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Biomedical subjects

S Moriya

Publications and source records attributed to S Moriya.

At least 91 records · Page 5Linked to original sources
PubMed1985

[On the effect of an oocyte factor on the formation of cumulus oophorus in human antral follicles].

The possible existence of a granulosa cell growth factor (GGF) in the oocyte was investigated by using the mitotic index (%) of granulosa cells (GC) in human antral follicles. 50 follicles (0.4-13 mm in diameter) in 35 ovaries obtained from 35 women were used for the experiments. In each follicle, GC were divided into two groups, cumulus and mural GC, according to the distance from the oocyte. The following results were obtained: The Mitotic Index (MI) of cumulus GC was 8.2 +/- 0.5 (M +/- SE) and was two fold higher than that of mural GC, 4.7 +/- 0.4 (p less than 0.001). As follicles developed, MI of mural GC increased until their diameter reached 3mm but beyond 3mm, MI decreased. On the other hand, there was little relationship between MI of cumulus GC and the follicular size, and MI was constantly high in value. MI of both cumulus and mural GC was higher in the late follicular phase than in other phases of the cycle. These results suggest that the proliferation of GC in growing follicle might be regulated by some unknown substance (GGF) secreted from the oocyte as well as the endocrinological environment. It is also suggested that the presence of the oocyte plays a crucial role in the cumulus oophorus formation in the antral follicles.

Adult↗
PubMed1984

[Reevaluation for overlooked signs of cervical smears in cases of uterine cervical adenocarcinoma].

Though the incidence of adenocarcinoma is not high, this cancer has been a matter for discussion on the grounds that mass screening frequently shows "false negative" cases. The purpose of this study was to clarify the factors which had caused a misdiagnosis through the re-investigation of patients' previous cervical smears. In spite of continuous smear tests at various intervals, there was a long delay in the diagnosis, ranging from 6 months to 5 years, in 14 patients out of 51 cases of cervical adenocarcinoma treated in our hospitals during the past 6 years. In 12 of these 14 cases a careful re-examination of previously overlooked smear preparations revealed definite signs of adenocarcinoma in most cases (86%). A cause of the delay in diagnosing adenocarcinoma seems likely to be related to cytological inspections rather than to the process of taking smears. Characteristics overlooked signs in the misdiagnosed smears were either "rosette-like" arrangements or "irregular clusters of sheet-like" arrangements. The rosette-like arrangements a pattern indicative of cervical adenocarcinoma, were found in 10 of the above cases (71%). The findings obtained suggest that for the diagnosis of cervical adenocarcinoma, cytological diagnosis by smear could be made even in the early pre-clinical stage and when cervical adenocarcinoma is suspected on a cytological basis in this stage, conization is also strongly recommended despite the punch biopsy.

Adenocarcinoma↗
PubMed1983

Structure and organization of rRNA operons in the region of the replication origin of the Bacillus subtilis chromosome.

Structure and organization of two complete ribosomal RNA (rRNA) gene sets, rrnO and rrnA, were determined for the first time in Bacillus subtilis. They are located at the region of the replication origin of the chromosome. Each set constitutes a single operon of: two tandem promoters - leader sequence - 16S rRNA gene - Ile-tRNA gene - Ala-tRNA gene - 23S rRNA gene - 5S rRNA gene - termination signal. The first promoter (P1) of rrnO differs from that of rrnA in sequence and function. P1 of rrnO was used very little for transcription either in vivo or in vitro while P1 was predominantly used in rrnA. A putative transcript of the entire operon was determined and constructed into a secondary structure. Analysis of in vivo transcripts by S1 mapping revealed primary processing sites at the loop and stem structure of 16S rRNA in rrnO and rrnA. A unique sequence in the leader region of rrnO can be formed into a highly complexed secondary structure and affects processing of mature 16S rRNA. The sequences of the two spacer tRNA genes are highly conserved between B. subtilis and Escherichia coli.

Bacillus subtilis↗
PubMed1980

[Influence of oocytes upon proliferation of cultured granulosa cells (author's transl)].

Granulosa cells (G-C) collected from mature follicles of human and bovine ovaries were cultured with an oocyte from mature follicles of human, bovine and porcine ovaries to investigate the influence of the oocyte upon the proliferation of G-C as well as the differentiation of G-C. For this purpose, G-C were compared in mitotic index (MI) between those cultured with oocyte and without oocyte control at the fourth day of culture. 1) G-C cultured with an autogenous oocyte--A bovine oocyte cultured with bovine G-C increased in MI 2.8 times compared with the control. Human G-C cultured with a human oocyte also appeared to raise the MI 2.7 times compared with the control. 2) G-C culture with heterogeneous oocyte--Moreover, a porcine oocyte cultured with bovine G-C acted to increase the MI to 2.7 times that of the control. An addition of a porcine oocyte to human cultured G-C also almost doubled the MI as compared with that of the control. 3) Within 2 days after culture of G-C with an auto- or hetero-genetic oocyte from which the cumulus oophorous had been removed, the oocyte was surrounded by a dense mass of small type of G-C, which resembled follicular G-C morphologically and indicated a high value of MI. The cells on the periphery from the oocyte, however, were clearly enlarged differing from those close to the oocyte and began to resemble lutein cells as the culture days went on. 4) Instead of the oocyte, sterilized muscle, cartilage and bone tissues were cultured with the G-C as controls. However, no binding of G-C with these tissues were found as was verified between G-C and the oocyte. These results suggest that the oocyte may characteristically bind with G-C to accelerate the multiplication and inhibit the luteinization of G-C.

Animals↗