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Biomedical subjects

S Poppema

Publications and source records attributed to S Poppema.

At least 145 records · Page 8Linked to original sources
PubMed1986

Diagnostic application of a monoclonal antibody against small cell lung cancer.

A monoclonal antibody (MOC-1) directed against an antigen present in small cell lung cancer (SCLC) was used for diagnostic purposes. After screening of biopsy specimens of lung tumors, MOC-1 was found to react with SCLC (n = 10) and adenocarcinoma of the lung (4 of 9 cases). Except for a few cells in a poorly differentiated tumor, the reaction with squamous cell cancer was negative (n = 6). Staining with MOC-1 by an immunoperoxidase technique on imprints of biopsy specimens procured by rigid bronchoscopy was found to be a reliable and rapid method for diagnosing SCLC (16 of 17 positive). All cytologically proven bone marrow and pleural metastases of SCLC were found by staining on a cytospin preparation with MOC-1. Moreover, in three cytologically negative cases, MOC-1-positive cells were detected.

Antibodies, Monoclonal↗
PubMed1986

Evidence for a migratory capability of rat Kupffer cells to portal tracts and hepatic lymph nodes.

The present study concerns the migratory ability of Kupffer cells in the rat. Phagocytic cells were labeled with colloidal carbon or gold, these markers being administered intravenously either into a tail vein, which resulted in generalized reticuloendothelial uptake, or in low dose into the portal vein, which produced uptake by Kupffer cells alone. Cells containing marker were observed in the portal tracts and in hepatic lymph nodes from 1 to 3 days after injection into the portal vein. The direct movement of single marker particles to the portal tracts could be excluded. Since injection of marker into the portal vein labeled Kupffer cells exclusively, whereas blood cells, splenic and bone marrow macrophages remained unlabeled, the labeled cells in the portal tracts and hepatic lymph nodes appeared to be former Kupffer cells migrating which had migrated to these sites.

Animals↗
PubMed1986

Defined in situ enumeration of T6 and HLA-DR expressing epidermal Langerhans cells: morphologic and methodologic aspects.

An essential prerequisite for the in situ enumeration of epidermal Langerhans cells (LCs) is the unequivocal identification of the desired cell type. We have examined over 250 cryostat sections of normal human skin to analyze morphologic and methodologic problems underlying the quantification of epidermal LCs, defined by anti-T6 (OKT6) and anti-HLA-DR (OKIal) immunoperoxidase staining. Our findings show that OKT6 reactivity of dendritic processes in cross-sectioned epidermis yields microscopic images which are not easy to analyze objectively. The morphology that we find leads us to categorize dendritic cells into 3 arbitrary types of T6+ LC profiles. In addition we describe criteria for the assessment of OKT6 staining patterns relating to the dendritic state of epidermal LCs. Preliminary quantitative data on this issue are discussed in relation to: epidermal thickness; the thickness of skin tissue sections; and the discrepancy between the number of T6+ and HLA-DR+ LCs. We hope that the principles outlined in this report may serve to overcome potential methodologic problems with quantitation of T6+ epidermal LCs in skin sections.

Adult↗
PubMed1986

Nodular lymphocyte predominance type of Hodgkin's disease is a germinal center lymphoma.

Nodular lymphocyte predominance type of Hodgkin's disease can be distinguished from other subtypes of Hodgkin's disease on morphological and clinical grounds. Immunohistological studies on frozen tissue sections of seven cases of nodular lymphocyte predominance type of Hodgkin's disease (NLPHD) show differences in B cell, T cell, as well as dendritic cell population. NLPHD is confined to follicles which contain predominantly small lymphocytes, usually over 50% B cells and large numbers of B2+, anti-C3b+, anti-DRC+, and Ig- dendritic cells. The IgM+, IgD+, Leu8- B lymphocytes are of polyclonal origin. The T lymphocytes in these follicles are reactive with Leu7 in addition to T11, Leu1, T3, Leu3, and WT1. Leu7 is a monoclonal antibody reactive with natural killer cells, but also with a subpopulation of Leu3+ lymphocytes present in normal germinal centers. The population with this phenotype, not found in other types of Hodgkin's disease, appears to be greatly increased (up to 30%) in NLPHD. The so-called L&H type Sternberg-Reed (S-R) cells of NLPHD are transformed B cells, reactive with anti-B cell monoclonal antibodies which in some cases express detectable amounts of membrane and/or cytoplasmic immunoglobulin. Also, L&H type Sternberg-Reed cells in all cases stained for Ki-1 and Tac, and in three cases for LeuM1. Taken together, the findings indicate that NLPHD represents a proliferation of germinal center cells.

Antibodies, Monoclonal↗
PubMed1985

Morphologic, immunologic, enzymehistochemical and chromosomal analysis of a cell line derived from Hodgkin's disease. Evidence for a B-cell origin of Sternberg-Reed cells.

