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Biomedical subjects

S Noguchi

Publications and source records attributed to S Noguchi.

At least 523 records · Page 29Linked to original sources
PubMed1988

Comparison of enzyme immunoassay with dextran-coated charcoal method in the determination of progesterone receptor in breast cancer cytosols.

A new enzyme immunoassay (EIA) for progesterone receptor (PR) has been developed by Abbott Laboratories. To study the correlation of the results from this new technique with the currently existing tritiated-ligand binding assay [dextran-coated charcoal (DCC) method], cytosols from 70 human breast cancers were assayed for PR by both the EIA and DCC methods. EIA showed a good reproducibility and was not susceptible to the change in protein concentration of cytosols and types of reducing agents. The correlation between the EIA and DCC methods was excellent with a correlation coefficient of 0.946; regression curve was PR (EIA) = 1.06 X PR(DCC) + 0.19 fmol/mg protein. The concordance of the results obtained from both methods was 91.4% with a cutoff value of 10 fmol/mg protein. These results demonstrate that EIA is a very useful and reliable method in the determination of PR with an excellent correlation with the conventional DCC method.

Breast Neoplasms↗
PubMed1988

Endogenous peroxidase activity in primary culture of human thyroid cells. Localization and measurement.

Intracellular localization of and an assay method for endogenous peroxidase (PO) activity were studied using primary culture of thyroid cells obtained from patients with hyperthyroidism. PO activity was visualized by cytochemical reaction and was located mainly in perinuclear cisternae and rough endoplasmic reticulum. With increased culture time, the number of cells showing positive PO activity and amount of the enzyme reaction product in individual cells showed a parallel decrease. For measurement of PO activity, cultured thyroid cells were frozen and thawed and then incubated with citric acid buffer solution containing o-phenylenediamine (opd) and hydrogen peroxide. After incubation, the optical density (OD) of the solution colorized by endogenous peroxidase was measured at 405 nm using a microplate reader. About 1 X 10(4) cells were sufficient for assay of PO activity. Using the above method to assay PO activity and sandwich enzyme immunoassay for thyroglobulin (TG), chronological changes in the PO activity and TG concentration in the culture medium were examined. Although the cells showed no decrease in number, PO activity and TG concentration decreased chronologically. When the ratio of PO activity to TG concentration was calculated, in 3 cases the ratio was almost constant, and in the remaining two, it decreased chronologically. The present biochemical method thus seems useful for determining peroxidase activity of cultured thyroid en masse.

Cells, Cultured↗
PubMed1988

Immunoelectron microscopic study of thyroid microsomal antigen.

Using human IgG F(ab') 2 prepared from pooled sera containing a high titer of thyroid microsomal antibody (TMAb), localization of thyroid microsomal antigen (TMAg) was studied electron microscopically in dissociated follicular cells obtained from 25 thyroids of patients with Basedow's disease. Twenty out of the 25 cases were positive for serum-TMAb. In 18 of these cases, the reaction product for spontaneous membrane-bound IgG was demonstrated only on the apical plasma membrane of a small number of follicular cells, but was not observed anywhere in 5 cases without serum-TMAb. After masking the spontaneous membrane-bound IgG with anti-human IgG-goat IgG Fab, deposits of the reaction product disappeared or were significantly decreased. After the masking, all cases incubated in the above-mentioned TMAb-positive IgG F(ab')2 showed an intense reaction product located linearly on the apical plasma membrane of a large number of follicular cells, but not on the lateral and basal plasma membrane and in the cytoplasm. On the other hand, after the masking, the control group incubated in human IgG F(ab')2 from a normal subject revealed the same result as that in the group with masked spontaneous membrane-bound IgG. These findings indicate that TMAg is located only on the apical plasma membrane of follicular cells.

Adolescent↗
PubMed1988

Subretinal precipitates of retinal detachments associated with intraocular tumors.

