Search PubMed⌕ Search

Biomedical subjects

S Murai

Publications and source records attributed to S Murai.

At least 127 records · Page 7Linked to original sources
PubMed1987

Ultrastructural and immunocytochemical characterization of polymorphonuclear leukocytes from gingival crevice in man.

Polymorphonuclear leukocytes from the gingival crevicular fluid (CF-PMNs) of patients with generalized severe periodontitis were examined using electron microscopy and immunocytochemical techniques. CF-PMNs were found to contain numerous phagocytic vacuoles. This suggests that CF-PMNs actively phagocytized various substances from the environment. Immunocytochemical staining with FITC-conjugated IgG, IgM, and IgA reagents and TRITC-conjugated C3 reagent was applied to CF-PMNs as well as peripheral blood PMNs incubated with cell-free crevicular fluid. The cytoplasm of PMNs exhibited numerous granular foci of immunofluorescence. This finding suggests that these proteins were acquired from the environment by PMNs. The coincidental appearances of immunoglobulins and C3 in a single cell were considered to be immune complexes phagocytized by CF-PMNs in generalized severe periodontitis.

Adult↗
PubMed1985

Glycylprolyl dipeptidylaminopeptidase from Bacteroides gingivalis.

Dipeptidyl aminopeptidase activity was found in the culture medium of Bacteroides gingivalis 381. The enzyme, hydrolyzing glycylprolyl-4-methylcoumaryl-7-amide, was purified 750-fold from culture medium by ammonium sulfate precipitation, Sephadex G-200 gel filtration, and DEAE Bio Gel A column chromatography. The molecular weight, determined by gel filtration, was approximately 160,000. The isoelectric point of the enzyme, estimated by isoelectric focusing using polyacrylamide disk gel electrophoresis, was about pH 6.2. The optimum pH of the enzyme was about 8.0, and the Km value was 0.05 mM. The enzyme activity was strongly inhibited by phenylmethylsulfonylfluoride and diisopropylfluorophosphate. The purified enzyme specifically cleaved glycylprolyl dipeptide from partially digested type I collagen.

Bacteroides↗
PubMed1985

[A simply designed apparatus and its use for measuring vertical and horizontal motor activities in methamphetamine-treated mice].

A simple apparatus using the infrared ray, which can divide spontaneous motor activity (SMA) of mice into two behavioral components, a vertical movement (VM) and a horizontal movement (HM) was designed. VM and HM were evaluated by counting the number of times that infrared rays were blocked by the mouse in the activity cage [300(H) X 260(D) X 130(W) mm] with 9 infrared photo-couplers for measuring VM and one infrared photo-coupler for measuring HM. When methamphetamine (MAM) was subcutaneously administered to mice pretreated with saline (0.1 ml/10 g, sc) for 3 days, VM and HM were as follows: 1) At 0.1 mg/kg of MAM, the same level of activity was observed as with saline. 2) At 1.0 mg/kg of MAM, increased activity was noted. 3) At 10 mg/kg of MAM, a marked increase was noted, showing more enhancement of HM than VM, which had developed into a stereotyped behavior. In summary, this new apparatus was found capable of dividing the SMA of mice into the two behavioral patterns of VM and HM, and providing their measurements.

Animals↗
PubMed1984

Stimulation of serotonin synthesis in rat brain after antiepilepsirine, an antiepileptic piperine derivative.

Piperine and two of its derivatives, antiepilepsirine (AE or 3,4-methylendioxycynnamoylpiperine) and compound 7448 (N-isopropyl 3 (4 chloro-phenyl) propenoylamide) are very effective in stimulating serotonin (5HT) synthesis. AE raises the ratio of free-to-bound tryptophan (TP) in plasma and induces a long-lasting increase of this aminoacid in brain. At the same time in striatum and limbic area it causes a lasting increase in 5 hydroxyindolacetic acid (5HIAA) a 5HT metabolite and to a lesser extent, an increase in the levels of the monoamine itself. Together with this action on 5HT metabolism we found that AE caused release of 3H-5HT from an in vitro synaptosomal preparation. It thus appears that piperine and its derivatives AE and compound 7148 affect the central serotonergic system.

Animals↗
PubMed1984

Effect of ipratropium bromide on nasal mucociliary transport.

Ipratropium bromide is a parasympatholytic drug. After application to the nose, the nasal mucociliary transport time was measured using the method of the saccharin test. This compound did not cause any changes in the nasal mucociliary transport function.

Administration, Intranasal↗