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Biomedical subjects

S Morimoto

Publications and source records attributed to S Morimoto.

At least 577 records · Page 32Linked to original sources
PubMed1987

Immunoreactive parathyroid hormones in the circulation and cerebrospinal fluid from patients with renal failure: possible restriction of parathyroid hormone by the blood-brain barrier.

Parathyroid hormone is reported to be a possible causal factor of abnormalities of electroencephalograms of patients with renal failure. In this study, the parathyroid hormone levels were compared in the circulation and cerebrospinal fluid of seven normal subjects and 22 patients with renal failure including those who showed abnormal electroencephalograms. The circulating levels of both C-terminal and N-terminal parathyroid hormone in the subjects studied showed a positive correlation (C-terminal, r = 0.58, P less than 0.01; N-terminal, r = 0.61, P less than 0.01) with the grade of abnormality of the electroencephalogram. However, the levels of C-terminal and N-terminal parathyroid hormone in the cerebrospinal fluid of both normal subjects and patients with renal failure were below the detectable limit (C-terminal, less than 0.1 ng/ml; N-terminal, less than 2.3 pg/ml). These data suggest that in patients with renal failure, the effect of parathyroid hormone on the central nervous system is mediated in some other way than via the cerebrospinal fluid.

Adult↗
PubMed1987

[Relationships between distribution of disorganized cardiac muscle cells and disease state in hypertrophic cardiomyopathy].

Congestive heart failure is rare in hypertrophic cardiomyopathy (HCM). Therefore, disorganization of the cardiac muscle cells, a histological hallmark of HCM, has not been associated with reduced ventricular contraction. We performed a clinicopathological study of 18 patients with HCM to determine whether disorganization contributes to dilatation and impaired contraction of the ventricles, resulting in affecting the disease condition. The subjects were classified into Group A (11 patients) without dilatation of the ventricular cavities after formalin fixation at autopsy (left 11.9 ml and right 19.1 ml), Group B (two patients) with dilatation of the left but without of the right ventricular cavity (left 31.9 ml and right 25.6 ml), and Group C (five patients) with biventricular dilatation (left 38.6 ml and right 36.8 ml). Various parameters were compared among the three groups. The mean age of the patients was 44.0 years for Group A, 18.5 for Group B and 10.2 section for Group C. The mean cardiothoracic ratio was 51.8% (n = 7) for Group A, 53% (n = 1) or Group B, and 74.0% section for Group C. Obstruction of the left ventricular outflow tract was observed in 100% (n = 8) of the Group A patients, in 0% section of the Group B patients, and in 20.0% section of the Group C patients. The mean left ventricular ejection fraction was 81.0% (n = 5) in Group A, 65.5% in Group B and 39.0% section (n = 2) in Group C. None of the Group A patients had chronic congestive heart failure, whereas all Group C patients died of this condition. Using transverse and longitudinal tissue sections from the ventricles, and a quantitative method employing light microscopy, we determined the area of the myocardium characterized by disorganization and interstitial fibrosis. Septal disorganization was observed in 67.4%, 83.4% and 91.1% section of the transverse sections in Groups A, B and C, respectively, and in 17.0%, 24.9% and 63.5% section of the longitudinal sections in the respective groups. As for the left ventricular free wall, disorganization involved 21.2%, 69.4% section and 90.8% section of the transverse sections and 15.9%, 34.9% section and 38.1% section of the longitudinal sections in Groups A, B and C, respectively. In the right ventricular free wall, there was disorganization in 17.7%, 20.4% and 84.3% section of the transverse sections in 12.4%, 11.5% and 57.0% section of the longitudinal sections in the respective groups.(ABSTRACT TRUNCATED AT 400 WORDS)

Adolescent↗
PubMed1987

Elevation of tissue cathepsin B and L activities in gastric cancer.

Cathepsin B and L activities were determined with 7-amino-4-methyl-coumarin conjugates as substrates using small tissue specimens of gastric cancer and benign gastric ulcer. Both cathepsin B and L activities were higher in homogenates of gastric cancer tissue than in regenerative mucosa of benign gastric ulcer. Cathepsin B activity was much higher in cancer tissue than in tissue surrounding the tumor. In contrast, the activity in regenerative mucosa of benign gastric ulcer was not elevated as compared with the tissue surrounding the ulcer. Similar results were obtained with cathepsin L activity. These results indicate that cathepsin B and L are related to proliferation and evolvement of gastric cancer.

Adenocarcinoma↗
PubMed1987

Primary pulmonary hypertension. Study of eight autopsy cases.

