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Biomedical subjects

S Ottó

Publications and source records attributed to S Ottó.

At least 37 records · Page 2Linked to original sources
PubMed1989

[The significance of analysis and detection of Bence Jones protein in the urine in clinical laboratory diagnosis].

Bence Jones proteins (monoclonal free light-chains of immunoglobulins) are the earliest known biologic markers of malignant plasma cell dyscrasia, but Bence Jones proteinuria is also found in many types of B-cell related neoplasms. In some cases it may occur in Hodgkin disease. In some cases benign monoclonal gammopathy related to non-tumor conditions was found. The type of the monoclonal light-chain, the polymerisation and the isoelectric point of the molecules are considerable factors in the development of the disease. Urine specimens from 637 patients with (or suspected) lymphoproliferative diseases were investigated in a two year period using different immunochemical methods. Bence Jones proteinuria was found in 71 cases with the new method (isoelectric focusing combined with immunofixation) and 63 cases by the conventional ones. Bence Jones proteins can be detected by this new method at level below the sensitivity of conventional procedures. Bence Jones proteins in the urine may call the attention to the malignant tumor or the malignant transformation of the disease. The early detection of the monoclonal light-chain excretion may be important in the respect of clinical diagnosis, therapy and the follow-up the clinical status of the patient.

Antibodies, Monoclonal↗
PubMed1989

[Survey of the nutritional status of patients with locally advanced laryngo-pharyngeal tumors].

At the Department of Head and Neck Surgery of the Postgraduate Medical University the authors surveyed the nutrition state of 20 patients with stage III-IV locally advanced pharyngolaryngeal tumours. The control data were provided by 20 patients with benign lesions. It can be established that among the hematological and biochemical tests the studies of the N-balance, the serum transferrin as well as the antropometric measurements are objective markers of the protein-calory malnutrition of the cancer patients. The authors emphasize, however, that the immunological investigations need further purification.

Alcohol Drinking↗
PubMed1986

Gamma-chain heavy-chain disease with primary thyroid plasmacytoma.

A case of gamma-chain heavy-chain disease was associated with primary thyroid plasmacytoma. The patient has been free of complaints for two years since the removal of the tumorous thyroid. The heavy-chain fragment (molecular weight, 32,000 daltons) has disappeared from the serum and urine, while the previously low levels of normal immunoglobulins have increased. The immunoglobulin anomaly may be correlated with the chronic antigen stimulus.

Aged↗
PubMed1986

Correlation between the results of carcinoembryonal antigen (CEA) test and the clinical stage of colorectal carcinoma.

Correlation between results of CEA test and clinical stage of colorectal carcinoma is described. No correlation was found between the different stages and the actual CEA titre. Normalization of an increased preoperative serum CEA level indicated, however, nearly always the radical character of the intervention. Critically high (above 30 ng/per ml) CEA value observed in Dukes' stages C and D can be considered bad prognostic signs. Patients like these died within one year. Results of CEA tests are also useful complementary data contributing to the diagnosis of recurrence or distant metastases.

Adult↗
PubMed1984

Immunoselection screening methods for detection of the free heavy chains.

Immunoselection screening methods were developed for the detection of the free heavy chain determinants of biological fluids. The optimal sensitivity range of the electrosyneresis and electroimmunoassay type methods lies between 400 ng and 2 micrograms/ml and between 3 micrograms and 1 mg/ml for immunoglobulins. The sensitive, relatively rapid and cheap variants of the procedure are suitable for the detection of "heavy chain diseases" and of heavy chain fragments.

Heavy Chain Disease↗
PubMed1984

Subsequent monoclonal gammopathies.

21 patients with 'subsequent' monoclonal gammopathies which developed after non-tumour or tumour states were studied. The cases were selected from 221 patients producing M protein, partly based on retrospective analysis and partly on data of case history, autopsy, histology, and immunochemistry obtained during continuous observation. Possible ways of development of immunoglobulin-producing monoclonal cell clones are also discussed.

Adult↗
PubMed1984

Immunofixation electrophoresis: an immunochemical method for testing protein anomalies.

The practical problems of immunofixation electrophoresis are discussed and the advantages of the method are stressed on the basis of data in the literature and own experience mainly in tests of monoclonal gammopathies. Technical modifications are recommended by which the tests can be performed easily and quickly with small amounts of immune sera.

Blood Protein Electrophoresis↗
PubMed1983

Simple screening of Bence-Jones proteins from unconcentrated urine.

A rapid, sensitive and simple method is provided for detecting Bence-Jones proteins in unconcentrated urine by electrosyneresis. This is based on the fact that the free light-chains, similar to the intact immunoglobulin molecules, form complexes with albumin under the effect of glutaraldehyde. Consequently, the originally cathode-oriented Bence-Jones proteins will also migrate toward the anode against the corresponding specific immunoserum. At a 200 mg/ml excretion rate of the free light-chain (corresponding to 0.3 mg/24 h Bence-Jones protein in 1500 ml daily urine) the reaction is still readily assessed. The method presented here meets the requirements of a routine clinical laboratory.

