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Biomedical subjects

S Mor-Yosef

Publications and source records attributed to S Mor-Yosef.

At least 55 records · Page 3Linked to original sources
PubMed1988

Antigen presenting cells in human decidual tissue: III. Role of accessory cells in the activation of suppressor cells.

Human decidual antigen presenting cells (DAPCs) exposed to fetal cells in vitro induce generation of suppressor T cells among a population of peripheral blood lymphocytes. Human lymphocyte antigen (HLA)-class II positive antigen presenting cells were isolated from early normal human decidual tissue and from peripheral blood (PAPCs) by adhering Ficoll-Paque separated cell suspensions to fibronectin. In contrast to PAPCs, DAPCs pulsed with fetal antigens induced a radio-sensitive, Leu 1,2-positive T suppressor cell population. A nylon wool adherent B cell population is required during the in vitro induction of the suppressor cells. These suppressor cells impair primary mitogen and mixed lymphocyte culture (MLC) responses, generation of anti-trinitrophenyl (TNP) cytotoxic T lymphocytes, and antibody response of autologous and allogeneic lymphocytes. Only intact viable embryonic cells can effectively confer upon DAPCs the ability to induce T suppressor cells. The T suppressor cell induction by DAPCs primed with fetal antigens is restricted by the major histocompatibility complex. Our results show that the HLA-DR molecules are the most prominent restriction elements.

Adult↗
PubMed1987

Antigen presenting cells in human decidual tissue. II: Accessory cells for the development of anti-trinitrophenyl cytotoxic T lymphocytes.

Antigen presenting cells were isolated from early human decidua or peripheral blood by elution with EDTA of cells that after Ficoll-Paque separation bear receptors for, and have bound to, fibronectin. Decidual antigen presenting cells (DAPCs) co-cultured with TNP-modified autologous T cells induced the development of cytotoxic anti-TNP T lymphocytes with an efficiency comparable to that of peripheral blood antigen presenting cells (PAPCs). Treatment with anti HLA-class II antibody plus complement and UV radiation resulted in substantial inhibition of the accessory cell function. The T cell mediated lysis of TNP-modified targets is restricted by the major histocompatibility complex. Our results show that HLA-class I molecules are the most prominent restriction elements. The relevance of these data to the immunological mechanisms operating at the feto-maternal interface is discussed.

Adult↗
PubMed1986

Anencephaly and the nature of fetal response to vibroacoustic stimulation.

The fetal response to vibroacoustic stimulation has been used in recent years as a measure of well being. Vibroacoustic stimulation theoretically may evoke both tactile and auditory responses in the fetus. In clinical practice, it is important to distinguish which type of response is actually activated. An answer may be suggested through observation of two anencephalic fetuses who demonstrated no response to such stimulation. Neuroanatomic considerations are discussed that suggest which auditory mechanisms are involved in the fetal response to vibroacoustic stimulation.

Acoustic Stimulation↗
PubMed1986

Antigen-presenting cells in human decidual tissue.

The responses of peripheral blood human T lymphocytes supported by decidual antigen-presenting cells (DAPCs) to a variety of immunogenic stimuli were studied and compared to those of T cells supported by peripheral blood antigen-presenting cells (PAPCs). Antigen-presenting cells were isolated from early normal decidual tissue or peripheral blood by elution with ethylenediamine tetraacetic acid of cells that after Ficoll-Paque separation bear receptors for all have bound to fibronectin. DAPCs pulsed with soluble or particulate antigens induced proliferation of T cells with an efficiency equivalent to PAPCs. Decidual tissue APCs also showed the ability to stimulate auto- and alloreactivity. Treatment with anti-human lymphocyte antigen (HLA) class II antibody and ultraviolet radiation resulted in substantial inhibition of the accessory cell function of DAPCs as well as of PAPCs. Bromodeoxyuridine and light treatment of alloreactive T cells generated in vitro was used to demonstrate that DAPCs primed with a synthetic polypeptide antigen (T,G)-A-L can stimulate only HLA class II-compatible T lymphocytes.

Adult↗
PubMed1985

Antepartum diagnosis of monoamniotic twin pregnancy.

