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S Okada

Publications and source records attributed to S Okada.

At least 649 records · Page 36Linked to original sources
PubMed1995

[Chromosomes, morphologic subtypes and their prognostic evaluation in acute non-lymphocytic leukemia].

Cytogenetic and morphological analyses were performed on 55 adult patients (34 males, 21 females) with acute non-lymphocytic leukemia (ANLL) diagnosed between 1986 and 1992, and the results were studied with regard to therapeutic response and prognosis. Eleven patients had M1 (20%), 14 had M2 (25.5%), 14 had M3 (25.5%), 7 had M4 (12.7%), 3 had M5 (5.5%), 5 had M6 (9.1%) and one had M7 (1.8%). The overall incidence of chromosomal abnormalities were 65.5% including 10 cases (18.2%) with t (8;21), 12 (21.8%) with t (15;17), 5 (9.1%) with pseudodiploid, 3 (5.5%) with hyperdiploid, 2 (3.6%) with hypodiploid and 4 (7.3%) with abnormalities of 5 or 7 chromosomes. Outcomes were analyzed in 45 patients. 36 patients (80%) achieved complete remission (CR). All 12 patients with M2 entered CR, and they showed a significantly longer median survival than M1. The median survival of 10 patients with t (8;21) was significantly longer than that of patients with t (15;17). However, therapeutic response and prognosis did not correlate with either chromosomal status (NN, AN, or AA) or with age-groups (> or = 60, < 60). These results confirmed that morphological subtypes and certain types of chromosomal abnormality are important variables in determining the prognosis of adult patients with ANLL.

Adolescent↗
PubMed1995

[Leprosy control].

Explore the source record for details and available documents.

Health Education↗
PubMed1995

[Scleroderma and porphyria cutanea tarda].

Examination was made of sclerodermoid changes in porphyria cutanea tarda (PCT) and PCT in association with true scleroderma. Although sclerodermoid change in PCT is rare in Japan, it is important in the diagnosis of PCT when there are few other clinical signs characteristic of PCT. In the present case, slight improvement in cutaneous sclerosis was noted following treatment for PCT, but pulmonary changes somewhat intensified. The reason for the association of PCT with progressive systemic sclerosis (PSS) remains obscure. Elevated uroporphyrin due to PCT may possibly increase cutaneous sclerosis in PSS. A diagnosis of PCT should be considered for patients presenting scleroderma, since it is difficult to distinguish sclerodermoid changes from true scleroderma.

Aged↗
PubMed1995

[MR angiography of the venous system of lower extremities with gadolinium-enhanced fast spoiled GRASS].

MR angiography (MRA) of the venous system of the lower extremities was carried out in 10 patients with varicose veins using the Gd-DTPA-enhanced Fast Spoiled GRASS (FSPGR) method. The quality of imaging of the popliteal vein (n = 20), calf deep vein (n = 20), varicose vein (n = 12), and femoral vein (n = 6) were assessed in terms of four categories: excellent, good, marginally adequate and nondiagnostic. Nineteen popliteal veins, seventeen calf deep veins, and nine varicose veins were judged as excellent or good. All femoral veins were evaluated as excellent. Enhanced FSPGR was useful for the preoperative evaluation of patients with varicose veins.

Femoral Vein↗
PubMed1995

[Studies on diagnosis of endometriosis by magnetic resonance imaging by means of fat saturation technique].

It is difficult to distinguish a small endometrial implant from adipose tissue by magnetic resonance imaging (MRI). I assessed the usefulness of fat saturated MRI in detecting small endometrial implants by comparing it with conventional MRI. Seventy-four patients with clinically suspected endometriosis were referred for MRI. In every patient, laparoscopy or laparotomy was performed. MRI was performed with a 1.5T superconducting magnet with spin echo T1, T2 and fat saturated T1 weighted images. Surgery revealed a normal pelvis in 6 patients, endometriosis in 65, and cystic lesions in three others. With fat saturated MRI, overall sensitivity, specificity, positive predictive value and negative predictive value were 87.7%, 66.7%, 95.0% and 42.9%, respectively. At surgery, 160 pigmented lesions of endometriosis were detected. Conventional MRI permitted identification of almost all ovarian endometriomas > 10mm in diameter, as with fat saturated MRI. With conventional MRI, 18 endometrial implant lesions < 10mm in diameter were detected. Including those detected by fat saturated MRI, altogether 42 lesions were detected. Conventional MRI demonstrated only 3 lesions among the 48 blueberry spots, but the additional fat saturated MRI technique increased the detection rate to 21 out of 48. Fat saturated MRI can therefore be used for detecting small endometrial implants.

