Studies on monoamine oxidase of rat heart mitochondria. I. Purification and properties of the enzyme.
Explore the source record for details and available documents.
Animals↗
Biomedical subjects
S R Guha
Publications and source records attributed to S R Guha.
Inhibition of purified rat liver mitochondrial monamine oxidase by trans-2-phenylcyclopropylamine.
Explore the source record for details and available documents.
Aldehydes↗
Monamine oxidation in different organs of rat.
Monoamine axodation has been studied in different organs of adult rats. Activity of monoamine dehydrogenase (MADH) has been measured both aerobically and anaerobically. Brain is the organ where maximum activity of MADH has been observed while in ovary the minimal enzyme activity has been noticed. In the absence of air, the activity proceeded at faster rate whereas MAO activity cannot take place in absence of oxygen. Oxygen can not be replaced by other electron acceptors like NTC. However, in some organs like pancreas, the formazan production was almost negligible. Aerobically moderate MADH activity was observed in case of ileum and testis. On the other hand, spleen, brain, testis and lung homogenates showed moderate amount of tetrazolium salts reduction in absence of air. This indicates the differential nature of the MADH activities in aerobic and anaerobic condition. High activity of monoamine oxidase (MAO) has been observed in liver. Brain and pancreas were also found good organs for MAO activity, but liver homogenate failed to reduce tetrazolium salt. Only dialysed liver homogenate in the presence of tryptamine, demonstrated moderate activity of MADH. Relative activity of both the enzymes has been studied. The organwise distribution pattern of MAO and MADH appeared quite different.
Aerobiosis↗
A dehydrogenase system to demonstrate oxidation of amphetamine and related compounds by rat brain homogenate.
Amphetamine, ephedrine, mescaline and chlorpromazine act as substrate for dehydrogenase system of rat brain homogenate. This system appears quite different from the usual deaminating process of these compounds, which required NADP and oxygen. The present system proceeds more rapidly in anaerobic condition.
Amphetamine↗
Sub-cellular distribution of monoamine oxidase and monoamine dehydrogenase.
MAO and MADH activities in different sub-cellular fractions of rat brain have been studied. The sub-cellular distribution pattern of MAO and MADH was found to be same, mostly concentrated in the mitochondrial fraction, although activities were also present in microsome and nucleus.
Animals↗