Negative interference with the Du Pont aca method for measuring digoxin--a reply.
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Autoanalysis↗
Biomedical subjects
S P Crouse
Publications and source records attributed to S P Crouse.
Radioimmunoassay of human pancreatic amylase in serum and urine.
The purification of human pancreatic amylase (EC 3.2.1.1) and the development of a radioimmunoassay for this enzyme is described. The enzyme was purified from a normal pancreas obtained at autopsy and antibodies were produced in rabbits. The purified pancreatic amylase was radioiodinated and the radioimmunassay was developed. Analytic recoveries in serum and urine specimens were 99 and 96%, respectively, and day-to-day precision was less than 10%. The assay gave a 20% cross-reactivity with salivary amylase.
Amylases↗
Rapid enzymatic determination of amylase in serum and urine using a centrifugal analyzer.
A convenient, totally enzymatic procedure for amylase assay using a centrifugal analyzer is described. The reaction scheme is examined using both starch and maltotetroase as substrates. Data for the reaction kinetics, linearity and sensitivity are presented. The proposed method exhibits linear reaction kinetics between three and five minutes after the initiation of the reaction and is linear to 1200 U per liter of amylase. The determination is rapid, (five minutes analysis time), convenient, (only one reagent), and precise, (1.2% within-day C.V. and 4.6% day-to-day C.V.), at a level of 62 U per liter. The proposed method compares favorably with chromogenic procedures; (r = 0.979, n = 84).
Amylases↗