The degree of random orientation of excited diphenylhexatriene molecules in isolated erythrocyte membrane ghosts was investigated in order to determine the possible effect of lipid disorders on membrane structure in children suffering from type I diabetes mellitus with and without diabetic retinopathic lesions. A decrease of cholesterol in the antiatherogenic fraction HDL (1.17 +/- 0.06 in retinopathy vs 1.24 +/- 0.065 in controls) and its increase in atherogenic LDL fraction (3.88 +/- 0.23 vs 2.63 +/- 0.26) as well as developing erythrocyte membrane rigidization in diabetes and retinopathy (0.193 +/- 0.008 and 0.204 +/- 0.014 vs 0.161 +/- 0.008 in controls) were observed. Considerable fluctuations in plasma and membrane cholesterol:phospholipid ratio were most pronounced in subjects exhibiting diabetic background retinopathy. The content of membrane cholesterol compared significantly with both membrane fluidity (r = 0.677), cholesterol of LDL (r = 0.667) and cholesterol:phospholipid ratio in HDL (r = 0.693) which suggests a destructive effect of lipid disorders on cell membrane structure in diabetics.

Adolescent↗

PubMedDate not supplied

Treatment of ischaemic stroke with prostacyclin.

Ten patients with ischaemic stroke were treated with prostacyclin (2.5-5.0 ng/kg/min i.v. in 6 h courses 4-10 times during 1-2.5 days). In all patients a dramatic regression of hemiplegia, or hemiparesis, or aphasia occurred in the first few hours of prostacyclin infusion. Four to eight weeks later 6 patients left the clinic without neurological deficit; 3 patients had minor residual hemiparesis in upper limbs. In one patient, the occlusion of the contralateral carotid artery led to his death. It is considered that an antagonism may exist between endogenous cerebral prostanoids and prostacyclin and may have been responsible for the beneficial effects of prostacyclin therapy.

Brain Ischemia↗

PubMedDate not supplied

Decisions and service: seeking X.

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Consumer Behavior↗

PubMedDate not supplied

Team building.

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Job Application↗

PubMedDate not supplied

[Intraoral surgical approach to tumors destroying the axis. Report of three cases].

Primary and metastatic tumours of the axis vertebra are difficult surgical problems. The authors report 3 patients with axis tumours who underwent surgery in 1993 and 1994. Clinical symptoms and signs included pain in three, myelopathy in two, vertebrobasilar circulatory insufficiency and cranial nerve lesions in two patients. Tumours have been resected through transoral and posterior pharyngeal wall approach. The purpose of surgery was decompression of nervous and vascular structures, and histological verification of the tumour. Tumour pathology was diverse and included: multiple myeloma, chordoma and metastatic tumour from stomach. There were no postoperative complications and improvement of neurological conditions was observed.

Adolescent↗

PubMedDate not supplied

[Vertebral column primary neoplasms].

Clinical analysis was carried out in 53 cases treated surgically because of primary vertebral column neoplasms in Department of Neurosurgery in Poznań between 1976-1995. Plasmocytoma solitaire (9 cases), angioma (5 cases), osteoblastoma (4 cases) and aneurysmal bone cysts (4 cases) were observed most frequently. Neoplasms localization was as follows: cranio-vertebral junction (4 cases), cervical segment (14 cases), thoracic (23 cases) and lumbo-sacral segments (12 cases). Severe neoplastic vertebral column destruction was disclosed in 34 (64%) patients, but complete transversal spinal syndrome was noted only in 7 (13%) cases. Surgical procedures: posterior and latero-posterior approaches were applied in most cases while anterior and lateral approaches--were rarely used. In 3 cases two stage operations were performed. Intervertebral fusion or posterior autogenous graft was necessary in 39 cases.

Adolescent↗

PubMedDate not supplied

[Lumbar disk herniation treated by microsurgery].

