Biomedical subjects
Publications and source records attributed to S Power.
C reactive protein (CRP) concentrations are used routinely in the investigation and monitoring of neonatal sepsis. In this study, CRP responses were examined in the first week after birth. Among infants showing a clinically relevant CRP response, CRP concentrations > 60 mg/l were more likely with increasing gestational age.
OBJECTIVE: To evaluate the effects of laceback ligatures on the anteroposterior and vertical position of lower incisors and the mesial position of the lower first molars. DESIGN: Randomized controlled trial. SETTINGS: Patients under treatment in the Department of Orthodontics, Royal Bournemouth Hospital, Dorset, during a 6 month period from November 1999 to March 2000. SUBJECTS: Sixty-two adolescents (mean 13.7 years, range 11.2-16.8 years) with similar malocclusions, requiring extraction of all first premolars, were randomly assigned to experimental (laceback: 30; 12 male, 18 female) and control (non-laceback: 32; 14 male, 18 female) groups. INTERVENTIONS: Treatment using upper and lower fixed appliances following extraction of four premolars. One group had lacebacks placed, whilst the control group had no lacebacks. MAIN OUTCOME MEASURES: The participants were examined clinically and radiographically, and lateral cephalograms with radio-opaque tooth markers and lower study casts records were taken when lower fixed appliances were placed (T1) and following sufficient leveling with a 0.018 inch stainless steel round wire (T2). Linear measurements were recorded following digitization of the lateral cephalograms and using a vernier caliper on the study casts. A Student t-test was used to examine differences between the two groups following assessment for normality. RESULTS: In both groups the lower incisors retroclined during T1-T2; (Mean+/-SD: Experimental -0.53+/-1.9 mm, Control -0.44+/-1.29 mm). There was no statistical significance between the two groups (p = 0.84). The lower incisors extruded in both groups; 0.47+/-0.98 mm in the experimental group and 0.44+/-0.87 mm in the control group. There was no statistical difference between the groups (p = 0.9). The lower first molars showed 0.83 mm greater mesial movement in the experimental group, which was statistically significant (p < 0.05). Labial segment crowding decreased in both groups (experimental -3+/-1.6 mm, control -2.67+/-2.28 mm), the difference between the groups being non-significant (p = 0.51). Arch length decreased in both groups (experimental -2.08+/-2.82 mm , control -2.9+/-3.06 mm), but the difference between them was not significant (p = 0.28) CONCLUSIONS: In first premolar extraction cases, the lower labial segment does not procline during the leveling stage with the pre-adjusted edgewise appliance and the use of laceback ligatures conveys no difference in the anteroposterior or vertical position of the lower labial segment. Furthermore, the use of laceback ligatures creates a statistically and clinically significant increase in the loss of posterior anchorage.
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OBJECTIVE: To compare the clinical failure rate of pre-coated brackets and brackets bonded using Transbond XT light cure system OUTCOME MEASURES: (i) The clinical time required for bond up of upper and lower arches of both systems, (ii) bond failure rate for the first 6 months. DESIGN: Single centre randomized controlled clinical study. Thirty-three patients were bonded using a split mouth technique: randomly allocating the pre-coated brackets to upper left and lower right quadrants, and non-pre-coated brackets to the other quadrants. SETTING: Hospital Orthodontic Department, Basingstoke, Hampshire, UK. SUBJECTS: Orthodontic patients requiring fixed appliances. MAIN OUTCOME MEASURES: The site and time to bond failure was recorded for each bracket that failed over the first 6 months. The time required to bond upper and lower arches was measured using a stopwatch for each patient. RESULTS: t-Test for the difference of mean time needed to apply both groups of brackets, no significant difference (P > 0.2) was found. A chi-squared test for the difference in bracket failure between pre-coated (8.06%) and non-pre-coated (7.37%) showed no significant difference in bracket failure (P > 0.2). CONCLUSIONS: The clinical failure rate of pre-coated brackets is not significantly lower than conventional non-pre-coated brackets.
