Biomedical subjects

S Okada

Publications and source records attributed to S Okada.

At least 937 records · Page 52Linked to original sources

PubMed1991

[Prednisolone Acetate Reference Standard (Control 901) of the National Institute of Hygienic Sciences].

Prednisolone Acetate Reference Standard (Control 901) for the Japanese Pharmacopoeia (JP RS) was prepared. The following analytical data were obtained: melting point 236 degrees C (decomposition); IR spectrum was same as that of JP RS of prednisolone acetate; absorptivity at 243 nm E1%lcm = 379; optical rotation [alpha]20D = +113.0 degrees; no impurity was detected by TLC, but a small amount of two impurities was found by HPLC analysis; assay by HPLC against the JP RS 100.2%. Based on the above results, this raw material was authorized as the Reference Standard of the National Institute of Hygienic Sciences.

Chromatography, High Pressure Liquid↗

PubMed1991

[Fluocinolone Acetonide Reference Standard (Control 901) of the National Institute of Hygienic Sciences)].

Fluocinolone Acetonide Reference Standard (Control 901) for the Japanese Pharmacopoeia (JP RS) was prepared. The following analytical data were obtained: melting point 268 degrees C (decomposition); IR spectrum was same as the previous JP RS of Fluocinolone Acetonide; absorptivity at 237nm E1%1lcm = 363; optical rotation [alpha]20D = 105.7 degrees; no impurity was detected by TLC, but two impurities were detected by HPLC analysis; assay by HPLC against the previous JP RS 100.2%. Based on the above results, this raw material was authorized as the Reference Standard of the National Institute of Hygienic Sciences.

Chromatography, High Pressure Liquid↗

PubMed1991

[Diclofenamide Reference Standard (Control 891) of National Institute of Hygienic Sciences].

Quality of the raw material of diclofenamide was evaluated for preparation of "Diclofenamide Reference Standard". Analytical results for the sample were as follows: melting point 240 degrees C (decomposition); UV spectrum indicates absorption maxima at 286 and 295 nm and the absorption ratio A286/A295 1.04; IR spectrum is same as that of the USP Reference Standard; no impurity was found by TLC, but a trace of one was found by HPLC analysis; the purity is assumed to be 99.95% by HPLC analysis; loss on drying 0.1%; assay by HPLC against USP Reference Standard 100.2%. Based on the results, the present raw material was authorized to be the Reference Standard of the National Institute of Hygienic Sciences.

Chromatography, High Pressure Liquid↗

PubMed1991

[Epitiostanol Reference Standard (Control 891) of National Institute of Hygienic Sciences].

The raw material of epitiostanol was examined for preparation of "Epitiostanol Reference Standard". Analytical results for the sample were as follows: melting point 130.7 degrees C (decomposition); UV spectrum indicates absorption maximum at 263 nm in ethanol; IR spectrum indicates specific absorption at 2920, 1383, 1056 and 590 cm-1; optical rotation [alpha]20D+ 24.4 degrees; no impurity was found by TLC, but a small amount of one was found by HPLC analysis and the purity is assumed to be 99.5%; water content 1.52%. Based on the results, the present raw material was authorized to be the Reference Standard of the National Institute of Hygienic Sciences.

Androstanols↗

PubMed1991

[Methoxalene Reference Standard (Control 901) of National Institute of Hygienic Sciences].

The raw material of methoxalene was examined for the preparation of the "Methoxalene Reference Standard". Analytical data obtained were as follows: ultraviolet spectrum, lambda max = 300, 249 and 218 nm; absorbance, E1cm(1%) (300 nm) = 559; infrared spectrum, same as that of USP Reference Standard of Methoxalene; melting point, 148 degrees C; thin-layer chromatography, no impurities were detected until 50 micrograms; high-performance liquid chromatography (HPLC), no impurities were detected, water, 0.04%; assay, 99.9% by absorbance method and 100.4% by the HPLC method in terms of the USP Reference Standard. Based on the above results, this raw material was authorized to be the Reference Standard of the National Institute of Hygienic Sciences.