Microsurgical technique was applied in 46 cases of lumbar disc herniation in the years 1993-1994. The group comprised 17 women and 29 men aged 17-46 years (mean age 39.54 +/- 8.72). Sciatic pain was present in 39 (85%) cases, in the remaining ones low back pain was the main symptom. Neurological deficit signs were noted in 21 (46%) cases. The authors discuss the indications to microdiscectomy, the microsurgical technique and outcomes. Microdiscectomy was carried out in cases selected strictly according to the result of neurological examination and radiological findings X-ray of lumbosacral region in 46 cases (100%), myelography in 11 (24%), CT in 4 (9%) and MRI in 31 (67%). MRI served for evaluation of signal intensity, site and site of disc hernia. In 31 cases the operation was done in patients with recognized unilateral and one-level disc hernia. Full remission of complaints was noted in 45 patients during follow-up over 2 years.

Adolescent↗

PubMedDate not supplied

[8-oxoguanosine as a marker of neoplastic process in brain].

Reactive oxygen species are toxic and cancerogenic factors to living organisms. They are suggested to cause DNA damage (modification) that triggers cancer development. It seems that oxidative damage product 8-oxo-deoxyguanosine (8-oxo-dG) which induces transversion of G to T could be a good chemical marker for cancerogenesis. The aim of our studies was to use 8-oxo-dG as a probe for brain tumour in 17 patients operated on for intracranial neoplasm. Among the patients there were 7 female and 11 male aged from 14 to 60 year. Mean age was 42.88 +/- 16.14 yrs. Several types of tumours were selected histopathologically: from neuroepithelial tissue--6 cases, meningeomas--4, metastases--3, lymphomas--2, neurinoma--1 and chondrosarcoma--1. The tumour tissue was collected from removed material and stored at -20 degrees C. DNA from the neoplastic tissues was isolated by salt method. After hydrolysis of DNA with nuclease P1 and dephosphorylation with bacterial alkaline phosphatase, the mixture of nucleosides was analysed by liquid chromatography method connected with electrochemical detector (HPLC-ECD) working at potential 400 mV. We found higher level of 8-oxo-dG in DNA of patients with malignant tumour (glioblastoma). However, at the present stage of these studies there was no proportional correlation between the level of 8-oxo-dG in DNA in tumour tissue and its malignancy.

Adjuvants, Immunologic↗

PubMedDate not supplied

[Lipid image in glioblastoma multiforme].

Changes in the content and composition of lipids in brain tumours of different degree of malignancy are still the subject of numerous scientific studies. It is known that in developing brain tumours structural and functional changes of its cells, take place, in which lipids play a crucial role. The examination of the lipid spectrum was conducted in a material extracted from tumour adjacent areas (by means of fenestration) as well as from tumours themselves in 11 patients (mean age 56.8 yrs). Based on histological studies all the tumours were qualified as glioblastoma 4th degree of malignancy. Lipids were isolated from the tumours as well as from their adjacent areas. Later on they were separated, using column chromatography and thin-layer chromatography, into three classes: phospholipids, galactolipids and neutral lipids. Separated lipids were subjected to quantitative analysis by spectrophotocolorimetric method. In comparison to adjacent areas, in tumours distinct changes of lipid levels were noticed in all analysed lipid classes. Statistical analysis showed significant decrease of phospholipids (elements stabilizing cell membranes) in tumours in comparison to their adjacent areas (Wilcoxon's test: p < 0.05). At the same time in tumours an increase of level of plasmalogens took place: phosphatidylcholine (PC) (PPC), phosphatidylethanolamine (PE) (PPE) typical elements of malignant tumours, responsible for cross cell membrane transportation processes. This phenomenon was accompanied by changes of levels of PC/PE and PC/SM (sphingomyelin) ratios connected with adhesiveness and other membrane features. Changes in the level of lipids, and phospholipids in particular, in glioblastoma 4th degree of malignancy in comparison to adjacent areas can indicate the pathological processes in cells of these tumours.

Adult↗