The crystal structure of an alkaline Bacillus cellulase catalytic core, from glucoside hydrolase family 5, reveals a novel combination of the catalytic machinery of two classic textbook enzymes. The enzyme has the expected two glutamate residues in close proximity to one another in the active-site that are typical of retaining cellulases. However, the proton donor, glutamate 139 is also unexpectedly a member of a serine-histidine-glutamate catalytic triad, forming a novel combination of catalytic machineries. Structure and sequence analysis of glucoside hydrolase family 5 reveal that the triad is highly conserved, but with variations at the equivalent of the serine position. We speculate that the purpose of this novel catalytic triad is to control the protonation of the acid/base glutamate, facilitating the first step of the catalytic reaction, protonation of the substrate, by the proton donor glutamate. If correct, this will be a novel use for a catalytic triad.
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Withholding gonadotrophin administration (coasting) may prevent severe ovarian hyperstimulation syndrome (OHSS). To ascertain the effectiveness of this protection a cohort of 252 consecutive in-vitro fertilization (IVF) or IVF with intracytoplasmic sperm injection (ICSI) cycles was studied. Twenty women with exaggerated response were treated with coasting. Despite coasting, four patients developed severe OHSS requiring hospitalization. Two of these four patients also required paracentesis. Multiple regression analysis in coasting cycles revealed severe OHSS was related to youth, number of oocytes retrieved and serum oestradiol prior to human chorionic gonadotrophin (HCG). Risk of developing severe OHSS was multifactorial and not necessarily prevented by withholding gonadotrophin.
Hepatocyte nuclear factor 4 (HNF-4) is an essential positive regulator of a large number of liver-specific genes. We report here the isolation of three HNF-4 isoforms from a human liver cDNA library. hHNF-4A and hHNF-4B, differing by the insertion of 10 amino acids in the C-terminal region, have been previously identified in mouse, rat and human liver. The novel isoform, hHNF-4C, is identical to hHNF-4A and B in the regions encompassing the DNA-binding and dimerization domains, but contains a different C-terminal domain. Similar to the other isoforms, hHNF-4C is produced in a limited number of tissues and represents 2.6-13% of the total hHNF-4 mRNA population, depending on the cell type. The chromosomal origin of all three isoforms has been localized to human chromosome 20. hHNF-4C can form heterodimers with hHNF-4A and B in vitro, and exhibits similar transactivation potential as hHNF-4A or B in transient transfection assays, suggesting that the divergent C-terminal region is not part of the transactivation domain.
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This paper provides new theoretical premises for understanding issues in 'bio-electromagnetics'. It firstly explains how converging magnetic fields, e.g. dipoles, can exert pressure on electrolyte distribution in an aqueous medium, and then it expands on this point to explain how this magnetic pressure is, in one extreme, a potential cancer causing force in the environment, and is, in another sense, a powerful new technique in cancer treatment. Included in this examination is a short analysis of the importance of this force in the morphogenesis of the fetus, and in the control of chromatic expression after the fact.
The homeoproteins HNF1 (LFB1/HNF1-A) and vHNF1 (LFB3/HNF1 beta) interact with an essential control element of a group of liver-specific genes. During development, these putative target genes are initially expressed in the visceral endoderm of the yolk sac and subsequently in fetal liver. To assess the possible involvement of HNF1 and/or vHNF1 as transcriptional regulators in the early steps of visceral endoderm differentiation, we have analyzed the expression pattern of both factors both in vitro during differentiation of murine F9 embryonal carcinoma cells and in vivo during early postimplantation mouse development. We show here that differentiation of F9 cells into either visceral or parietal endoderm is accompanied by a sharp induction in vHNF1 mRNA and protein. By contrast, only low levels of aberrantly sized HNF1 transcripts, but not DNA-binding protein, are found in F9 cells and its differentiated derivatives. At 6-7.5 days of gestation, high levels of vHNF1 mRNA are present in the visceral extraembryonic endoderm, which co-localize with transcripts of the transthyretin gene. HNF1 transcripts are first detected in the yolk sac roughly two embryonic days later, after the developmental onset of transcription of target genes. As development proceeds, discrepancies are observed between the level of transcripts of both vHNF1 and HNF1 and their respective nuclear binding proteins, notably in the yolk sac and embryonic kidney. In addition, we show that two alternative spliced isoforms of vHNF1 mRNA, vHNF-A and vHNF1-B, are expressed in both embryonic and adult tissues. Taken together, these data suggest that vHNF1 participates as a regulatory factor in the initial transcriptional activation of the target genes in the visceral endoderm of the yolk sac, whereas the later appearance of HNF1 could be required for maintenance of their expression. Our results also provide evidence of a posttranscriptional level of control of vHNF1 and HNF1 gene expression during development, in addition to the spatial restriction in transcription.