Eight autopsy cases of primary pulmonary hypertension were studied. All cases were diagnosed as primary plexogenic arteriopathy. Microscopical study of the lungs showed in every case hypertensive arterial changes of Grade II-VI after Health & Edwards' criteria. Truncal pulmonary artery of three cases revealed aneurysm or severe dilatation with thrombus, and dilation was also recorded in another three cases. Histology of gross pulmonary artery in several cases presented disruption and defect of elastic fiber and alcian-blue positive microcystic change in media, lymphoid cell infiltration in outer media and inner adventitia, adventitial thickening, and intimal fibrosis. The liver of 7 cases showed secondary congestive changes and in one case intrahepatic portal vasculitis.

Adolescent↗
PubMed1987

Diuretic effect induced by intrarenal infusion of pinacidil in anesthetized dogs.

The diuretic action of pinacidil, a new vasodilating antihypertensive agent, was studied using anesthetized dogs. Intrarenally administered pinacidil (0.4, 2.0 and 10 micrograms/kg/min) produced a dose-related increase of renal blood flow, without altering mean arterial pressure. The glomerular filtration rate significantly increased only with the highest dosage. Pinacidil caused a dose-related augmentation of urine formation and, when given at 10 micrograms/kg/min, increased urine flow and urinary excretion of electrolytes to about 10 times and 3 to 9 times over the respective control value. In comparison, hydralazine (4.0 and 20 micrograms/kg/min) produced no significant diuretic action and bradykinin, given at a dose (0.02 micrograms/kg/min) equipotent to pinacidil (10 micrograms/kg/min) with respect to increasing renal blood flow, was less effective. Pinacidil administration significantly increased the fractional excretion of phosphate. Thus, pinacidil-induced diuresis seems to be due mainly to the vasodilating effect on renal vessels, with some contribution from an inhibitory effect on sodium reabsorption in the proximal tubules.

Anesthesia↗
PubMed1987

[Primary hyperparathyroidism in 23 cases].

Twenty-three cases of primary hyperparathyroidism were experienced at Wakayama Medical College Hospital and affiliated hospitals between January, 1969 and December 1985. Histopathological findings were adenoma in 22 cases, hyperplasia in 1 case. On the clinical diagnosis of this disease, the most sensitive laboratory test was serum calcium level, which was elevated in 21 patients (91%), and in 87.8% of all samples measured. Serum ionized calcium and phosphorus were also sensitive measures. The positive rate of serum PTH was 67% in 18 cases. Tl-chloride scintigraphy identified preoperatively the site of the adenomas in 8 out of 10 cases, and computed tomography in 10 out of 15 cases. Both methods are non-invasive, and are beneficial for the preoperative examination of the localization of parathyroid adenoma. After parathyroidectomy, serum calcium and ionized calcium levels recovered rapidly within the normal limit in 12 hours. On the other hand, serum phosphorus level increased gradually up to the normal limit in about 1 week.

Adenoma↗
PubMed1987

[Uroepithelial tumors of the upper urinary tract following bladder cancer].

Five hundred and nineteen patients with primary bladder cancer were treated between January, 1969 and December, 1984, 12 of whom had developed upper urothelial tumors. These patients had received various transurethral treatment for the primary bladder lesions, except for one patient who had undergone total cystectomy and ileal conduit diversion. Overall incidence of patients with upper urinary tract tumors following bladder cancer was 2.3%. The incidence of patients with treated bladder tumors (13.2%) for dye workers was higher than that for the general population (1.1%). The interval between initial treatment of the bladder tumor and diagnosis of the upper tract tumor ranged from 7 to 170 months (mean 70 months). The incidence of upper tract tumors increased with the passage of time. We conclude that the occurrence of upper urinary tract tumors following primary bladder cancers is promoted by nonspecific chemical irritants against the urothelium already made unstable by certain urinary chemical carcinogens.

Aged↗
PubMed1987

[Treatment of a case of childhood acute non-lymphocytic leukemia (ANLL) using high-dose cytosine arabinoside for intensification of early therapy].

A 12-year-old girl with acute non-lymphocytic leukemia was treated with a protocol involving high-dose cytosine arabinoside (Ara-C) for intensification of early therapy. The patient, who had been revealed to have CNS infiltration on admission, achieved complete remission after receiving ACMA/BHAC combination and intrathecal MTX. As an early intensification treatment, ID/HD Ara-C was safely and effectively administered; this consisted of ADR (45 mg/m2 iv) Day 1, intermediate-dose Ara-C (0.5 g/m2, 1-h drip, q. 12 h) Days 2-4, and high-dose Ara-C (3 g/m2, 3-h drip, q. 12 h) Days 10-11, followed by L-asp (6,000 U/m2, im) on Day 12. Ten months later, the patient has been in continuous complete remission. High-dose Ara-C should be included with caution as an early intensification of treatment to improve the therapeutic results of childhood ANLL.

