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Biomedical subjects

S Perry

Publications and source records attributed to S Perry.

At least 109 records · Page 6Linked to original sources
PubMed1976

Oncogenesis in human acute leukemia.

By selecting, for discussion, some aspects of oncogenesis in human acute leukemia, we hope to focus attention on areas of current interest which promise to provide important advances in our knowledge and understanding of human neoplasia, and, in time, may lead us nearer to the goal of disease control and eventual eradication.

Acute Disease↗
PubMed1976

Lysosomal acid hydrolases in human lymphocyte subpopulations.

Acid phosphatase and beta-glucuronidase activities have been determined cytochemically in T, B and null lymphocytes as part of an effort to charaterize the human haemopoietic stem cell. A combination of weak acid phosphatase activity and strong beta glucuronidase staining, in the form of a single large granule, has been shown to be specific for T cells. On the basis of this approach alone, non-T cells could not be further subclassified. Further cytochemical evaluation is being explored.

Acid Phosphatase↗
PubMed1976

Lymphocyte purification from normal human peripheral blood by filtration through columns of cotton wool.

With a view to simplifying the separation of haemopoietic stem cell concentrates from human peripheral blood, in preparation for their clinical use, the production of lymphocyte concentrates by cotton wool filtration has been investigated. Using the method which is described in detail, highly purified suspensions may be prepared without the selective loss of thymus-independent lymphocytes. Optimal operating conditions are defined. Haemopoietic stem cell concentrates may thereby be harvested, and are shown to be capable of proliferation and differentiation on subsequent culture in vivo.

Cell Separation↗
PubMed1976

A reappraisal of cellular labeling with radiochromium salts.

The present studies demonstrate that, while erythrocytes preferentially bind hexavalent 51Cr, leukocytes bind the trivalent form with equal avidity. Red cells may be saturated with label in contrast to the binding of 51Cr to leukocytes, which may be described by first-order kinetics. Hexavalent label is rapidly reduced in leukocytes in contradistinction to the finding in red cells. The nonreutilization of label by erythrocytes and leukocytes is not due to inappropriate valency but to protein binding of the label. Unlike nonleukemic cells, leukemic leukocytes elute a large fraction of their label. These factors have been largely overlooked in many previous clinical studies using labeled cells, and they are of sufficient quantitative importance to compromise seriously the validity of numerous earlier reports.

Animals↗
PubMed1975

Hemopoietic stem cells in human peripheral blood.

A population of lymphocytes, separable from the great majority by virtue of their larger size and their failure to exhibit the rosetting characteristics of thymus-dependent lymphocytes and bursa-equivalent cells, possess true pluripotentiality. On culture in vivo they proliferate and differentiate into erythrocytic, granulocytic, and megakaryocytic progeny. This may be the first clear demonstration of the primitive progenitor blood cell in man.

Cell Nucleus↗
PubMed1975

Study of leukocyte kinetics in acute myelocytic leukemia utilizing chromium-51.

Leukocyte kinetic studies using chromium-51 were performed in four patients with acute myelocytic leukemia (AML). Intravascular leukocyte survival was prolonged in comparision with granulocyte survival in normal subjects. Significant splenic pooling occurred in three patients, none of whom had splenomegaly. In one patient studied, circulating leukemic cells were shown to return to the bone marrow. The prolongation of intravascular leukocyte survival in AML in relapse, as in chronic myelocytic leukemia, probably depends on several factors including the presence of immature leukemic cells and the recycling of these cells from the spleen and bone marrow.

Adult↗
PubMed1975

Glucocorticoid binding proteins in myeloblasts of acute myelogenous leukemia.

The myeloblasts of 16 patients with untreated acute myelogenous leukemia were examined for specific glucocorticoid binding proteins. In only 3 of the 16 patients were such high affinity receptors found. The presence of glucocorticoid binding protein activity was positively correlated with inhibition of macromolecular synthesis of myeloblasts in vitro whereas absence of binding protein activity was associated with lack of glucocorticoid inhibition of the cells. Since glucocorticoids appear efficacious in only a small fraction of cases of acute myelogenous leukemia, determination of glucocorticoid binding protein activity may provide assistance in planning chemotherapeutic regimens.

Bone Marrow↗