Chromatography, High Pressure Liquid↗

PubMed1991

[Indocyanine Green Reference Standard (Control 901) of National Institute of Hygienic Sciences].

The raw material of indocyanine green was examined for the preparation of the "Indocyanine green Reference Standard (Control 901)". Analytical data obtained were as follows: ultraviolet and visible spectrum, lambda max = 785, 394, 216 nm; absorbance, E1cm(1%) (785 nm) = 3239; infrared spectrum, same as Indocyanine Green USP Reference Standard; sodium iodide, 4.1%; thin-layer chromatography, no impurities were detected until 100 micrograms; high-performance liquid chromatography, 4 impurities were detected; loss on drying, 1.8%; assay, 103.4% against USP Reference Standard. Based on the above results, this raw material was authorized to be the Reference Standard of the National Institute of Hygienic Sciences.

Chromatography, High Pressure Liquid↗

PubMed1991

[Tolnaftate Reference Standard (Control 901) of National Institute of Hygienic Sciences].

The raw material of tolnaftate was examined for the preparation of the "Tolnaftate Reference Standard". Analytical data obtained were as follows: ultraviolet spectrum, lambda max = 257 nm; absorbance, E1cm1% (257 nm) = 723; infrared spectrum, same as that of USP Reference Standard of Tolnaftate; melting point, 112.1 degrees C; thin-layer chromatography, no impurities were detected until 500 micrograms; high-performance liquid chromatography, no impurities were detected; loss on drying, 0%; water, 0.01%; assay, 100.4% by absorbance method and 100.1% by the HPLC method in terms of the USP Reference Standard. Based on the above results, this raw material was authorized to be the Reference Standard of the National Institute of Hygienic Sciences.

Chromatography, High Pressure Liquid↗

PubMed1991

[Retinol Acetate Reference Standard for Thin-layer Chromatography (Control 901) and Retinol Palmitate Reference Standard for Thin-layer Chromatography (Control 901) of National Institute of Hygienic Sciences].

The raw materials of retinol acetate and retinol palmitate were examined for the preparation of the "Retinol Acetate Reference Standard for Thin-layer Chromatography" and "Retinol Palmitate Reference Standard for Thin-layer Chromatography", respectively. Analytical data obtained were as follows: thin-layer chromatography, no impurities were detected in retinol acetate and one impurities was detected in retinol palmitate; The Rf values of retinol acetate and retinol palmitate were consistent with those of Reference Standards (Control 713), respectively; ultraviolet spectrum, lambda max = 326 approximately 327 nm; relative extinction, within the range reported in JPXI; weight variation of capsules, retinol acetate 224.0 +/- 15.5 mg (RSD 6.9%), retinol palmitate 222.0 +/- 13.8 mg (RSD 6.2%); assay, retinol acetate 57000 I.U./g, retinol palmitate 57000 I.U./g. Based on the above results, these raw materials were authorized to be the Reference Standards of the National Institute of Hygienic Sciences.

Chromatography, Thin Layer↗

PubMed1991

[Methylergometrine Maleate Reference Standard (Control 891) of National Institute of Hygienic Sciences].

The raw material of methylergometrine maleate was examined for the preparation of the "Methylergometrine Maleate Reference Standard". Analytical data obtained were as follows: melting point, 181.7 degrees C; ultraviolet spectrum, lambda max = 313 nm; absorbance, E1%1cm (313 nm) = 181.3; infrared spectrum, 3406, 2962, 2928, 1645, 1574 cm-1; thin-layer chromatography, 6 impurities were detected; high-performance liquid chromatography, no impurities were detected; loss on drying, 0.02%; optical rotation, [alpha]20D = + 44.04 degrees; assay, 99.5% against Ergometrine Maleate Reference Standard (Control 755). Based on the above results, this raw material was authorized to be the Reference Standard of the National Institute of Hygienic Sciences.

Chromatography, High Pressure Liquid↗

PubMed1991

[Cyclandelate Reference Standard (Control 901) of National Institute of Hygienic Sciences].

