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Biomedical subjects

S R Jones

Publications and source records attributed to S R Jones.

At least 163 records · Page 9Linked to original sources
PubMed1978

An assay procedure for inositol pentaphosphate in avian erythrocytes.

A method to determine the concentration of inositol pentaphosphate (IP5) in chicken erythrocytes is described. Lysates of washed erythrocytes were treated with trichloroacetic acid to remove protein and were chromatographed on Dowex-1 chloride columns. After eluting the other organic phosphates with .3 N HCl, IP5 was eluted with .5 N HCl and quantitated by phosphorus assay. The mean for 12 chickens was 15.4 +/- 2.71 micromoles/g hemoglobin. The method should be useful for the assay of large numbers of samples.

Animals↗
PubMed1978

Changes in erythrocyte metabolism following acute blood loss in chickens.

The effect of acute blood loss on erythrocyte metabolism has been studied in a number of mammals. This study was designated to provide comparative data in chickens. Twenty to 30 ml of blood were removed from 7 chickens for 3 successive days. Packed red cell volumes were restored at the rate of 1.46% per day, and a maximum reticulocyte count (26.7%) occurred on the third day after the initial phlebotomy. Fourteen of the 16 erythrocyte enzymes measured became elevated significantly. Enzymatic activity was increased when the greatest numbers of young red cells were in the circulation and declined as the erythrocytes aged. Initial enzymatic activity was not related to the subsequent magnitude of increased activity. Inositol pentaphosphate, adenosine 5'-triphosphate, and reduced glutathione, but not 2,3-diphosphoglycerate, increased significantly in the postphlebotomy period. The markedly rapid and significant rise of inositol pentaphosphate suggested that it was controlled. That could be the way hemoglobin function is controlled during changing physiologic requirements.

Age Factors↗
PubMed1978

Pharyngeal colonization by gram-negative bacilli in aspiration-prone persons.

We compared the prevalence of Gram-negative bacilli in the pharyngeal flora of two groups of patients with a known predilection for Gram-negative bacillary pneumonia (chronic alcoholics and diabetics), two other groups of aspiration-prone persons with no known predilection for Gram-negative bacillary pneumonia (epileptics and narcotic addicts), and normal control subjects. Quantitative cultures of saline gargles showed pharyngeal Gram-negative bacilli to be significantly (P less than .05) more prevalent among alcoholics (35%) and diabetics (36%) but not epileptics (17%) or addicts (20%) than controls (18%). Counts of greater than or equal to 100 Gram-negative bacilli per milliliter were also significantly more common in alcoholics (14%) and diabetics (24%) than controls (5%, P less than .05). Enterobacter sp, Klebsiella pneumoniae, and Escherichia coli were the most common Gram-negative bacilli isolated. Increased colonization by Gram-negative bacilli might be a factor contributing to the propensity of alcoholics and diabetics for Gram-negative pneumonia.

Alcoholism↗
PubMed1977

Significance of antibody-coated bacteria in urinary sediment in experimental pyelonephritis.

The sequential appearance of antibody-coated bacteria in urinary sediment was followed in experimental pyelonephritis produced with Escherichia coli O6:K13:H1. It was possible to determine against which antigen of the organism this antibody was directed. Antibody-coated bacteria appeared by day 11 of infection, a time which coincided with the synthesis of local (intrarenal) antibody. Bacteria coated with antibody more than three days after serum antibody but four days before urinary antibody appeared. Antibody eluted from coated bacteria was directed against the O-antigen of the infecting organism but not the K-antigen. Newly synthesized intrarenal antibody and urinary antibody were directed only against O-antigen, but not against K-antigen; this finding would explain why the antibody that coated the bacteria was antibody to lipopolysaccharide. Thus, a positive test for antibody-coated bacteria indicates that a local immune response to O-antigen has occurred. It is postulated that this immune response could relate to the clinical symptomatology of acute symptomatic pyelonephritis since most patients presenting with acute pyelonephritis have bacteria coated with antibody.

Animals↗
PubMed1977

Haemophilus parainfluenzae infective endocarditis.

Seven young to middle-aged patients with Haemophilus parainfluenzae endocarditis are reported. Three patients had underlying heart disease and three patients had recent events predisposing for endocarditis. The clinical presentation was subacute or acute and new pathologic murmurs were uncommon. Diagnosis was prolonged because of difficulties in isolating the organism. Routine subculturing of blood cultures to chocolate agar with incubation in CO2 is recommended. A prominent complication, occurring in six patients, was major arterial occlusion secondary to emboli. Antibiotic control of infection was difficult and best achieved by the concomitant administration of ampicillin and gentamicin. Killing curves proved useful in assessing antibiotic efficacy. There were two medical failures and one death in the series. It appears H. parainfluenzae endocarditis is characterized by distinctive clinical features, difficult in vitro isolation of the organism, and the necessity for combination antibiotic therapy.

Adult↗
PubMed1977

An educational program for the rational use of antimicrobial agents.

A rational approach to the proper use of antibiotics must include knowledge of microbial sensitivities to various agents, proper alternative choices, pharmacologic considerations, and the cost to patient and hospital of drugs prescribed. A logical program for analysis of use of antibiotics and proper administration is presented. Since the largest misuse of antibiotics is for no infection or for prophylaxis before surgery, situations in which prophylactic use of antibiotics is of value have been reviewed. Such an educational program designed to improve the physician's use of antimicrobial agents must be a continuing effort in improving patient care.

Anti-Bacterial Agents↗
PubMed1977

Comparison of pharmacological and antimicrobial properties of cefadroxil and cephalexin.