Aclarubicin↗
PubMed1986

Effect of fibronectin and von Willebrand factor on the adhesion of human fixed washed platelets to collagen immobilized beads.

The adhesion of human fixed washed platelets (FWP) to collagen was measured using collagen immobilized beads. The addition of normal plasma or severe von Willebrand disease (VWD) plasma to FWP decreased the adhesion, suggesting the presence of some inhibitors of platelet adhesion in human plasma. Although the adhesion of FWP in severe VWD plasma was not different from that of FWP in normal plasma, the addition of purified von Willebrand factor (vWF, 1-2 mu/ml ristocetin cofactor) to FWP in buffer increased the FWP adhesion at higher flow rates, and the percent of adhesion in the absence of vWF was 10% (collagen 500 micrograms) and 30% (collagen 1,000 micrograms) of that in the presence of vWF at 10 ml/min. The enhancing effect of the vWF on FWP adhesion was also observed by pretreatment of the collagen column with vWF suggesting the important role of bound vWF to the collagen; adhesion 72% to the collagen column (1,600 micrograms) treated with vWF and 16% to the collagen column without the pretreatment at 10 ml/min. The promoting effect of vWF was also present in some commercial factor VIII preparations which had no large or intermediate multimers of vWF antigen. The adhesion of FWP was inhibited by fibronectin (FN) and the binding of ristocetin cofactor (vWF:RCo) to collagen fiber was also inhibited by FN; bound vWF:RCo to 50 micrograms/ml collagen in the absence or presence of 125 micrograms/ml FN were 60% and 8% respectively. It is suggested that vWF, even small multimer of vWF:Ag, is involved in the initial platelet-collagen interaction at high flow rates, while plasma FN acts as one of anti-adhesion factor.

Adult↗
PubMed1986

Purification and characterization of rat urinary esterase A1.

An enzyme, esterase A1, which hydrolyzes tosyl-arginine methyl ester (Tos-Arg-OMe) was separated from esterase A2 and kallikrein of male rat urine and purified by a procedure involving ammonium sulfate fractionation, ion exchange chromatography, hydrophobic chromatography and gel filtration. The resulting preparation was apparently homogeneous, as assessed by polyacrylamide gel electrophoresis. The molecular weight of the preparation was estimated to be 27,000 by SDS-polyacrylamide gel electrophoresis and 30,000 by gel filtration. The enzyme was more specific for arginine methyl esters than for lysine methyl esters. The optimum pH determined with Tos-Arg-OMe as a substrate was 8.0 and the Km was 11.8 mM. The Tos-Arg-OMe esterolytic activity of esterase A1 was inhibited by soybean trypsin inhibitor, but not by aprotinin. In immunodiffusion analysis, the antiserum to esterase A1 formed immunoprecipitin arcs with this enzyme and the urine collected from rat bladder, but not with esterase A2, kallikrein, plasma and the urine collected from ureters. These results indicate that rat urinary esterase A1 differs from esterase A2 and kallikrein. The esterase A1 appears to be produced by accessory sex glands and excreted via the spermiduct into the urine.

Animals↗
PubMed1986

Identification of some polycyclic nitrogen-containing compounds in coal-derived oil.

A systematic method for the identification of aza-arenes in coal-derived oil was developed. The basic nitrogen-containing substances were extracted with 6 M hydrochloric acid and fractionated sequentially by using gel chromatography and thin-layer chromatography (TLC) on an alumina plate. The aza-arenes in these fractions were separated by using glass capillary gas chromatography. Individual compounds in the column effluent were trapped in a system consisting of a valve for flow switching and a trapping tube made from a glass capillary. The fluorescence spectra of nanogram to subnanogram amounts of trapped compounds were measured. Some attempts were made to identify components based on their TLC RF values and their fluorescence spectra, in addition to their mass spectra.

Aza Compounds↗
PubMed1986

The different effects of exogenous and neuronally released norepinephrine on renin release in rat kidney cortical slices.

The effects of veratrine on renin release from rat kidney cortical slices were compared with those of norepinephrine (NE). Veratrine (10-100 micrograms/ml) produced a concentration-dependent increase in renin release. This action was markedly attenuated by propranolol but not influenced by prazosin. On the other hand, relatively higher concentrations of NE produced an inhibition of renin release and the inhibitory action was reversed by prazosin. These results suggest that exogenous NE inhibits renin release by preferential activation of alpha 1-adrenoceptors while neuronally released NE stimulates renin release by exclusive activation of beta-adrenoceptors.

Animals↗