Adult↗
PubMed1995

Site-specific protein synthesis in liver regeneration determined by PET.

Site-specific regeneration of the liver after 70% partial hepatectomy was investigated noninvasively in terms of protein synthesis using PET with L-[methyl-11C]methionine in a living animal. Protein synthesis in rat liver at 24 hr after partial hepatectomy did not occur uniformly in the whole liver but intensely in the middle part of the regenerating organ in comparison with the other parts. The activity was significantly low at the posterior aspect of the liver. On the other hand, site-specific protein synthesis was not observed in normal liver. These results were confirmed by bioimaging analyzer system (BAS) analysis, an invasive method that indicates radioactivities of precise intrahepatic sites. DNA synthesis in regenerating liver was also monitored with [2-14C]thymidine and analyzed by BAS 24 hr after 70% hepatectomy. Site-specific DNA synthesis in regenerating liver corresponding to the protein synthesis was also observed by BAS analysis. These results indicate that liver regeneration occurs intensely in the middle part of the liver and that PET enables noninvasive in vivo biochemical analysis.

Animals↗
PubMed1995

[A case of systemic lupus erythematosus associated with severe fibrinoid necrosis located mainly in the glomerular afferent arteriole].

We report here, a patient of systemic lupus erythematosus (SLE) with severe fibrinoid necrosis in the afferent arteriole of the glomerulus, in whom antiphospholipid antibody might have contributed to the pathogenesis. A 24-year-old female who was suffering from severe anemia with fragmented red blood cells, acute renal failure and thrombocytopenia, was admitted to our hospital. Further examinations revealed findings compatible with active lupus nephritis. Moreover, she was found to be positive for antiphospholipid antibody, and anticardiolipin antibody, as well as for lupus anticoagulant and syphilis test. Intensive treatment by methylprednisolone pulse therapy, hemodialysis, and double filtration plasmapheresis were performed. However, 13 days after admission she died suddenly because of intracranial hemorrhage. Pathological investigation of renal tissue revealed severe fibrinoid necrosis of the arterioles mainly in the glomerular afferent arteriole associated with diffuse proliferative lupus nephritis. In this case, hemolytic uremic syndrome (HUS) was associated with SLE. Antiphospholipid antibody was considered to be not only an accelerator in the arterial lesions of HUS, but also an initiator of HUS itself.

Adult↗
PubMed1995

[Association of chronic neutrophilic leukemia and myeloma with fibrillar inclusions in granulocytes].

A 57 year-old-female was incidentally found to have leukocytosis in September 1988. Physical examination revealed anemia and marked hepatosplenomegaly. Her WBC count was 33,400/microliters with 95% mature neutrophils showing toxic granules. Her neutrophil alkaline phosphatase score was 482, and serum VB12 14,600 pg/ml. Serum immunoglobulin concentrations were 582 mg/dl for IgG, 3,628 mg/dl for IgA and 48 mg/dl for IgM. IgA was determined as monoclonal origin of lambda type. Bone marrow aspiration revealed a hypercellular marrow with active granulocytopoiesis and increased plasma cells. Cytogenetic study revealed normal karyotype. The bcr rearrangement was negative for bone marrow cells. An electronmicroscopy demonstrated fibrillar inclusions in granulocytes. We diagnosed this case as a chronic neutrophilic leukemia (CNL) associated with multiple myeloma. She was treated with a course of low dose busulfan without beneficial response. She was admitted for development of huge subcutaneous hematoma of left waist in October 1990. Laboratory findings were: Hb 7.0 g/dl, WBC 55, 300/microliters, Platelets 3.3 x 10(4)/microliters, and IgA 6,607 mg/dl. She required frequent transfusions. She died of pneumonia in July 1991. The peculiar fibrillar inclusions with CNL has not been reported so far. The origin and significance of such structure remains uncertain.

Female↗
PubMed1995

Protection of neonatal kittens against feline immunodeficiency virus infection with passive maternal antiviral antibodies.

