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Biomedical subjects

S N Chatterjee

Publications and source records attributed to S N Chatterjee.

234 records · Page 13Linked to original sources
PubMedDate not supplied

Induction of lambda prophage by furazolidone.

A dose-dependent prophage induction by furazolidone exhibited a gradual rise to a maximum, corresponding to an exposure dose of 1.2 microgram/ml X h and a gradual fall thereafter. A 2-3-fold higher level of induction was achieved when the lysogens were treated with furazolidone in the presence of a metabolizing mixture. A maximum of about 70% efficiency of induction was achieved. Kinetics of prophage induction by any concentration of furazolidone exhibited a common pattern, viz., an initial rise for 15-20 min, then a plateau extending up to about 60 min and a faster rise thereafter. Higher concentrations of the drug (10 micrograms/ml) exhibited a toxic effect. Chloramphenicol at a concentration of 20 micrograms/ml inhibited the furazolidone-induced prophage induction, the plaque-forming units gradually decreasing from several minutes after the chloramphenicol treatment. The burst size of the lysogens was not significantly affected by treatment with 2 micrograms/ml of furazolidone up to a period of about 10 min, but thereafter, decreased faster with the duration of furazolidone treatment. The "latent period' of induction decreased linearly with the duration of furazolidone treatment.

Bacteriophage lambda↗
PubMedDate not supplied

On the inhibitory activities of a new boron compound and ultrasound against the mouse ascites tumour.

The inhibitory effects of a new boron compound, dihydroxy (oxybiguanido) boron (III) hydrochloride monohydrate (HB), and ultrasound (US) of a frequency 25 kHz on the growth of ascites tumour in female Swiss mice were studied by monitoring the survival, weight of tumour-associated material, tumour cell count, serum alkaline phosphatase activity and the haematological parameters of the treated animals. 5-Fluorouracil (5-FU), a well-known anticancer agent, was used as positive control. While HB exhibited a very significant antitumour action, US alone produced a small but significant inhibitory effect. The combination of US with HB or 5-FU produced an extra antitumour action as compared to the actions of these chemicals used singly. The mechanisms of action of the new boron compound (HB) and US are discussed.

Alkaline Phosphatase↗
PubMedDate not supplied

Ultrastructure of isolated basement membranes in the acellular human renal cortex.

Transplant quality human kidneys were sliced (approximately equal to 2 mm thick) and cortical regions further minced to approximately 1 mm3. These samples were treated successively with EDTA and detergents to solubilize cellular materials but leave the extracellular matrix (ECM), including basement membranes (BMs) intact. Resultant acellular cortices bear a remarkable resemblance to their in vivo counterparts. By light microscopy, patent, expanded tubular BM (TBM), peritubular capillary BM (PTCBM) and Bowman's capsule BM (BCBM) are surrounded by a swollen ECM which is occasionally fibrillar. Glomerular BM (GBM) lacks supporting interstitium but nevertheless remains convoluted and does not collapse. At the level of transmission electron microscopy, the diverse morphological characteristics of each BM type are evident. Random thickness measurements (lamina densa only) show that BCBMs are thickest (approximately equal to 2,400 nm) followed by TBMs (approximately equal to 750 nm), GBMs (approximately equal to 335 nm) and PTCBMs (approximately equal to 125 nm). Power transformations to normalize right-sided skew of thickness distribution curves reduce their arithmetic means 3-16%. SEM studies confirm LM and TEM observations that isolated GBMs maintain their free-standing spheroidal shapes and indicate that they may be intrinsically rigid. Moreover, their surface topographical details are preserved. We conclude that the acellular cortex offers a clarified view of renal interstitial morphology and demonstrates structural diversity among major renal BM types. Moreover, it provides a baseline of morphological information on which to assess pathological conditions of renal cortical extracellular matrix.

Basement Membrane↗
PubMedDate not supplied

Effect of sodium cholate on the phase transition temperature of dipalmitoyl phosphatidylcholine.

On treatment with sodium cholate, the phase transition temperature (Tc) of multilamellar liposomes derived from dipalmitoyl phosphatidylcholine (DPPC) progressively decreased with increasing cholate: lipid-P molar ratio. A molar ratio of 4.5 caused a one degree depression in Tc. The unilamellar cholate vesicles of DPPC exhibited the same Tc as that of the multilamellar ones. The formation of unilamellar vesicles from the multilamellar ones by cholate treatment was investigated by electron microscopy.

Cholic Acid↗
PubMedDate not supplied

Electron microscopic study of the polymyxin treated goat erythrocytes.

Polymyxin B produced dose dependent changes in the surface topography of the goat erythrocyte cells. Transformation from the normal biconcave discs through crenated structures to the final rounded or spherical shape was recorded by scanning electron microscopy. A maximum of three to four crenations per cell was recorded corresponding to a polymyxin dose of 15.62 micrograms/ml. Transmission electron microscopy of the ultrathin sections of treated or untreated erythrocytes indicated that the crenations were formed by protrusions of the plasma membrane, occurring presumably because of the local increase of membrane fluidity after polymyxin treatment. Changes in the shape of the erythrocytes to the ultimate rounded forms were also indicated by the transmission electron microscopy.

Animals↗
PubMedDate not supplied

Thermal stability of DNA interacting with furazolidone and Cu(II) ions.

Furazolidone, on complexing with DNA, led to its thermal stabilization. The increase in transition temperature of DNA (delta Tm) increased linearly with % A--T content. Increasing concentration of Cu(II) ions progressively lowered the transition temperature of DNA, but Cu(II) ions were not equally effective in lowering the transition temperature of furazolidone-DNA complex. When equimolar amounts of Cu(II) ions and furazolidone were used, the stabilisation effects of furazolidone prevailed over the destabilisation effect of Cu(II) ions.

Animals↗