The raw material of cyclandelate was examined for preparation of the "Cyclandelate Reference Standard". Analytical data obtained were as follows: melting point, 60 degrees C; ultraviolet spectrum, lambda max = 252, 258 and 264 nm E1%1cm = 6.1 (252 nm), 7.5 (258 nm), 5.9 (264 nm); infrared spectrum, 3454, 2948, 1730, 1453, 1202, 737, 695 cm-1; thin-layer chromatography, no impurities were detected until 1000 micrograms; high-performance liquid chromatography (HPLC), one impurity was detected, loss on drying, 0.00%. Based on the above results, this raw material was authorized to be the Reference Standard of the National Institute of Hygienic Sciences.

Chromatography, High Pressure Liquid↗

PubMed1991

[Potassium Sucrose Octa Sulfate Reference Standard (Control 901) of National Institute of Hygienic Sciences].

The raw material of potassium sucrose octa sulfate was examined for the preparation of the "Potassium sucrose octa sulfate Reference Standard (Control 901)". Analytical data obtained were as follows: infrared spectrum, 3494, 1642, 1247, 1002, 795, 584 cm-1; high-performance liquid chromatography, 3 impurities were detected; water, 6.2%; assay of sucrose octa sulfate, 99.1%. Based on the above results, this raw material was authorized to be the Reference Standard of the National Institute of Hygienic Sciences.

Chromatography, High Pressure Liquid↗

PubMed1991

[Riboflavin Reference Standard (Control 891) of National Institute of Hygienic Sciences].

The raw material of riboflavin was examined for the preparation of the "Riboflavin Reference Standard (Control 891)". Analytical data obtained were as follows: melting point, 238.2 degrees C (decomposition); absorbance, E1%1cm = 859 (267 nm), 277 (372 nm), 327 (445 nm); infrared spectrum, same as Riboflavin Reference Standard (Control 872); thin-layer chromatography, 5 impurities were detected; high-performance liquid chromatography, 11 impurities were detected; loss on drying, 0.25%; optical rotation, [alpha]20D = -133.6 degrees; solubility, less than 16 min; assay, 100.1% against Riboflavin Reference Standard (Control 872). Based on the above results, this raw material was authorized to be the Reference Standard of the National Institute of Hygienic Sciences.

Chromatography, High Pressure Liquid↗

PubMed1991

[d-Camphor Reference Standard (Control 901) and dl-Camphor Reference Standard (Control 901) of National Institute of Hygienic Sciences].

The raw materials of d-camphor and dl-camphor were examined for the preparation of the "d-Camphor Reference Standard" and "dl-Camphor Reference Standard". Analytical data obtained were as follows: ultraviolet spectrum, lambda max = 257 nm; absorbance, E1%1cm(257 nm) = 723; infrared spectrum, 2956, 1742, 1045 cm-1; optical rotation, [alpha]20D = +42.9 degrees (d-camphor), [alpha]20D = -0.00 degrees (dl-camphor); melting point, 180 degrees C (d-camphor), 179 degrees C (dl-camphor); gas-chromatography, one impurity was detected in d-camphor and four impurities were detected in dl-camphor. Based on the above results, these raw materials were authorized to be the Reference Standards of the National Institute of Hygienic Sciences.

Camphor↗

PubMed1991

Purification and some properties of protease I having transfer action from Streptomyces griseus var. alcalophilus.

Streptomyces griseus var. alcalophilus was selected because it secreted a unique protease (protease I) that catalyzed the transfer reaction forming the hydroxamic acids of various amino acids. Protease I was purified to the electrophoretically homogeneous state and an activity of more than 125-fold that of the culture broth. The molecular weight of the enzyme was estimated to be 25,000 by gel filtration. The enzyme was most active in neutral pH for the transfer reaction forming phenylalanine hydroxamic acid, although for the hydrolytic reaction with casein as substrate it was most active in alkaline pH. The enzyme was inhibited by diisopropylfluorophosphate. Protease I catalyzed the transfer reaction synthesizing the hydroxamic acids of hydrophobic, acidic, basic, and small aliphatic amino acids such as Phe, Tyr, Leu, Asp, Glu, Arg, Lys, Ala, and Gly. These results indicate that protease I has broad donor specificity. It is also considered that protease I is a unique enzyme with transfer activity, and distinct from the alkalophilic proteinase reported previously [Yamamoto et al., Agric. Biol. Chem., 38, 37 (1974)].