OBJECTIVE: Maternal antibodies from either vaccinated or feline immunodeficiency virus (FIV)-infected female cats (queens) were evaluated for their ability to protect kittens against homologous FIV infection. DESIGN: Kittens that received different levels of maternal antiviral antibodies from either vaccinated or infected queens were inoculated with homologous FIV at 1 week post-parturition and monitored for FIV infection. Maternal antiviral antibodies in the kittens were also measured and compared to the level of FIV infection. METHODS: Kittens at 1 week post-parturition were inoculated intraperitoneally with five median cat infectious doses of FIVPet. FIV infection was monitored by virus isolation for infectious FIV and by nested polymerase chain reaction for proviral DNA. Virus-neutralizing (VN) antibodies and antibodies to FIV transmembrane peptide and core protein were also monitored throughout the 25 weeks. RESULTS: Neonatal kittens that received high levels of antiviral antibodies from either vaccinated or infected queens were protected from FIV inoculation. Kittens that received low levels of maternal antiviral antibodies were not completely protected from similar FIV inoculation. Protection correlated more closely with the level of maternal VN antibodies than the anti-p25 antibodies transferred to the kittens. The unprotected kittens born to infected queens were not infected from vertical transmission because all littermates that were not FIV-inoculated remained free of FIV infection. CONCLUSIONS: Maternal antiviral antibodies, including VN antibodies, from either vaccinated or infected queens protected neonatal kittens from FIV inoculation. Thus, maternal antiviral antibodies play a key role in preventing or limiting infection in neonates and such antiviral immunity can be provided by vaccinated queens.

Animals↗
PubMed1995

An improved simple colorimetric method for quantitation of non-transferrin-bound iron in serum.

A simple method for direct quantitation of non-transferrin-bound iron (NTBI) in serum is introduced. NTBI was separated from serum by adding excess nitrilotriacetic acid disodium salt (NTA) to serum to form an Fe-NTA complex and then ultrafiltrated using a micro-filter. The NTBI in the ultrafiltrate was quantitated using a bathophenanthroline-based method. The optimal detection condition and several potential confounding factors were investigated. The actual measurements to samples in vivo and in vitro showed that this method is very practical.

Animals↗
PubMed1995

MR in temporal lobe epilepsy: early childhood onset versus later onset.

PURPOSE: To study the relationship between the MR findings and the clinical features in temporal lobe epilepsy in childhood (less than 10 years of age). METHODS: MR studies were performed with a 1.5-T imager on 38 temporal lobe epilepsy patients receiving drug therapy at the psychiatric department. These patients were divided into two groups according to their age at onset (10 years or less, 11 years or more). The two groups were compared in terms of the MR findings and clinical features. RESULTS: The 11 younger-onset patients included 5 with a high-signal area attributed to mesial temporal sclerosis. Clinically, all of these 5 patients had a history of "complex" febrile convulsions, which sharply distinguished them from the older-onset group. CONCLUSION: The analysis suggests that complex febrile convulsions in infancy can be associated with high-signal areas on MR attributed to mesial temporal sclerosis.

Adolescent↗
PubMed1995

[Lysosomal storage disease].

Lysosomes are the principal sites of intracellular digestion. In Lysosomes approximately 40 hydrolytic enzymes are contained. Lysosomal storage diseases are mainly caused by genetic defects that affect one or more of the lysosomal hydrolases, and result in accumulation of their undigested substrates in lysosomes, with profound pathological consequences. In this paper clinical features, diagnostic methods, and trend of the present research for lysosomal storage diseases are reviewed.

Humans↗
PubMed1995

[Krabbe disease (globoid cell leukodystrophy)].

Krabbe disease is an autosomal recessive inherited demyelinating disease, which is deficient in lysosomal enzyme, galactocerebrosidase. Pathophysiological characteristics of this disease are extreme demyelination in white matter and peripheral nerve, existence of globoid cells, absence of accumulation of main substrates, i.e. galactocerebrosidase in tissues and accumulation of psychosine. Molecular basis of this disease including isolation of a cDNA for human and murine galactocerebrosidase and cloning of genome of this gene are reviewed. The trial of gene therapy on twitcher, the mouse model of Krabbe disease, could break through on therapy on this progressive demyelinating disease.