Antigen-Antibody Reactions↗
PubMed1983

Monoclonal cryoimmunoglobulinemia of IgG4 subclass specificity.

Authors demonstrated the prevalence of an unusual cryoimmunoglobulin with IgG4 subclass specificity in the blood of a patient with malignant lymphoproliferative disease. They provide the case history, results of immunochemical studies, and cytological and ultrastructural findings of the bone marrow.

Bone Marrow↗
PubMed1982

Reversed immunofixation agar gel electrophoresis.

A method has been elaborated for investigation of biological fluids on the basis of the theory of the immunofixation electrophoresis. In spite of the usual methods of the immunofixation electrophoresis it is the antibody which moves by the electrophoresis and not the antigen. The antibody stains are fixed with cellulose acetate strip soaked with a suitably diluted antigen solution after electrophoresis. This method uses only a small quantity of the immune serum.

Antibodies↗
PubMed1982

Determination of immunoglobulin subclasses by electrosyneresis.

The author applied the method or electrosyneresis to distinguish the subclasses of monoclonal IgG and IgA immunoglobulins. In agarose gel with high electroendosmosis the antibody-active fraction of immunosera migrates toward the cathode while the immunoglobulin molecules bound to albumin by glutaraldehyde toward the anode. In the meeting zone of specific immunoserum and immunoglobulin--albumin complex a precipitation arch develops if immunochemical identity exists. This method is extremely sensitive, economic and rapid. It is very suitable for the distinction of the subclasses of monoclonal immunoglobulins.

Counterimmunoelectrophoresis↗
PubMed1982

Simple method for the screening of polymeric Bence Jones proteins.

The author tested the electropherograms of previously identified, concentrated urine samples in agarose gel. Before electrophoresis, the gel plate containing the samples was covered with a cellulose-acetate membrane soaked with 0.1 M 2-mercaptoethanol, and it was incubated for 10 min in a moist chamber, at room temperature. If the polymeric Bence Jones protein is present, the electropherogram of the sample changes. In the following, a quick and easy method of recognizing this rare structural anomaly is recommended.

Bence Jones Protein↗
PubMed1980

"Diclonal" gammopathies.

Eleven patients with "diclonal" gammopathies have been followed clinically and cytologically in relation to the changes in their serum protein composition. It was observed that the malignant transformation became faster after a second protein had appeared with the appearance of immature lymphoid elements and plasmoblasts. Malignant transformation may occur after long periods of stagnation, therefore a continuous observation of such cases is mandatory.

Aged↗
PubMed1979

Increased responsiveness of electric precipitation (electrosynerese) by means of a chemical method.

Electric precipitation is a method in serological diagnostics (determination of species-specificity). Since serum protein fractions (especially immunoglobulins) and extracts from blood stains migrate in agar-gel at pH 8.2--8.6 towards the cathode they cannot be used for the determination of the species specificity. By a chemical method using glutaraldehyde authors have produced an albumin-immunoglobulin complex, altering in this way the direction of electrophoretic mobility. Treatment of the blood samples with glutaraldehyde increases the sensitivity and realiability of the method.

Blood Protein Electrophoresis↗
PubMed1976

[Hypoxia and polytrauma].

The importance of polytrauma and hypoxia, resp., is discussed by the authors with regard to the mortality and the common effect of these is analysed. It was found that in the mortality of the patients suffered polytraumatism the first place (59,4%) is taken by such lesions, in which simultaneous lesion of the skull and the thorax occurs. The values of the blood gas and the acid-base balance of the patients who had suffered polytraumatism, were examined in the 12th--24th hours proceding the death. It was found that whereas--using the possibilities of the intensive therapy--the values of pH, paCO2 and BE could be kept in a part of the cases between the physiological limits--the paO2 value was under the normal value in all cases--moreover 2/3 of the values fell into the domain between 40--60 mmHg--signifying severe hypoxia. On the basis of the analysis it may be presumed that in the process leading to death of the patients who had suffered polytraumatism the anoxic hypoxy plays considerable role. This is supported also by the earlier data published by the institute, according to which the mortality in itself high--33.8%--of polytraumatism increases to 71.4%, if it is associated hypoxy. Consequently the aim of the therapy must be to exert the greatest activity in order to prevent or to combat, resp., the anoxic hypoxy.

Accidents↗
PubMed1975

The use of 75selenomethionine for labelling the formed and protein elements of the blood.

In the introduction the authors describe the biological role of platelets, plasma protein, fibrinogen and erthrocytes as well as the methods of tagging, separating and measuring them. The advantage of platelet labelling with 75selenomethionine is that it allows production and life-span to be determined simultaneously, however, the in vivo measurements do not give information on the site of platelet sequestration. Protein anabolism as well as katabolism can be evaluated with it, and the simultaneous determination of platelet and fibrinogen turnover may be especially useful in pathological conditions. The determination of erythrocyte life-span with 75 selenomethionine does not give so much information as the 59Fe and 51Cr methods, but when used for the examination of cell kinetics in combination with the latter methods it may afford an insight into erythropoiesis. The biological and the effective half-life periods (Teff) of 75selenomethionine are probably shorter than indicated in the literature.

Blood Cells↗