Pharmacological and antimicrobial properties of cefadroxil, a new cephalosporin antibiotic, were compared with cephalexin. Absorption and excretion were studied in 20 healthy men. Peak concentrations of the drugs in serum were similar after ingestion of single 500-mg tablets. The concentration of cefadroxil in serum was more sustained than that of cephalexin. Levels of cefadroxil in serum after a dose of 1,000 mg were approximately twice those after a 500-mg dose through 6 h. Each drug administered in a dose of 500 mg every 6 h for 24 h resulted in concentrations in serum that were similar to a single dose without accumulation. Ninety-three percent of the cefadroxil and 85% of the cephalexin were excreted in urine after ingestions of single 500-mg tablets. The urine concentration of cefadroxil was more sustained than cephalexin. Minimal inhibitory and minimal bactericidal concentrations for clinical isolates were comparable with each drug. Cefadroxil compares favorably with cephalexin in this study. Sustained levels of cefadroxil in serum and urine suggest that this drug may be given at less frequent intervals than cephalexin.

Adult↗
PubMed1977

Further evaluation of the test for detection of antibody-coated bacteria in urine sediment.

To assess the reproducibility of the test for detection of antibody-coated bacteria in urine sediment as it might be used in a diagnostic laboratory for classification of urinary tract infections, multiple urine specimens from 83 patients were tested. The results were reproducible and consistent, or if inconsistent potentially explainable, in all but four patients. The explanation for inconsistencies include the immune response to the infecting bacteria, nonspecificity of the antibody coating the bacteria, antibody in prostatic secretions, and antibody-coated bacteria contaminating the urine specimens.

Antibodies, Bacterial↗
PubMed1977

The absolute granulocyte count in ascites fluid. An aid to the diagnosis of spontaneous bacterial peritonitis.

The absolute granulocyte count of peritoneal fluid was determined for 98 consecutive patients with ascites in order to assess its value as an early indication of bacterial infection. The absolute granulocyte count was found to be more sensitive and specific than either the total leukocyte count or differential count alone. When the absolute granulocyte count in ascites fluid is greater than 250 per cu mm, then it is likely that infection is present.

Ascitic Fluid↗
PubMed1976

Pseudocholelithiasis in an elderly man with calcified hydatid cysts.

A 69 year old man with intrabiliary rupture of a calcified echinococcal cyst mimicking acute cholelithiasis is discussed. This case is of interest because the correct diagnosis was not recognized preoperatively despite the fact that certain aspects of the illness were classic features of this complication of hydatid disease. Although this is a common complication of hydatid disease, which is well recognized in other countries, only seven cases have been reported in the American literature. Treatment of our patient included successful use of a Roux-en-Y drainage procedure which, to the best of our knowledge, has not previously been employed in treating this disease.

Aged↗
PubMed1976

Amino acid sequence of penicillopepsin. IV. Myxobacter AL-1 protease II and Staphylococcus aureus protease fragments and homology with pig pepsin and chymosin.

The digest of penicillopepsin (EC 3.4.23.7) with protease II from Myxobacter AL-1 gave five fragments which were separated on a Biogel P-100 column in 70% formic acid. The fragments were from 16 to 125 amino acids long. Two fragments were also isolated from a digest with a protease from Staphylococcus aureus. The analysis of these fragments by automatic sequencer gave a number of overlaps of the chymotryptic and thermolytic peptides. The available amino acid sequence data for penicillopepsin described in this paper and the accompanying papers (Kurosky, A. and Hofmann, T.:Can. J. Biochem. 54, 872 (1976);Rao, L and Hofmann, T.:Can. J. Biochem.54,885 (1976); Harris, C.I.,Rao, L., Shutsa, P., Kurosky, A. and Hofmann, T.: Can. J. Biochem. 54,895 (1976) have been combined and yield 15 fragments which range in lengths from 3 to 112 amino acid residues. These unique fragments account for virtually all the amino acids of the fungal protease. Four of the fragments with a total of 194 residues (about 60% of the molecule) have been aligned with corresponding sections of pig pepsin (EC 3.4.23.1) and with part of the N-terminal sequence available for calf chymosin (EC 3.4.23.4). In the alignments about 37% of the residues in the fungal enzyme are identical with at least one of the mammalian enzymes. An additional 20% are chemically similar. These results, together with previously reported active site directed modifications, show conclusively that penicillopepsin is an evolutionary homologue of the mammalian acid proteases.

Amino Acid Sequence↗
PubMed1976

Case reports: pleural melioidosis.

We report herein a patient with pulmonary melioidosis, whose initial chest roentgenogram revealed only a pleural mass. The patient had not been in a zone endemic for melioidosis during the previous 15 months. We stress the importance of including melioidosis in the differential diagnosis of pleural lesions, and of considering this diagnosis in any patient who has been in an endemic zone at any time in the past.

Adult↗
PubMed1975

Ballistic studies and lethal potential of tear gas pen guns firing fixed metallic ammunition.

Illegal older model and foreign-made tear gas pen guns as well as illicitly manufactured pen guns were used to fire revolver or pistol cartridges of calibers .22, .25, .32, .38, .44 Special, and .44 magnum, as well as rifle cartridges of caliber .270. Velocity, range of accuracy, impact kinetic energy, and gelatin block penetrability of bullets fired from the various cartridges are presented. The characteristics of recovered bullets, cartridge casings, and firing pin impressions are described, and the hazards of these weapons to users and potential victims are discussed.

Criminology↗