Animals↗
PubMed1995

[Reevaluation of preneoplastic lung lesions in lung carcinoma? In bronchial carcinoma?].

Since the genetic model for colorectal carcinogenesis was presented by Fearon and Vogelstein, carcinogenesis has been thought to occur based on the accumulation of gene-mutations. In this context, preneoplastic lesions may be morphological phenotypes of different steps during multistep carcinogenesis. Historically, autopsy findings reported by Auerbach and cytological findings by Nasiell and Saccomanno support the idea that bronchial squamous metaplasia (BSM) with atypia is a preneoplastic lesion in large bronchi. Thereafter, the de novo theory became prevalent, because BSM was almost never shown to appear immediately adjacent to tiny bronchial neoplasias on examinations of resected lung materials. However, advanced molecular biological analysis shows that BSM with various grades of atypia has been found to correlate with cellular and genetic alterations (e.g., hyperproliferation, genomic instability, deletion of 3p and p53 tumor-suppressor gene etc.). These findings suggested that BSM with atypia might be a preneoplastic lesion of bronchial squamous cell carcinoma. Recently, it was shown that similar kinds of sequential genetic changes occurred in atypical adenomatous hyperplasia (AAH). It was also suggested that AAH might be a preneoplastic lesion of adenocarcinoma in peripheral regions of the lung. Based on these ideas, chemopreventive effects on BSM of folate and vitamin B12 were evaluated. The therapeutic efficacy of these vitamins was assessed in terms of grades of histopathological atypia. Vitamins were administered (B12, 750 micrograms/day and folate 10 mg/day) to 24 persons in whom bronchoscopic biopsy had revealed BSM but no malignancy. Controls consisted of 20 subjects who received no medication. Evaluation of a second biopsy performed 12 months later showed a decrease in atypia score from 1.7 to 0.4 in the treatment group but no significant change (1.4, 1.2) in the controls. At present, reevaluation of the concept of preneoplastic lesions is extremely important from the stand-point of reduction of lung cancer mortality. It is necessary to establish the criteria and therapeutic strategy for lung preneoplastic lesions.

Bronchial Neoplasms↗
PubMed1995

[Ergocalciferol reference standard (Control 941) of the National Institute of Health Sciences].

The raw material for ergocalciferol was tested for preparation of the "Ergocalciferol Reference Standard (Control 941)". Analytical data obtained were as follows: melting point, 117.6 degrees C; UV and infrared spectra, the same as those for JP Cholecalciferol Reference Standard; specific absorbance, E1%1cm = 458 (265 nm); thin-layer chromatography and high-performance liquid chromatography (HPLC), no impurities were detected, respectively; assay, 100.6% by HPLC. Based on the above results, the candidate raw material was authorized as the Japanese Pharmacopoeia Reference Standard (Control 941).

Chemical Phenomena↗
PubMed1995

[Cholecalciferol reference standard (Control 941) of the National Institute of Health Sciences].

The raw material for cholecalciferol was tested for preparation of the "Cholecalciferol Reference Standard (Control 941)". Analytical data obtained were as follows: melting point, 88.5 degrees C; UV and infrared spectra, the same as those for JP Cholecalciferol Reference Standard (Control 923), respectively; specific absorbance at 265 nm E1%1cm = 471; optical rotation, [alpha]20D = +107.3 degrees; thin-layer chromatography and high-performance liquid chromatography (HPLC), no impurities were detected, respectively; assay, 101.3% by HPLC. Based on the above results, the candidate raw material was authorized as the Japanese Pharmacopoeia Reference Standard (Control 941).

Chemical Phenomena↗
PubMed1995

[Betamethasone valerate reference standard (Control 941) of the National Institute of Health Sciences].

The raw material for betamethasone valerate was tested for preparation of the "Betamethasone Valerate Reference Standard (Control 941)". Analytical data obtained were as follows: melting point, 194.3 degrees C (decomposition); UV and infrared spectra, the same as those for JP Betamethasone Valerate Reference Standard (Control 844); optical rotation, [alpha]20D = +79.4 degrees; thin-layer chromatography and high-performance liquid chromatography (HPLC), no impurities were detected; assay, 100.0% by HPLC. Based on the above results, the candidate material was authorized as the JP Betamethasone Valerate Reference Standard (Control 941).

Betamethasone Valerate↗