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Biomedical subjects

S Pedersen

Publications and source records attributed to S Pedersen.

At least 253 records · Page 14Linked to original sources
PubMed1981

Cloning, restriction endonuclease mapping and post-transcriptional regulation of rpsA, the structural gene for ribosomal protein S1.

Transducing lambda phages have been isolated that carry segments of the Escherichia coli chromosome in the aspC region, 20.5 min on the E. coli map. One of these phages, lambda aspC2, carries rpsA, the structural gene for the ribosomal protein S1. A three kilobase fragment from this phage, cloned into either the plasmid pACYC184 or the plasmid pBR322, was found to express S1. In cells carrying the rpsA gene on the high copy number plasmid pBR322 the rate of rpsA mRNA synthesis was increased 40-fold, whereas the rate of protein S1 synthesis was doubled, in comparison with these rates in an rpsA haploid.

Cloning, Molecular↗
PubMed1981

Rectal administration of theophylline in aqueous solution.

Theophylline has a narrow therapeutic index which complicates rapid effective administration. Therapeutic serum levels without toxic concentrations can usually be achieved only by a 20 min intravenous administration. In the present study 20 children were given a single dose of theophyllinne in aqueous solution rectally. Serum theophylline levels were determined, and they equalled those found after a 20 min intravenous infusion of theophylline. No adverse effects were observed. The variations in peak serum levels were the same as those reported after intravenous administration. Therefore rectal administration of an aqueous solution of theophylline may become a valuable alternative to the intravenous route. The procedure is simple, can be used at home by the parents even in cases of nausea and vomiting, and an accurate dosage calculated in mg/kg is possible. This treatment, however, cannot be recommended until further investigations have been carried out.

Asthma↗
PubMed1979

Post-translational modification of Escherichia coli ribosomal protein S6.

Escherichia coli has multiple forms of ribosomal protein S6, differing in number of glutamyl resideus at the C-terminal end. Three forms are revealed when crude cell extracts are fractionated by a two-dimensional gel electrophoresis technique. Pulse-chase experiments show that the shortest and most alkaline form of S6 is the first to appear. In about one doubling time this form reaches equilibrium with the two other forms of S6, implicating the existence of an enzyme, which adds glutamic acid residues to S6. We show that the relative levels of these three S6 forms are not affected by the growth rate of the culture.

Amino Acid Sequence↗
PubMed1979

Psychosocial support for adults with cystic fibrosis: a group approach.

This article describes the first time-limited psychosocial support group for adults with cystic fibrosis (CF). The chronology of the group is traced, focusing on issues of importance for health professionals who work with patients with CF. Some of the issues raised by patient members include doubts about the competence of nonpediatricians to adequately treat CF; resentment towards "normals" (those without CF), including health professionals; and the desire to control as many areas of their lives as possible. Covert (ie, unrecognized) issues, the role of staff, risks, and outcome are detailed as a guide for others who may wish to conduct such groups. The literature on groups for medical patients and a rationale for the beneficial effects on the group are given.

Adaptation, Psychological↗
PubMed1979

Intestinal lesions caused by incorrectly placed seat belts.

Employment of seat belts reduces the number of fatal accidents and alters the pattern of lesions. However, seat belts themselves can produce serious lesions, particularly if incorrectly placed. Three cases of traumatic intestinal perforation are mentioned. Intestinal perforation often produces few initial symptoms. The diagnosis is difficult and is frequently established late. If marks are present on the abdomen from the seat belt, the patient should be admitted for observation. The possible pathogenesis of the intestinal lesions are mentioned. The seat belt should be correctly placed in order to obtain optimal effect. Reasons for incorrect placing are mentioned. It is important that the seat belt is adjusted to the passenger employing it.

Accidents, Traffic↗
PubMed1978

Functional mRNA half lives in E. coli.

Analysis of the synthetic rate of individual protein species at various times after complete inhibition of transcription with either streptolygidin or rifampicin was carried out by two-dimensional polyacrylamide electrophoresis of total Escherichia coli cell extracts. The decay rate of the potential to synthesize different proteins was assumed to be equal to the functional decay rate of the corresponding mRNA. We conclude the following: (a) The tufA and tufB messengers have different half lives (3.0 and 2.4 min, respectively). (b) Different genes within the same transcriptional unit can have different half lies (S7, EGF and EFTuA--2.5, 3.8 and 3.0 min, respectively). (c) There is at least a twenty-fold variation in individual mRNA half lives in E. coli; ribosomal protein S1 mRNA was observed to have the shortest half life in the cell (40 sec), while the longest observed half life was approximately 20 min (all values at 30 degrees C). (d) Addition of rifampicin increases the absolute rate of RNA polymerase subunit alpha and beta synthesis two-fold. (e) The induction of the synthesis of alpha subunit of RNA polymerase takes place without a concomitant induction of ribosomal protein S4 and L17, which are reported to be on either side of alpha in the same transcriptional unit.

Aminoglycosides↗