The primary structures of three major species of human leukocyte interferon differ from the structure predicted from the DNA sequence of recombinants containing leukocyte interferon-coding regions. Compared to the recombinant interferon produced in bacteria, three of the purified natural proteins isolated from leukocytes lack the 10 COOH-terminal amino acids suggested by the DNA sequence.

Amino Acid Sequence↗

PubMed1981

Production of hybridomas secreting monoclonal antibodies to the human leukocyte interferons.

Thirteen monoclonal antibodies to human leukocyte interferon have been obtained. They exhibit different patterns of binding to purified leukocyte interferon species that are consistent with the structural multiplicity of the human leukocyte interferons. These antibodies will be useful as probes into the structure of the human leukocyte interferons, for their purification, and for rapid assay of leukocyte interferon.

Animals↗

PubMed1981

Cell cultures producing human interferon.

Most of 27 B-lymphoblast and 5 other cell lines derived from human leukocytes were found to produce human interferon either constitutively or after induction with Newcastle disease virus. Several produced relatively high levels of interferon after induction.

B-Lymphocytes↗

PubMed1981

Production of high levels of human leukocyte interferon from a continuous human myeloblast cell culture.

A myeloblast cell line has proved to be an excellent source of human leukocyte interferon. These cells, primed with interferon and induced with Sendai virus, produced optimal levels of human leukocyte interferon. The cells grew readily in spinner flasks and in medium containing horse serum. Interferon production over several months yielded an average titer of 2 X 10(5) reference units of interferon per 10(7) cells. The interferon produced by these cells appeared to be predominantly species of human leukocyte interferon. Since these cells seemed to consist of myeloblasts, it is clear that cells other than B-lymphocytes can produce leukocyte interferon.

Cell Line↗

PubMed1981

Convenient assay for interferons.

A convenient assay for interferons based on reduction of cytopathic effect was developed. The number of manipulations and the lengths of the various incubation steps were reduced to a minimum. The assay is simple to perform and can be completed within 16 h. Moreover, it can be used with various types of cells and a variety of viruses.

Animals↗

PubMed1981

Interferons. Part B. Introduction.

Explore the source record for details and available documents.

Amino Acid Sequence↗