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Biomedical subjects

S N Cherenkevich

Publications and source records attributed to S N Cherenkevich.

At least 37 records · Page 2Linked to original sources
PubMed1985

Chemiluminescence of luminol caused by interaction of cytochrome P-450 and cytochrome C with cumene hydroperoxide: comparative studies.

A comparative study of the interaction of cumene hydroperoxide with cytochrome P-450LM2 and with cytochrome C has been undertaken using the chemiluminescence method in the presence of luminol. Considerable differences in the mechanisms of action of both hemoproteins have been revealed with various inhibitors of radical stages, i.e. superoxide dismutase, mannitol, sodium azide, and alpha-tocopherol. It is shown that molecular oxygen participates in the process of hemoprotein-catalyzed hydroperoxide oxidation of luminol.

Animals↗
PubMed1985

[Peroxidase activity of the influenza virus].

The appearance of chemiluminescence has been detected in influenza virus-luminol-hydrogen peroxide system. An effective decrease of its intensity in the presence of oxygen radical scavengers (sodium azide, superoxide dismutase, mannitol, ethanol) is indicative of activated oxygen taking part in the reaction. Increased luminescence in the presence of o-phenanthroline and its suppression by antimycin A suggest that peroxidase-like activity of influenza virus is due to the presence in it of Fe-S-protein.

Animals↗
PubMed1985

[Changes in the activity of hydrogen ions in lymphocytes during the endocytosis of concanavalin A].

Changes of hydrogen ions activity in rat thymocytes were studied during the process of concanavalin A (Con A) endocytosis by cells. A possibility was shown to use fluoresceinisothiocyanate-labeled Con A for measuring pH values in the sites of mitogen localization. It is established that during the action of Con A endocytotic vesicles with low pH value are formed, while the pH value of the cytoplasm is increased. The pH value measured in the sites of mitogen localization depends on the treatment of cells by alpha-methyl-D-mannoside and sodium azide. Mechanisms of acidic-basic equilibrium changes in the cytoplasm of lymphocytes upon cell stimulation with mitogens are discussed.

Animals↗
PubMed1984

[Chemiluminescence of thrombocytes in thrombin-induced aggregation].

Chemoluminescence induced by thrombin and aggregation of thrombocytes as well as effect of aspirin on the reactions were studied. Intensity of thrombin-induced chemoluminescence correlated with accumulation of malonic dialdehyde and hydroperoxides in cells. Chemoluminescence of thrombocytes appears to occur after the thrombin stimulation of cyclooxygenase pathway of arachidonic acid oxidation in cells.

Arachidonic Acid↗
PubMed1983

[pH-dependent conformational changes in thrombocyte proteins].

A study was made of the medium pH influence on structural states of platelets by optical methods. Within the pH range (6-8), two pH induced reversible changes of platelet state were observed. A conclusion is made that the structural rearrangements in platelets induced in the medium by changes in hydrogen ion concentration may involve some rearrangements in platelet proteins, and thus acting as a factor regulating platelet function.

Blood Platelets↗
PubMed1982

Changes in membrane fluidity associated with lymphocyte stimulation by succinyl-concanavalin A.

Time-resolved fluorescence anisotropy studies of human peripheral blood lymphocytes labeled with 1,6-diphenyl-1,3,5-hexatriene were carried out at temperatures between 4 and 38 degrees C. For unstimulated freshly-isolated lymphocytes the calculated order parameters were found to be 0.62 at 4 degrees C and 0.44 at 37 degrees C. Mitogen-induced alterations in the order parameter were evident within minutes after addition of succinyl-concanavalin A to the cells, increasing to a value of 0.56 after 2 h at 37 degrees C. Both stimulated and unstimulated cells show a decrease in fluorescence anisotropy over the next 2-3 days of culture and after the third day the time-resolved fluorescence anisotropy decay profiles of the two populations of cells were indistinguishable. Our results indicate that there are both short- and long-term changes in the membranes of the cell upon stimulation by mitogen.

Adult↗
PubMedDate not supplied

[Damage to erythrocytes caused by the interaction of nitrite-ions with hemoglobin].

The formation of two hemoglobin forms (methemoglobin and nitrite methemoglobin) in native human erythrocytes in the presence of sodium nitrite in suspension was shown. In normal erythrocytes, the interaction of intracellular oxyhemoglobin with nitrite ions results in the formation of methemoglobin, whereas in metabolically exhausted erythrocytes, this leads predominantly to the formation of nitrite methemoglobin. The nitrite methemoglobin reacts with hydrogen peroxide to form reactive intermediates (e.g. peroxynitrous acid) and the products of hemoglobin destruction. During the storage of erythrocyte suspensions containing methemoglobin and modified nitrite methemoglobin, differences in the forms of erythrocytes and the degree of their hemolysis were revealed. It is assumed that the formation of methemoglobin leads to the destruction of erythrocytes.

Erythrocytes↗
PubMedDate not supplied

[Regulation of the neurite growth].

Recent achievement of molecular biology and neurophysiology resulting in remarkable progress in the understanding of processes of neurodifferentiation, playing the relevant role both in an ontogenesis and working of adult brain. New representations have been formed about a role of cytoskeletal proteins in mechanisms of neurites growth. In the review we discussed recent data regarding mechanisms of neuronal differentiation regulation. The special interest is given to a problem of influence of electrical fields on neuronal growth and differentiation. The mechanisms of signal transduction from a cell surface to nuclear are in detail analyzed. The classification of the physical and chemical factors of growth neurites regulation is given.

Animals↗
PubMedDate not supplied

[Consumption of intracellular hydrogen peroxide in epithelial human amnion cells].

The method for determination of intracellular oxidants content has been proposed. The method is based on the analysis of changes of 2,7-dichlorodihydrofluorescein oxidation. The theoretical model of hydrogen peroxide diffusion and its consumption in the cell and quantitative determination of hydrogen peroxide gradient through the cellular membrane has been built taking into account the experimental data. The coefficient for H2O2 permeability through the plasma membrane of human amnion cells is (2.1 +/- 0.1) x 10(-4) cm x c(-1).

Amnion↗
PubMedDate not supplied

[Photodamage of gram-positive and gram-negative bacterial cells in the presence of chlorin e6 derivatives].

The kinetics of photoinduced damage sensitized by chlorine e6 and its derivatives was studied in Escherichia coli and Bacillus subtilis. The effectiveness of E. coli photoinactivation in the presence of chlorines was 100-200 times lower as compared with that of B. subtilis. The structural organisation of bacterial cell walls apparently played an essential role in the penetration of tetrapyrrole pigments into the cell and in their binding.

Bacillus subtilis↗
PubMedDate not supplied

[The chlorine e6-photosensitized inhibition of human thrombocyte aggregation].

ADP-induced aggregation of platelets in the presence of chlorin E6 under the action of visible light depending on the pigment concentration and the time of light action was studied. It was shown that the photosensitized effect of chlorin E6 on the platelet-enriched plasma results in platelet aggregation inhibition.

Adenosine Diphosphate↗