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Biomedical subjects

S Nielsen

Publications and source records attributed to S Nielsen.

At least 325 records · Page 18Linked to original sources
PubMed1987

Experimental elbow instability after transection of the medial collateral ligament.

In 12 osteoligamentous autopsy elbow preparations, the stability of the elbow was independent of the collateral ligament with flexion of less than 20 degrees and greater than 120 degrees. The anterior part of the collateral medial ligament was the prime stabilizer of the elbow in this range of motion, i.e., the flexion range of function. The maximum valgus and internal rotatory instability after transection of the medial collateral ligament, 20.2 degrees and 21.0 degrees, respectively, were found at elbow flexions from 60 degrees to 70 degrees. Selective repair or reconstruction of the anterior part of the elbow medial collateral ligament may prove to be effective in the treatment of acute or chronic elbow instability.

Biomechanical Phenomena↗
PubMed1987

Identification of two human phosphoproteins (dividin and IEF 59dl) that are first detected late in G1 near the G1/S transition border of the cell cycle.

Two-dimensional gel electrophoretic analysis (NEPHGE, IEF) of the [32P]-orthophosphate-labeled proteins synthesized throughout the cell cycle of transformed human amnion cells (AMA) revealed two phosphoproteins (dividin, Mr = 54,000, pl = 8.4; IEF 59dl, Mr = 27,000, pl = 5.7) that are present mainly in S-phase cells. These proteins are first detected at the end of G1, near the G1/S transition border, and their levels reach a maximum late in S-phase. Together with the previously identified nuclear protein cyclin, these phosphoproteins are likely candidates for proteins that may play a role in the regulation of the onset of DNA synthesis and cell division.

Amnion↗
PubMed1986

Bone strength measurements at the proximal tibia. Penetration tests and epiphyseal compressive strength.

Three penetration tests were obtained from corresponding locations at each condyle of 19 proximal tibiae. The patterns of condylar cancellous bone strength varied little between knees. The medial condyle was the strongest with an average medial to lateral strength ratio of 1.9, and in most knees the medial condyle was strongest centrally while the lateral condyle was strongest posteriorly. The penetration strength at 5 successive 2 mm levels beneath the resected subchondral surface also showed a constant pattern of variation. Bone strength decreased significantly at first except at the posterolateral site, then tended to level off. The reduction of strength was most pronounced in the centre of the condyles. The penetration tests were good predictors of the static compressive strength of the proximal tibia. Correlation coefficients of approximately 0.90 were obtained indicating statistically highly significant correlations (p less than or equal to 0.00001). The tests were carried out with equipment developed for in vivo measurements of cancellous bone strength during total knee replacement. The findings confirm the close relationship of these investigations to conventional compression tests.

Aged↗
PubMed1986

The static stabilizing function of the popliteal tendon in the knee. An experimental study.

We investigated the importance of the anterior cruciate ligament (ACL), its two components (the AMP and PLP), and the lateral compartment ligaments with special attention to the popliteal tendon (PT) in relation to valgus-varus, axial rotation, and anterior-posterior instability. Mobility patterns were drawn from 15 osteoligamentous knee preparations after successive transection of the structures. Even when combined lesion involved the lateral collateral ligament (LCL), the PT, and the posterolateral capsule (PLC), anterior tibial displacement was not effected until the ACL was cut. The lateral structures acted as secondary restraints to anterior displacement, and prevented simultaneous anterolateral rotation during application of the anterior force. LCL and PLC transection affected varus stability in full extension, and instability improved on additional section of the PT, even if the ACL remained intact. Maximum instability after combined lateral lesion occurred at 40 degrees of flexion, whether the ACL was transected or not. Further flexion caused a rapid decrease of instability. The PT was shown to effectively restrain varus instability from 0 to 90 degrees of flexion. It was experimentally established with regard to axial rotation that marked posterolateral instability was impossible with an intact PT. Maximum instability was recorded at 30 degrees of flexion after combined lesion to the lateral structures. Even in the extension, considerable posterolateral instability was observed. The restraining effect of the PLC was prominent in extension.

Biomechanical Phenomena↗
PubMed1986

Posterior instability of the knee joint. An experimental study.

