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Biomedical subjects

S N Wang

Publications and source records attributed to S N Wang.

25 records · Page 2Linked to original sources
PubMed1994

[Comparison on the kidney nourishing and yang strengthening functions of three different species of herba Cistanches].

Oral administration of water extraction of three kinds of herba cistanches can increase the weight of young mice, prolong the duration of swimming and promote anoxia tolerance in mice. These water decoctions can also increase the body weight, the activities of SOD in serum and the lifespan of "Yang insufficient" model mice induced by administration of hydrocortisone acetate.

Animals↗
PubMed1990

A role for the metal binding domain in determining the DNA sequence selectivity of Fe-bleomycin.

Previous studies of Fe-bleomycin-mediated DNA cleavage have established that the bithiazole moiety + C-terminal substituent of bleomycin are required for DNA binding, while the metal binding domain is responsible for O2 activation. Although recent studies have indicated that the metal binding domain also participates in DNA unwinding, and in determining the sequence and strand selectivity of DNA cleavage, no study has defined the structural domain that bears primary responsibility for the observed pattern of bleomycin-mediated DNA degradation. Presently, by the use of four synthetic analogs of bleomycin demethyl A2 having the functional domains connected by rigid spacers of varying lengths, the source of DNA cleavage specificity has been determined. When the four analogs cleaved 242- and 127-base pair 5'-32P-end-labeled DNA restriction fragments containing isolated Fe-bleomycin cleavage sites, all four produced cleavage at the same preferred sites. Because the (oligo)glycine spacers altered the distance between the domains by as much as 14 A, the identical cleavage patterns argue that the primary determinant of sequence specificity for these analogs is the metal binding domain.

Base Sequence↗
PubMed1988

The synthesis of protective fragments of alpha-human atrial natriuretic polypeptide (alpha-hANP).

In this paper the synthesis of alpha-human atrial natriuretic polypeptide fragments (positions, 1-4, 5-10, 11-16, 17-22, 23-28) by solution method is reported. In the synthesis of some peptides, the results from mixed anhydride procedure and active ester procedure are compared. In the synthesis of other peptides, the effects of reaction temperature, basicity of catalyst, and reaction time on the specific rotations and yields of these compounds are observed. The sequence of amino acids of all the protective polypeptides is confirmed by FAB-MS.

Atrial Natriuretic Factor↗
PubMedDate not supplied

Pathophysiologic glomerulotubular growth factor link.

Circumstantial evidence from clinical and pathologic correlations in patients with glomerular diseases and proteinuria suggest that glomerular protein ultrafiltration contributes to tubulointerstitial injury. A series of studies was performed to examine the hypothesis that in rats with adriamycin-induced nephropathy or with diabetic nephropathy (but not in normal rats) high molecular wt. growth factors are ultrafiltered into tubular fluid and act on tubular cells through apical membrane receptors. Analysis of proximal tubular fluid that was collected by nephron micropuncture indicates ultrafiltration of IGF-I, TGF-beta and HGF. Respective receptors are also expressed in apical membranes in some parts of the nephron as examined by immunohistochemistry. In vitro cell culture experiments using proximal tubular fluid obtained from rats with experimental glomerular diseases indicate that ultrafiltered IGF-I may contribute to increased distal tubular Na-absorption. Indirect evidence also suggests that this growth factor may increase the secretion of collagen types I and IV in proximal tubular cells. TGF-beta and HGF cause increased expression and basolateral secretion of MCP-1 in proximal tubular and collecting duct cells. There may be other biologic effects on tubules that are caused by apical exposure to ultrafiltered growth factors. These studies suggest that the glomerular ultrafiltration of bioactive proteins causes or contributes to tubulo-interstitial pathology in glomerular proteinuria.

Absorption↗
PubMedDate not supplied

[Educational effects of a short-term diabetes camp for children].

A Diabetes camp provides children with fun and educational experience. Seventeen diabetic school children (12 boys and 5 girls) participated in a short-term diabetes camp from April 16 to 17, 1988. Their ages ranged from 8.5 to 13.2 years with an average of 11.3 +/- 1.5 (mean +/- SD) years. The duration of their diabetes varied from 0.05 to 5.3 years with an average of 2.7 +/- 3.3 years. The camp staff consisted of a pediatric endocrinologist, 3 nurses, 4 dietitians, 3 social workers, 4 assistant diabetes educators, and 2 counsellors who were specialized in music and recreational activities. The children's abilities in managing their diabetes were assessed by using a scoring system before and after the camping. Wilcoxon matched-pairs signed-rank tests with significance levels set at p < 0.05 (two-tailed) were conducted to determine whether there were any significant changes in the results from pre- and postcamp assessments. The children showed significant improvement in using glucose-monitoring devices, drawing insulin, injecting insulin, and choosing the right exchange of food with the p values of less than 0.05, 0.01, 0.01, and 0.01 respectively. We concluded that this short-term diabetes camp was educational for school children with diabetes. The campers improved their knowledge and skill in management of diabetes.

Adolescent↗