneurysm did increase with the use of a catheter sheath from 0.06 to 0.3 percent (p less than 0.005). This has not been reported previously. Direct arterial repair was utilized in 63 patients, whereas arterial reconstruction was required in 7 patients (saphenous vein graft in 4 and prosthetic graft in 3). There was one wound complication but no mortality associated with the surgical repair. Acceptable reasons for repair of femoral artery pseudoaneurysms are persisting or enlarging femoral artery pseudoaneurysms, associated pain or neuropathy, and rupture of femoral artery pseudoaneurysms.

Adult↗

PubMed1978