Six monoclonal antibodies (mAbs, T-2.1, 2, 3, 4, 5, 6) which react with a trichothecene mycotoxin, T-2 toxin (T-2), were prepared. All antibodies specifically reacted with T-2 but less (0.5% of T-2) with the metabolites such as HT-2 toxin and 3'-hydroxy-T-2 toxin. Significant but less than 0.02% cross-reactivity was observed with T-2 triol, 3'-hydroxy-HT-2 toxin and neosolaniol. No significant reaction with other trichothecenes such as deoxynivalenol, nivalenol, fusarenon-X, crotocin, or roridin A was observed. The least detectable amount of T-2 with the best mAb T-2.1 was 2.5 pg T-2 per assay. This specific and highly sensitive assay for T-2 was applied for the quantitation of T-2 in wheat flour spiked with mycotoxin, with combination of a simple extraction procedure.

Animals↗

PubMedDate not supplied

Antitumor effect of S-1 on DMH induced colon cancer in rats.

The aim of this study was to establish an autochthonous colon cancer model in the rat as an in vivo secondary screen for the general evaluation of new anticancer agents against colorectal cancer, and also to evaluate practically the antitumor activity of 1M tegafur-0.4M 5- chloro-2,4-dihydroxypyridine-1M potassium oxonate(S-1), a new p.o. fluoropyrimidine. Thirty-two Sprague-Dawley rats received dimethlhydrazine(40 mg/kg) s.c. once weekly for 10 weeks to induce colon cancer.20 weeks after beginning the carcinogen treatment, a barium enema was performed to visualize tumors. The animals were divided into a control group and S-1 treatment group. After 5 weeks of treatment, the barium enema was repeated. The mean doubling time of 24 tumors in the control group was 19.0 + 8.4 (SD) days. Response to S-1 was judged as effective when the doubling time exceeded 35.8 days, calculated from the mean + 2SDs in the control group. The response rate of S-1 was 55%, 34% of the tumors were decreased in size after treatment. This figure was higher than that of clinically-used 5-fluorouracil(5-FU) derivatives; 5-FU;6%, Tegafur(FT):6%, 1M tegafur-4M uracil(UFT):14%, reported in our previous study. An autochthonous colon cancer model is useful to evaluate the clinical therapeutic efficacy of drugs for colorectal cancer, and S-1 is expected to have a high therapeutic effect on human colorectal cancer.

1,2-Dimethylhydrazine↗

PubMedDate not supplied

Primary malignant lymphoma of the duodenum: report of a case.

We present a case of long-term survival in primary malignant lymphoma of the duodenum treated with a curative surgical resection and post-operative chemotherapy. A 72 year-old man was admitted to our hospital complaining of epigastric discomfort. Endoscopy revealed an ulcerative tumor in the duodenal bulb. The tumor was diagnosed histologically as malignant lymphoma by endoscopic biopsy. A distal gastrectomy and duodenal bulb resection were performed with lymph nodes dissection. The tumor was histologically classified as B-cell phenotype, large cell, immunoblastic lymphoma of high grade, according to the Working Formulation. The patient received CHOP (cyclophosphamide, doxorubicin, vincristine and prednisone) chemotherapy after the operation, and he has no sign of recurrence during a follow-up period of 6 years.

Aged↗