Yellowish white precipitates are sometimes observed clinically in the subretinal area of the retinal detachments. We investigated retinal detachments observed in a 47-year-old man associated with choroidal malignant melanoma and in a 1-year-old girl with retinoblastoma. Yellowish white precipitates were foam cells located around degenerated outer and inner segments of photoreceptor cells. They were more abundantly present associated with malignant melanoma than with retinoblastoma. They exhibited abundant lipid droplets, abundant lysosomes with phagocytosed materials including inner segments, fibrin, melanin and lipofuscin, abundant glycogen particles, indented nuclei, and cytoplasmic processes without intercellular junctional apparatus. They appeared to be macrophages.

Choroid Neoplasms↗
PubMed1988

A classification for intravaginal torsion of the testis.

We have developed a new classification for intravaginal testicular torsion by comparing the testes in 36 cases of intravaginal torsion with those of 30 cases undergoing castration for prostatic cancer. The classification was made by our new criteria as follows: bell-clapper type (type III) in which torsion very easily occurs; intermediate type (type II), which can be further divided into two subtypes, type IIb in which torsion easily occurs and type IIa in which torsion hardly occurs, and normal type (type I) in which torsion never occurs. We believe that our new classification is simple and useful for definite clinical understanding.

Adolescent↗
PubMed1988

[A case of duplication of the middle cerebral artery associated with arteriovenous malformation of the temporal lobe].

A rare case of duplication of the middle cerebral artery associated with an arteriovenous malformation of the left temporal lobe is reported. A 45-year-old man was admitted to our clinic on January 7, 1985 with a history of sudden onset of headache and nausea, followed by loss of consciousness for 20 minutes. Neurological signs were negative on admission. An arteriovenous malformation of the left temporal lobe fed by a duplicated middle cerebral artery and the original MCA were demonstrated on left carotid angiography. No other vascular lesion was observed on the angiograms. The arteriovenous malformation was successfully removed on January 17, 1985. Postoperative course was uneventful. Embryological relation of duplication of the middle cerebral artery to other cerebrovascular anomalies is briefly discussed.

Cerebral Angiography↗
PubMed1987

Inhibitory and stimulatory effects of glucocorticoid on androgen-induced growth of murine Shionogi carcinoma 115 in vivo and in cell culture.

It has been generally accepted for 20 years that the growth of Shionogi carcinoma 115 (SC115) is stimulated only by androgen in vivo and in cell culture. However, we recently found that the growth of SC115 is also stimulated by pharmacological, but not physiological, doses of glucocorticoid both in vivo and in cell culture and by pharmacological doses of estrogen only in vivo. In the present study, therefore, we investigated the effect of dexamethasone on androgen-induced growth of SC115 cells in vivo and in cell culture. In a serum-free medium [Ham's F-12:Eagle's minimum essential medium (1:1, v/v) containing 0.1% bovine serum albumin], the proliferation of SC-3 cells (a cloned cell line from SC115 cells) estimated by cell number and DNA synthesis reached a plateau at 10(-8) M testosterone (up to 93-fold) or 10(-6) M dexamethasone (up to 7.2-fold); high stimulation induced by higher than 10(-8) M testosterone was inhibited by the addition of 10(-5)-10(-8) M dexamethasone in a concentration-dependent manner, whereas low stimulation induced by lower than 10(-10) M testosterone was significantly enhanced by the addition of dexamethasone. The presence of typical glucocorticoid and androgen receptors in SC-3 cells was also demonstrated; dexamethasone did not bind to androgen receptor and testosterone did not bind to glucocorticoid receptor. In castrated mice, the concomitant administration of dexamethasone again significantly inhibited the high growth of SC115 tumors induced by high doses of androgen but significantly enhanced the low growth induced by low doses of androgen. The present results demonstrate both inhibitory and stimulatory effects of glucocorticoid on androgen-induced proliferation of SC115 cells in cell culture and probably in vivo.

Animals↗
PubMed1987

Nucleotide sequence of a full-length cDNA for human fibroblast poly(ADP-ribose) polymerase.