We investigated the importance of the posterior cruciate ligament (PCL) and the medial and lateral compartmental structures for translatory and simultaneous axial rotatory instability in 25 osteoligamentous knee preparations. Instability was registered continuously from 0 degree to 90 degrees of flexion with application of a constant force to the tibia. Isolated transection of the PCL increased the posterior tibial displacement with flexion to a maximum of 10 mm at 90 degrees of flexion; when combined lesions to the lateral structures were included, the popliteal tendon (PT) in particular turned out to have a major secondary stabilizing function. The posterior tibial displacement in flexion was doubled when all lateral structures were included in the lesions. Transection of the PCL and all the medial structures led to a notable increment in posterior displacement increasing with flexion. Major increments in simultaneous tibial rotation were recorded only after combined lesions to either medial or lateral structures. A reverse pivot shift was provoked after combined lateral lesions when the PT was included. Even an anteromedial subluxation was released after lesions to the medial structures. Regardless of the type of lesion, the specimens remained stable concerning anterior-posterior displacement in extension. No changes in the anterior tibial displacement were observed.

Biomechanical Phenomena↗
PubMed1986

Nuclear patterns of cyclin (PCNA) antigen distribution subdivide S-phase in cultured cells--some applications of PCNA antibodies.

Immunofluorescence studies using PCNA autoantibodies specific for the proliferation-sensitive protein cyclin have revealed dramatic changes in the nuclear distribution of this protein during the S-phase of normal and transformed cells. Patterns of cyclin antigen distribution subdivide S-phase and have provided new cell cycle landmarks. Some of these (nucleolar exclusion or staining), mimic topographical patterns of DNA synthesis thus arguing for a role of this protein in some specific aspect of DNA replication. Cells outside S-phase (G0 included) stain only weakly with PCNA antibodies, stressing the usefulness of this reagent for identifying proliferating cells (S-phase cells) of both normal and malignant origins.

Animals↗
PubMed1986

Proliferation-sensitive nuclear phosphoprotein "dividin" is synthesized almost exclusively during S phase of the cell cycle in human AMA cells.

Studies of the polypeptides synthesized by normal and transformed human cultured cells under a variety of physiological conditions have revealed a basic 54-kDa protein NEPHGE 10a, whose rate of synthesis is sensitive to changes in the rate of cell proliferation. This nuclear phosphoprotein, which we have termed "dividin" (present only in populations of cells committed to divide), is synthesized almost exclusively during the S phase of the cell cycle of transformed human amnion cells (AMA). Dividin synthesis is first detected late in G1 near the G1/S transition border, reaches a maximum late in S phase, and declines thereafter. As expected for an S phase-specific protein, no detectable synthesis of dividin was observed in growth-arrested normal human cultured cells of epithelial and fibroblast origin. These findings suggest a role for this protein in events leading to DNA replication and cell division.

Amnion↗
PubMed1986

Monoclonal antibody specific for human nuclear proteins IEF 8Z30 and 8Z31 accumulates in the nucleus a few hours after cytoplasmic microinjection of cells expressing these proteins.

A monoclonal antibody (mAB 1C4C10) that reacts specifically with human nuclear proteins IEF 8Z30 and 8Z31 (charge variants; HeLa protein catalogue number; Bravo, R., and J. E. Celis, 1982, Clin. Chem., 28:766-781) has been microinjected into the cytoplasm of cultured cells that either express (primates) or lack these proteins (at least having similar molecular weights and pIs; other species), and its cellular localization has been determined by indirect immunofluorescence. Nuclear localization (nucleolar and nucleoplasmic) of the antibody was observed only in cells expressing these antigens, suggesting that a determinant present in IEF 8Z30 and 8Z31 is required for cytoplasm-nuclear translocation. Nuclear migration was not inhibited by cycloheximide, implying that these proteins may shuttle between nucleus and cytoplasm. The results assumed to support the signal rather than the free diffusion model are further supported by microinjection experiments using antibodies (proliferating cell nuclear antigen/cyclin, DNA) that react with nuclear components but do not recognize cytoplasmic antigens. Furthermore, they raise the possibility that some nonnuclear proteins may be transported to the nucleus by interacting with proteins harboring nuclear location signals.

Animals↗
PubMed1986

Handling of lysozyme in isolated perfused proximal tubules.