The Lactobacillus bulgaricus beta-galactosidase gene was cloned on a ca. 7-kilobase-pair HindIII fragment in the vector pKK223-3 and expressed in Escherichia coli by using its own promoter. The nucleotide sequence of the gene and approximately 400 bases of 3'- and 5'-flanking sequences was determined. The amino acid sequence of the beta-galactosidase, deduced from the nucleotide sequence of the gene, yielded a monomeric molecular mass of ca. 114 kilodaltons, slightly smaller than the E. coli lacZ and Klebsiella pneumoniae lacZ enzymes but larger than the E. coli evolved (ebgA) beta-galactosidase. The cloned beta-galactosidase was found to be indistinguishable from the native enzyme by several criteria. From amino acid sequence alignments, the L. bulgaricus beta-galactosidase has a 30 to 34% similarity to the E. coli lacZ, E. coli ebgA, and K. pneumoniae lacZ enzymes. There are seven regions of high similarity common to all four of these beta-galactosidases. Also, the putative active-site residues (Glu-461 and Tyr-503 in the E. coli lacZ beta-galactosidase) are conserved in the L. bulgaricus enzyme as well as in the other two beta-galactosidases mentioned above. The conservation of active-site amino acids and the large regions of similarity suggest that all four of these beta-galactosidases evolved from a common ancestral gene. However, these enzymes are quite different from the thermophilic beta-galactosidase encoded by the Bacillus stearothermophilus bgaB gene.
The three-dimensional structure of the subtilisin from Bacillus amyloliquefaciens (BAS) has been refined to 1.8 A using the amino acid sequence deduced from the DNA coding sequence. The structure is essentially the same as the previously reported structures of subtilisin BPN' (Wright, C.S., Alden, R.A., and Kraut, J. (1969) Nature 221, 235-242) and Novo (Drenth, J., Hol, W. G. J., Jansonius, J. N., and Koekoek, R. (1972) Eur. J. Biochem. 26, 177-181) determined in different crystal forms, at 2.5 and 2.8 A resolution, respectively. The largest differences in the three crystallographic models are seen in regions where the amino acid sequence used in the fit to the electron density maps of BPN' and Novo differs from the gene sequence of BAS (Wells, J. A., Ferrari, E., Henner, D. J., Estell, D. A., and Chen, E. Y. (1983) Nucleic Acids Res. 11, 7911-7925). The refined BAS model shows new features of cation binding, hydrogen bonding, and internal solvent structure. The refined BAS model has served as a basis for the analysis of stereochemical factors involved in the peroxide inactivation of the enzyme. Methionine 222, which is adjacent to the catalytic Ser221, is quantitatively oxidized to the sulfoxide by hydrogen peroxide as had been previously shown for the related Bacillus licheniformis enzyme (Stauffer, C. E., and Etson, D. (1969) J. Biol. Chem. 244, 5333-5338). In addition to this site of modification, we observe partial to full oxidation of two of the four remaining methionines. The oxidation of the methionines does not correlate well with their solvent accessibility calculated from the x-ray structure coordinates; in addition, only one of the two possible stereoisomers of methionine sulfoxide is formed. We also detect hydrogen peroxide-induced modification of the hydroxyl groups of two tyrosines. Modeling suggests that most of the observed effect of oxidation on the enzyme's catalytic efficiency can be attributed to unfavorable interactions at the oxyanion binding site between the sulfoxide group at 222 and the carbonyl oxygen of the scissile peptide bond of the bound substrate.
This paper describes the technique of sequential endoscopy and biopsy of the intact rat stomach. It is recommended that this procedure be performed on fasted animals with access to water only, when a mortality of only 4% can be achieved.
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