The complete nucleotide sequence of human fibroblast poly(ADP-ribose) polymerase cDNA was determined. The cDNA contains an open reading frame for a 1014 amino acid polypeptide. In the DNA binding domain of poly(ADP-ribose) polymerase, there are predicted alpha-helix-turn-alpha-helix structures and two sequences each of about 100 amino acids that are similar to each other containing potential cysteine-zinc DNA binding structures. Within the 3' untranslated region, there is an AT-rich sequence containing ATTTA, a possible mRNA destabilizer.

Amino Acid Sequence↗
PubMed1987

Transferrin as a growth factor for rat bladder carcinoma cells in culture.

Using the heterotopically transplanted rat urinary bladder system, we previously showed that normal urine has a tumor-enhancing effect on carcinogen-initiated urothelium. In an attempt to isolate a urinary growth-stimulating (tumor-enhancing) factor(s), urine was first fractionated by Bio-Gel P-100 column chromatography, and each fraction was tested for inducibility of ornithine decarboxylase (ODC) and growth-stimulatory activity in a target rat bladder carcinoma cell line, 804G. ODC inducibility was chosen as a marker for tumor-enhancing effect because it is a key characteristic of tumor promoters. There was a single peak demonstrating a strong growth-stimulatory activity as measured by [3H]thymidine incorporation. There were two ODC-inducible peaks, one located at a high molecular weight region and partly overlapped with the growth-stimulatory peak. The other was located at a lower molecular weight region. CM-Sephadex chromatography and subsequent high performance liquid chromatography successfully separated the high molecular weight-ODC activity from the growth-stimulatory activity. The latter component was found to contain transferrin (TF) by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunodiffusion with anti-rat TF antibody and was designated as urinary transferrin fraction. The urinary TF fraction and authentic rat TF stimulated growth of several rat bladder carcinoma cells maintained in a serum-free as well as a serum-deficient medium. The response was proportional to the concentration of TF ranging from 0.2 to 5 microgram/ml. Preincubation of the urinary TF fraction or TF with an anti-rat TF significantly reduced their growth-stimulatory effects in 804G cells. The high molecular weight-ODC also stimulated cell growth but to a lesser extent. These results when combined with our previous observations suggest that TF and possibly also ODC-inducible substances may be important urinary components participating in the tumor promotion by urine.

Animals↗
PubMed1987

Growth-stimulating effect of pharmacological doses of glucocorticoid on androgen-responsive Shionogi carcinoma 115 in vivo in mice and in cell culture.

It has been generally accepted for 20 years that the growth of Shionogi carcinoma 115 (SC115) is stimulated only by androgen. However, we recently found that the growth of SC115 cells is also stimulated by pharmacological doses of estrogen in vivo but not in cell culture. In the present study, the growth-stimulatory effect of glucocorticoid on SC115 cells was examined. In castrated mice, daily injections of high doses of dexamethasone (100 micrograms/mouse) markedly stimulated the tumor growth, and the growth approached that found in normal males. However, daily injections of physiological doses of dexamethasone (4 micrograms/mouse) or high doses of epitestosterone, progesterone, or cholesterol (200-5000 micrograms/mouse) did not enhance the tumor growth in castrated mice. The androgen dependency, growth speed, steroid receptors, and histological type of the tumors grown by pharmacological doses of glucocorticoid were not significantly different from those of the original SC115 tumors grown by androgen. In a serum-free medium [Ham's F-12:Eagle's minimum essential medium (1:1, v/v) containing 0.1% bovine serum albumin], the proliferation of SC-3 cells (a cloned cell line from SC115 cells) was markedly (by up to 25-fold) stimulated by 10(-10)-10(-8) M testosterone, whereas the proliferation was only slightly but significantly (by up to 3.3-fold) stimulated by 10(-8)-10(-5) M dexamethasone. The present findings demonstrate that the growth of SC115 cells in vivo and in cell culture is significantly stimulated by physiological doses of androgen or pharmacological doses of glucocorticoid.

Androgens↗