Isolated, perfused proximal tubules from rabbit were used to study the luminal endocytic uptake, digestion, and transcellular transport of 125I-lysozyme. Ten tubules were perfused for 20 min with 125I-lysozyme and [14C]inulin and then with tracer-free perfusate for additional 40 min before fixation. The uptake and digestion of lysozyme was calculated per millimeter tubule length. The transfer of intact lysozyme from perfusate to the bath was measured and compared with the transfer of inulin. Five tubules were processed for electron microscope autoradiography, and the grain distribution was analyzed quantitatively. The results show that 2.7% of the perfused amount of lysozyme was taken up, and 21.3% of the absorbed protein was digested. The present experiments demonstrate that the transfer of intact lysozyme from lumen to bath is not significantly different from the transfer of inulin. The autoradiographic analysis showed that lysozyme was localized mainly in endocytic vacuoles and lysosomes after 60 min of perfusion.

Animals↗
PubMed1986

Anterior and posterior shoulder instability. A cadaver study.

In a cadaver study of 10 glenohumeral joint specimens, the anterior and posterior displacement of the humeral head was recorded after cutting parts of the rotator cuff and capsular structures applying a constant force to the humerus. The posterior structures were important for anterior stability in the first 40 degrees of abduction. Anterior subluxation was changed to luxation in the first half of abduction, but only after lesions to the anterior part of the rotator cuff and upper half of the anterior capsule. For posterior displacement, the posterior part of the rotator cuff was found significant from 0-90 degrees of abduction, and the posterior capsule between 40 degrees and 90 degrees of abduction. The anterior part of the rotator cuff and the upper part of the anterior capsule were essential in the first 40 degrees of abduction. Cutting the capsular structures only, we found that the entire anterior capsule resisted anterior displacement for 70-90 degrees of abduction, and the entire posterior capsule from 50-90 degrees of abduction. For posterior displacement, the entire posterior capsule was important from 60 to 90 degrees of abduction. Clinically, a large lesion to the posterior structures seems to be essential for any major anterior displacement, and posterior displacement leading to subluxation only seems possible in connection with a major anterior injury.

Biomechanical Phenomena↗
PubMed1986

Posterior instability of the shoulder. A cadaver study.

In a cadaver study of 15 shoulder specimens, the internal rotation of the joint was measured applying a constant internal torque of 1.5 Nm to the humerus. The specimens were suspended with the medial border of the scapula in vertical position. A lever fixed to the humerus was fitted with strain gauges for measurement of internal torque and sensors for measurement of internal rotation at different degrees of abduction from 0-90 degrees. Cutting the teres minor and infraspinatus muscle tendons increased internal rotation in the first 40 degrees of abduction. Internal rotation was further increased in this range by cutting also the proximal half of the posterior capsule. Lesion to the posterior capsular structures alone increased internal rotation from 40 degrees of abduction. In conclusion, among the posterior structures of the shoulder joint, the teres minor and the infraspinatus muscle tendons stabilize the joint for internal rotation in the first half of abduction, and the lower half of the capsule in the last part.

Biomechanical Phenomena↗
PubMed1985

The posterior cruciate ligament and rotatory knee instability. An experimental study.

The importance of the posterior cruciate ligament in relation to valgus-varus and axial rotatory stability in the knee joint was investigated. Mobility patterns were drawn from 20 osteoligamentous preparations after successive transection of the posterior cruciate ligament (PCL), the medial and lateral collateral ligaments, and the posterior joint capsule. The knee joint remained grossly stable after isolated transection of the PCL, and further cutting of either one of the collateral ligaments or of the posterior capsule yielded no greater instability than one should expect from isolated cutting of each of these structures. The posterior cruciate ligament was the stabilizing factor in flexion and external rotation after injury to the lateral collateral ligament and the posterolateral capsule, and it restricted internal rotation after cutting of the medial cruciate ligament and the posteromedial capsule. Valgus instability was markedly increased during the whole range of movement when PCL was included in injury to the medial compartment ligaments, and when included in a lateral compartment injury a further varus instability was found, though only in the flexed or semiflexed knee. No hyperextension could be demonstrated after these injuries.

Biomechanical Phenomena↗
PubMed1985

Total condylar knee arthroplasty. A report of 2-year follow-up on 247 cases.

