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Biomedical subjects

S Oh

Publications and source records attributed to S Oh.

At least 145 records · Page 8Linked to original sources
PubMed1993

Resolution enhancement of biomagnetic images using the method of alternating protections.

Resolution of biomagnetic images using the technique of the alternating projections is proposed. Our image reconstruction procedure is divided in two steps. First, the biomagnetic inverse problem is solved by use of the projection theorem to reconstruct an initial image of the current distribution from a given magnetic field profile. Although the current distribution thus obtained has poor resolution, it can resemble the original shape of the current distribution. The second step improves the resolution of the reconstructed image by using the method of alternating projections. The procedure assumes that images can be represented by line like elements and involves finding the line like elements based on the initial image and projecting back onto the original solution space. Simulation studies were performed on a set of parallel conductors and a shape of the conductors in the form of letters, UWB@. All conductors were of line like thickness. Restored images closely resemble the original shape of the conductors.

Humans↗
PubMed1993

Pistol grip power tool handle and trigger size effects on grip exertions and operator preference.

Finger and palmar forces were measured during actual pneumatic nutrunner operation using a strain gauge dynamometer. Eighteen student subjects were assigned to one of three categories based on hand length. Two triggers and four handle spans were presented randomly. Handle span affected maximal and submaximal grip force. As span increased from 4 cm to 7 cm, average peak finger force increased 24%, peak palmar force increased 22%, and average finger and palmar tool-holding forces increased 20%. When an extended trigger was used, average peak finger force decreased 9%, peak palmar force decreased 8%, finger tool-holding force decreased 65%, and palmar tool-holding force decreased 48%. Hand size affected grip strength (MVC), grip force, and exertion level (force/MVC). Holding exertion level was maximum for large-handed subjects using a 4-cm handle and for small-handed subjects using a 7-cm handle. Subjective handle span preference increased as hand size increased. A similar experiment was performed using 11 factory workers.

Adult↗
PubMed1993

Glycosylation and high-level secretion of human tumour necrosis factor-beta in recombinant baculovirus-infected insect cells.

Human tumour necrosis factor-beta (TNF-beta) was produced in eukaryotic cells using the insect baculovirus cloning and expression system. A novel insect signal sequence, the honey-bee (Apis mellifera) prepromelittin secretory sequence, was used to aid in the post-translational modifications, glycosylation and secretion of recombinant human TNF-beta. Human TNF-beta cDNA was cloned using the insect baculovirus vector pAcC4s. Expression of the human TNF-beta was regulated by the insect Autographa californica nuclear-polyhedrosis-virus polyhedrin promoter. The 5' end of the TNF-beta cDNA was fused to the honey-bee prepromelittin signal sequence on the baculovirus vector. Insect [Spodoptera frugiperda (Sf9)] cells infected with the recombinant baculovirus secreted high levels of recombinant human TNF-beta into the culture medium. The amount of TNF-beta secreted by the Sf9 cells was estimated to be 28 micrograms of TNF-beta/ml of culture medium at 60-72 h post infection. The secreted human TNF-beta was a 22.5 kDa polypeptide which was glycosylated. Amino acid sequencing of the N-terminus of the recombinant human TNF-beta purified from the infected Sf9-cell culture confirmed that the secreted product was indeed human TNF-beta. This demonstrates that the honey-bee prepromelittin signal sequence was efficiently recognized and accurately cleaved in the Sf9 insect cells. The insect-derived TNF-beta exhibited a high cytotoxic activity similar to that of the native human TNF-beta when assessed by cytotoxic assays using murine L929 cells. Thus the insect baculovirus expression vector can be used for the production of abundant quantities of biologically active, glycosylated human TNF-beta protein.

Amino Acid Sequence↗
PubMed1992

Avian species-specific tandem repeats contain nuclear protein binding sites.

Three avian highly repetitive tandem repeats were identified and examined. These repeats had similar unit lengths (about 42 bp long) but completely different sequences each containing particular protein binding sites. Each of these repeats was found within only one of the five closely related genera studied.

Animals↗
PubMed1992

External finger forces in submaximal five-finger static pinch prehension.

Small conductive polymer force sensors were attached to the distal phalangeal pads for measuring individual finger forces exerted during submaximal static pinch. A linear force summing strain gauge dynamometer for measuring resultant five-finger pinch force was grasped vertically using a neutral wrist posture. Individual finger forces were measured at fixed total pinch force levels of 10%, 20%, and 30% of maximum voluntary exertion using pinch spans of 45 mm and 65 mm. Total pinch force and individual finger forces were also measured while similarly grasping the dynamometer and supporting fixed weights for 1.0 kg, 1.5 kg, and 2.0 kg loads using pinch spans of 45 mm and 65 mm. The index and middle fingers exerted more than 3 N greater average force than the ring and small fingers for the fixed total pinch force task. No significant individual finger force differences were observed at the 10% maximum voluntary exertion level, however both the index and middle fingers exerted more than 5 N greater force than the ring and small fingers at the 30% maximum voluntary exertion level. The average contribution of the index, middle, ring, and small fingers were 33%, 33%, 17%, and 15%, respectfully, for the fixed total pinch force task. As exertion level increased from 10% to 30%, the contribution of the middle finger was not constant increasing from 25% to 38%. Total pinch force increased from 15 N to 30 N when the load weight increased from 1.0 kg to 2.0 kg.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗
PubMed1991

Avian tandem repeats: nuclear protein binding sites and the origins of short tandem repetitive DNAS.

A 14 bp repeat isolated from the genome of a Mallard duck (Anas platyrhynchos) and a 13 bp repeat isolated from the genome of a Muscovy duck (Cairina moschata) were both tandem and genus-specific. There were 2.1 x 10(6) copies of the 14 bp repeat in the Mallard duck genome and 1.3 x 10(6) copies of the 13 bp repeat in the Muscovy duck genome. These two repeats were not obviously homologous but each contains different transcription protein binding sites. Experimentally, the two repeats were also observed to bind nuclear proteins specifically. A sequence-selective mechanism for the expansion of short tandem repeats from single ancestral sequences containing nuclear protein binding sites is presented.

Animals↗
PubMed1990

[Congenital esophagobronchial fistula communicated to esophageal diverticulum, report of a case].

We encountered a successful repair of congenital esophagobronchial fistula communicated to esophageal diverticulum. A 35 year-old-female has had frequent episodes of pneumonia since her 1st delivery. Barium esophagogram revealed a fistulous tract between the esophageal diverticulum and the right lower lobe bronchus. Bronchogram showed a ectatic dilatation at B6a and B6c. The resection of diverticulum and right lower lobectomy including the removal of fistulous tract were carried out. Histological examination of the resected fistula revealed a smooth connection between squamous epithelium and columnar epithelium. And muscularis mucosa was maintained with no evidence of inflammatory changes. So this case was diagnosed a Braimbridge I type esophagobronchial fistula. In Japan 21 cases of congenital esophagobronchial fistula with diverticulum have been reported. It is assumed that three histological findings as mentioned above is important for the diagnosis of congenital esophagobronchial fistula.

Adult↗