Cell lines derived from Hodgkin's disease may provide a clue to the nature of Sternberg-Reed cells. In the current study, the establishment of an Epstein-Barr-virus-negative lymphoblastoid cell line, derived from the pleural fluid of a patient with the nodular sclerosis type of Hodgkin's disease, is described. The morphologic and immunologic cell marker findings indicate that this cell line is derived from Sternberg-Reed cells. The immunologic findings and a chromosomal analysis are in agreement with a B-lymphocyte origin of these cultured cells. Extrapolation of the results to Hodgkin's disease in vivo would indicate that Hodgkin's disease, like most non-Hodgkin's lymphomas, is the result of B-cell proliferation.

Antibodies, Monoclonal↗
PubMed1985

Lymphocyte function-associated antigen 1 (LFA-1) is a marker of mature (immunocompetent) lymphoid cells. A survey of lymphoproliferative diseases in man.

Lymphocyte function-associated antigen 1 (LFA-1) is a membrane glycoprotein involved in a wide variety of functional activities mediated by human leukocytes. For example, expression of LFA-1 is required on immunoregulatory lymphocytes for functional activity. The appearance of LFA-1 in lymphocyte ontogeny was studied using leukemic cells as probes of distinct maturation stages. LFA-1 was expressed on the neoplastic cells of all mature T-cell neoplasias including T-prolymphocytic leukemia cells but not on immature T-cell blasts in acute lymphoblastic leukemia. The expanded T gamma-cell population in T gamma-lymphocytosis patients expressed very high amounts of LFA-1 compared to normal T cells. The malignant cells in B-chronic lymphocytic leukemia and common acute lymphoblastic leukemia were negative in all cases tested except one. In all B-prolymphocytic leukemia's and some of the B-Non Hodgkin's lymphoma patients the neoplastic cells expressed LFA-1. The plasma cells in two patients with plasmacytoma were found to lack LFA-1. It is concluded that LFA-1 is expressed on mature immunocompetent lymphocytes and their neoplastic counterparts.

Antigens, Surface↗
PubMed1985

Lymphadenopathy morphologically consistent with Hodgkin's disease associated with Epstein-Barr virus infection.

This case report describes a case of chronic Epstein-Barr virus infection resulting in lymphadenopathy morphologically indistinguishable from Hodgkin's disease. Morphologic, immunologic, and virologic studies could be performed on three consecutive lymph nodes. For the first time in the literature, Epstein-Barr Nuclear Antigen (EBNA) could be demonstrated in the nuclei of Sternberg-Reed cells.

Aged↗
PubMed1985

Neuroendocrine differentiation antigen on human lung carcinoma and Kulchitski cells.

In the normal lung, a subset of cells with a histological appearance consistent with that of Kulchitski cells are the only lung cells reacting with a monoclonal antibody (MOC-1) raised against a human small cell lung carcinoma-derived cell line. Outside the lung, a subset of normal endocrine cells (in the adrenal, thyroid, ovary, and pancreas) as well as neural cells (brain and peripheral Schwann cells) also express the antigen detected by MOC-1 (named MOC-1-related antigen). Some of these positively reacting cells are ectodermally derived, whereas others are of proven endodermal origin, indicating that the MOC-1-related antigen is not a cell lineage-specific antigen. Instead, the common expression of the antigen by cells with a neural, endocrine, or neuroendocrine function suggests that the antigen related to a neuroendocrine differentiation state of these cells. The presence of the MOC-1-related antigen on several non-lung tumors mostly paralleled its normal tissue distribution, indicating that the antigen is generally retained upon malignant transformation. In lung carcinoma, the antigen proves to be present on almost all small cell carcinomas tested. In addition, adenocarcinoma and mixed adenosquamous carcinoma could also express the antigen, whereas pure squamous cell carcinoma generally did not. This finding will be discussed in relation to a proposed "common stem cell" histogenesis of lung carcinoma.

APUD Cells↗
PubMed1985

Characterization of three new variant type cell lines derived from small cell carcinoma of the lung.

Three new, well growing cell lines (GLC-1, GLC-2, and GLC-3) have been established from small cell lung carcinoma (SCLC) and characterized. A subclone (GLC-1-M13) markedly different from its parent line GLC-1 was also isolated and characterized. Cytogenetic analysis of the cell lines revealed deletions in the short arm of chromosome 3 as a most consistent chromosomal aberration. The deleted region was not identical in all metaphases, 3p(21-23) being the shortest region of overlap. Despite their SCLC origin GLC-1, GLC-2, and GLC-3 do not show pronounced SCLC differentiation features. Neurosecretory granula were very rare (GLC-1) or completely absent (GLC-2 and GLC-3), whereas the SCLC-related enzyme and hormone markers L-3,4-dihydroxyphenylalanine decarboxylase, neuron-specific enolase, creatine kinase BB, and bombesin-like immunoreactivity were variably expressed. Although the subclone GLC-1-M13 was derived from the poorly differentiated GLC-1, it behaved according to the above criteria as a differentiated "classic" SCLC cell line. When assessed with specific monoclonal antibodies the different cell lines appeared to express different subsets of intermediate filament proteins, indicative for different stages and directions of differentiation: "undifferentiated" (GLC-1 and GLC-2); "neural tissue related" (GLC-2); "simple epithelium" related (GLC-1-M13); and a combination of simple and squamous epithelium related (GLC-3). We conclude that GLC-1, GLC-2, and GLC-3 represent dedifferentiated forms of SCLC, related to the recently described "variant" type of SCLC, whereas the clonal derivate GLC-1-M13 behaves like a differentiated "classic" SCLC cell line.