Two hundred and forty-seven consecutive total knee replacements using the Insall-Burstein standard total condylar knee system with 2 years' follow-up were studied. Of the 238 available to follow-up, two were reoperated during the period, owing to infection and traumatic loosening of a tibia component respectively. Seventy-nine patients were operated for rheumatoid arthritis and 94 for osteoarthritis. In assessment of the total therapeutic result, registration of pain, walking ability, range of motion, muscle strength, flexion deformity, valgus-varus deformities, instability, and use of walking aids were included, according to a rating system. Excellent or good results were found in 91.5%; 6.5% were fair, and 2% were poor. There were two cases of infection; one resulted in removal of the prosthesis and an arthrodesis was done. There were four suspected aseptic loosenings, three cases of transient peroneal nerve palsies, and three patients who developed reflex dystrophy.

Adult↗
PubMed1985

Experimental distal subluxation in the glenohumeral joint.

In an experimental set-up including ten shoulder specimens, increments in the acromiohumeral distance (the subacromial space) were measured on an X-ray radioscope after the vertical stabilizing structures of the glenohumeral joint had been cut. It was found that when only the supraspinatus tendons were cut, the acromiohumeral space only increased by a few millimeters. When first the coracohumeral ligament and then the proximal one-third of the anterior capsule were cut, the acromiohumeral distance was doubled in each case. Distal subluxation did not occur when only the anterior capsule was cut. When the coracohumeral ligament was also cut, the acromiohumeral distance was more than doubled. It is concluded that the most important structures in the pathogenesis of distal subluxation are first the coracohumeral ligament and then the proximal part of the glenohumeral capsule.

Acromion↗
PubMed1985

Evaluation of growth in anorexia nervosa from serial measurements.

Measured longitudinal growth data in a sample of 66 patients with anorexia nervosa (AN) (58 females and 8 males) is compared to expected growth from normative standards. The data was subject to cross-sectional as well as longitudinal analysis. Females had a normal growth rate and pattern, in particular a normal sequence and timing of pubertal events. Distribution of weight for age indicated a slight (non-significant) overweight before onset of anorexia nervosa. Weight by height was significantly above the expected before onset of AN. Mean age at Peak Height Velocity (PHV) was 11.5 yr. Mean size of PHV 8.03 cm/yr. A subsample of females (those with onset of AN after menarche) had a mean age of menarche of 12.88 yr. Mean distance in time from age at PHV to age at menarche was 1.34 yr. These results are normal for Scandinavian girls. Male anorexics were tall for age, heavy for age and, where a PHV could be seen (5 out of 8 cases), had signs of early puberty. Mean age at PHV was 11.8 yr, a highly significant difference from the normal mean age at PHV which is 14.06 yr for Scandinavian boys. Weight by height was distributed as in the reference population, that is, with no indication of pre-morbid obesity for male probands.

Adolescent↗
PubMed1985

Transtubular transport of proteins in rabbit proximal tubules.

The purpose of the present experiments was to study possible different pathways of intracellular transport of proteins after luminal and basolateral uptake in isolated rabbit proximal tubules. Tubules were exposed to cationized ferritin (CF) in the perfusion fluid and horseradish peroxidase (HRP) in the bath simultaneously or to HRP in the bath alone for 30 min. The peritubular fluid (bath) and perfusion fluid were then exchanged and the tubules either fixed immediately or allowed to function during chase-periods for 10, 20, 30, or 60 min before fixation to follow the migration of the proteins through the cells. The proteins were to a large extent found separated in different vacuoles and lysosomes at all time periods studied, indicating separate pathways after uptake via the luminal and basolateral membranes respectively. About 0.5% of the CF taken up by the cells was transported through the cells and became located in the intercellular spaces. HRP was transported from the peritubular fluid to the apical cytoplasm of the tubules indicated by a gradual accumulation of small HRP-containing vesicles, first in the basal part of the cells and then in the apical cytoplasm. In tubules perfused with both CF and HRP in the perfusate, the CF and HRP were found together in apical vacuoles and lysosomes. After perfusion with HRP alone, this tracer was found in similar large vacuoles and lysosomes in the apical cytoplasm, in contrast to the small HRP-filled vacuoles seen after uptake from the bath.

Animals↗