Amino Acid Sequence↗

PubMed1991

Enzymatic properties of dipeptidyl carboxypeptidase from Bacillus pumilus.

Enzymatic properties of dipeptidyl carboxypeptidase (DCP) from Bacillus pumilus were investigated. The enzyme was more active on tri- and tetrapeptides than angiotensin-converting enzyme (ACE) from rabbit lung. The presence of chloride ion is essential for the hydrolysis. The Km value of angiotensin I for the enzyme was 0.119 x 10(-3) M. The enzyme was not inhibited by the mammalian ACE inhibitors lisinopril and enalaprilat. The enzyme is readily inhibited by EDTA but restored by Co2+, Mn2+, and Zn2+. Therefore, it seems to be a zinc-metallo protease.

Amino Acid Sequence↗

PubMed1991

Changes in mode of response to chemotherapy for hepatocellular carcinoma induced by transarterial embolization. A case report.

A 61-year-old man received systemic mitoxantrone chemotherapy following transarterial embolization of a large hepatocellular carcinoma with extensive intrahepatic, lung and bone metastases. His serum alpha-fetroprotein levels were 199,000 ng/ml prior to chemotherapy. He was given 10 mg mitoxantrone/m2 (14 mg/dose) intravenously every three weeks, and showed a rapid decrease in his serum levels of alpha-fetoprotein. There was almost complete resolution of the multiple bilateral metastatic lung nodules at five weeks and a marked decrease in the size of the intrahepatic metastatic nodules by seven weeks. The primary tumor, however, which had been previously treated by transarterial embolization, only underwent a 33% reduction in size according to WHO criteria. This occurred despite the disappearance, demonstrated by contrast enhanced computed tomography, of all viable tumor tissue. Ultrasonography also revealed only a minor regression, and could demonstrate no changes in the tumor echo pattern. The response pattern observed in this patient indicates the response to chemotherapy for hepatocellular carcinoma to be modified by prior transarterial embolization.

Carcinoma, Hepatocellular↗

PubMed1991

[Adrenocortical carcinoma: report of a case].

A 24-year-old man visited our hospital complaining of hypertension and headache. Endocrinological findings revealed no abnormalities except for a slight decrease in serum adrenocorticotropic hormone (ACTH), a slight increase in urine 17-ketosteroid (17-KS), and a marked increase in serum pregnenolone. Computed tomography and magnetic resonance imaging revealed a 3 x 3 cm mass in the right adrenal area and I131-aldosterol scintigraphy demonstrated a high absorption of the isotope in the right adrenal area. Vena cavography suggested a 2 x 2 cm tumor thrombus originating in the right adrenal. Under the diagnosis of the right adrenocortical carcinoma, adrenalectomy and removal of the tumor thrombus were performed. Both serum pregnenolone and urine 17-KS returned to the normal level within a week after the operation and blood pressure was well controlled without any medication 3 months after the operation. Thus, the tumor seemed to be endocrinologically active.

17-Ketosteroids↗

PubMed1991

Cell shedding from x-irradiated multicellular spheroids of human lung carcinomas.

We studied the effect of radiation on cell shedding from the surface of multicellular spheroids. Spheroids were produced from two human lung cell lines, one adenocarcinoma (LCT1) and the other small cell carcinoma (LCT2), by using a liquid overlay culture technique. The number of cells shed from both kinds of spheroids did not change significantly when they were irradiated. The number of clonogenic cells shed from both kinds of irradiated spheroids decreased sharply as the dose of irradiation increases. There were no significant differences in clonogenic cell shedding per spheroid between LCT1 and LCT2 spheroids. 400 microns spheroids were more radioresistant to inhibition of clonogenic cell shedding than 250 microns spheroids. Shed cells were more radiosensitive than spheroid cells. In these experiments, we did not obtain any results indicating that radiation enhances metastasis.

Adenocarcinoma↗