Carcinoma, Small Cell↗
PubMed1985

Lymphocyte compartments in human spleen. An immunohistologic study in normal spleens and uninvolved spleens in Hodgkin's disease.

A panel of monoclonal antibodies directed against T and B lymphocyte antigens was used to analyze the presence and localization of several lymphocyte subsets in 13 normal human spleens (3 of newborns) and 17 uninvolved spleens of patients with Hodgkin's disease. The distribution of cells in the white pulp corresponded with findings in other secondary lymphoid organs, except for the presence of a marginal zone, a unique compartment localized at the border of white and red pulp. The phenotype of the marginal zone cells indicates that it is likely that the marginal zone contains nonrecirculating as well as recirculating B cells, while T cells (of the T helper type) are also represented. Therefore, the notion that marginal zone cells are nonrecirculating IgM+, IgD- cells, appears to be an oversimplification. No clear differences were observed between spleens of patients with and without Hodgkin's disease.

Adolescent↗
PubMed1985

DNA measurements in thin sections of lymphomas.

The DNA content of the nuclei of lymphomas and a reactive lymph node was studied by light absorption measurements in Feulgen-stained thin (2 microns) sections of lymph node biopsies, using the TAS image analysis system. For each nucleus, the integrated light absorbance at 548 nm wavelength was multiplied by the square root of the nuclear area to obtain a parameter for DNA independent from the nuclear size. In a hyperplastic reactive lymph node with follicular hyperplasia, the distribution of DNA X square root area was different in centrocytes and centroblasts, being compatible with a diploid centrocyte fraction and a mainly tetraploid centroblast fraction, in which hypertetraploid (octoploid?) nuclei were present. Similar measurements in three follicular centroblastic-centrocytic lymphomas gave similar results, with the centroblasts mainly tetraploid. The DNA distribution was studied in sections of lymphomas of high and low grades of malignancy, classified visually on the number of mitoses seen per field. The distribution width, from the smoothed histogram as the DNA X square root area values beyond the maximum peak frequency, appeared to be larger in the high-grade malignant lymphomas, in accordance with their higher number of mitoses.

Biopsy↗
PubMed1984

Immunoperoxidase staining on frozen tissue sections as a first screening assay in the preparation of monoclonal antibodies directed against small cell carcinoma of the lung.

To prepare monoclonal antibodies against small cell carcinoma of the lung (SCCL), we have used an SCCL-derived cell line as immunogen. A first screening of hybridoma supernatants was performed on frozen tissue sections of a biopsy with histologically proven SCCL involvement. Screening on tissue sections is a valuable technique, especially for the isolation of monoclonal antibodies directed against tumor antigens. A drawback of this procedure is that it is laborious. To circumvent this, we have reduced the number of supernatants to be screened by increasing the number of seeded hybridomas per well. Although this resulted in the growth of 5-20 hybridomas per well, among which there was always at least one that secreted antibodies against 'common antigens', clones that secreted specific antibodies could still be revealed by testing supernatants which were preabsorbed with thrombocytes. This has resulted in the isolation of 7 monoclonal antibodies directed against SCCL associated antigens.

Antibodies, Monoclonal↗
PubMed1984

Prognostic factors and changing trends in the treatment of Stage I endometrial cancer: a clinical and histopathological study of 182 patients.

Changing trends in treatment of Stage I endometrial carcinoma are reflected in the modalities used in the University Hospital in Groningen. From 1969 to 1979, three treatment regimes have been used in 182 patients with Stage I disease: Group A (50 patients): preoperative uterine radium packing followed by surgery six weeks later; Group B (94 patients): preoperative low dose external irradiation (14 Gray) immediately followed by surgery and an additional 40 Gray in patients who had invasion of the myometrium beyond the inner one-third; Group C (38 patients): primary surgery with post-operative radiation therapy. No significant differences were found in the 5-year actuarial survivals of 92, 90 and 88% in Groups A, B and C respectively. Most recurrences were seen in Group B (16%) as compared to 10% in Group A and 8% in Group C. Poor prognostic factors were found to be myometrial infiltration beyond the inner one-third, dedifferentiation of the tumor (Grade 2 and 3), vascular invasion, age over 60 years, and uterine length over 8 cm. The advantages of primary surgery, allowing for accurate surgical-pathological examination and individualization of postoperative irradiation, are stressed.

Adenocarcinoma↗