A case of monoamniotic twin pregnancy complicated by preeclampsia is presented. Early diagnosis was achieved at the 16th gestational age by ultrasonography. The pregnancy was terminated at term by a cesarean section. Both twins survived with excellent outcome. The importance of antepartum diagnosis of this rare condition is discussed.

Amnion↗
PubMed1985

Fetal movements in hypertensive pregnancies.

The maternally perceived fetal movements counts of 273 hypertensive patients were compared to normal controls and their degree of fetal activity was related to perinatal outcome. Pregnancy induced hypertension was associated with fetal activity which was significantly lower than controls at the beginning of the third trimester and significantly higher at term. In cases of chronic hypertension patients, 8 had cessation or marked reduction of fetal movements to less than 4 in 12 hours, and all had poor fetal outcome. The remaining 265 hypertensive patients had a 92% incidence of good fetal outcome. Markedly reduced fetal movements in hypertensive patients are highly suggestive of fetal distress and following verification by additional tests the appropriate clinical measures should be undertaken.

Female↗
PubMed1984

Acute feto-maternal transfusion--diagnostic considerations.

A sinusoidal fetal heart rate pattern followed by severe deceleration was encountered in a fetus at 39 weeks' gestation during antepartum fetal nonstress testing. The only indication for fetal monitoring was a maternal complaint of cessation of fetal movements. The abdominally-delivered newborn was found to be severely anaemic and the cord was tightly wrapped round its body. A pathophysiological mechanism for massive feto-maternal transfusion is suggested. Various laboratory tests were utilized to identify the condition, quantitate the amount of transfusion, and estimate the time of its occurrence. Globin synthesis studies in maternal blood indicated that a significant fetal blood loss had occurred within 24 hours of delivery. The significance of these findings as applied to the clinician is discussed.

Acute Disease↗
PubMed1984

Antepartum fetal heart rate pattern associated with major congenital malformations.

Antenatal fetal heart rate monitoring of 20 fetuses with major congenital malformations revealed loss of long-term variability in 11 (55%) and an isolated, abrupt-onset fetal heart rate deceleration in 13 (65%). In ten (50%), loss of variability coexisted with periodic fetal heart rate decelerations. These fetal heart rate changes were significantly more prevalent in the malformed group than in a control population. There was also a significantly increased incidence of fetal distress in labor and in the requirement for primary cesarean section delivery. Perinatal mortality was 75%, reflecting the lethal nature of the malformations. Loss of long-term fetal heart rate variability associated with isolated, abrupt occurrence of fetal heart rate deceleration should raise the possibility of congenital malformations in an apparently normal pregnancy.

Abnormalities, Multiple↗
PubMed1983

Effect of dexamethasone on serum transcobalamin II concentration in women undergoing pelvic surgery.

Dexamethasone (120 mg/day for 2 days) was administered intramuscularly to young females undergoing pelvic surgery for infertility. The therapy was shown to cause a transient increase in the serum level of the granulocyte-produced transcobalamins (TCI and TCIII) and more significantly in the monocyte-produced transcobalamin (TCII), from 1220 +/- 70 to 1980 +/- 158 pg/ml (P less than 0.001). The change closely followed a transient elevation of EA rosette forming cells in the peripheral blood (from 20 to 50% of the total mononuclear cell population), which are mainly monocytes. The observed effects were shown to be independent of the surgical trauma. The results indicate that the increase in serum TCII can be attributed to an effect of dexamethasone on monocytes.

Blood Cell Count↗
PubMed1983

Fetal movements and intrauterine growth retardation.

Fetal Movement (FM) rate was evaluated in cases of symmetrical and asymmetrical intrauterine growth retardation (IUGR) and was compared to the FM rate in normal pregnancy. In the 25-36th week of gestation there was a significant decrease of FM rate in both groups of IUGR which was more pronounced in the symmetrical group. Also shown, was a gradual trend of increase of the FM rate with advancing gestational age in both groups of IUGR. Cases of asymmetrical IUGR were noted, who had markedly decreased FM until cessation. In this group of IUGR decreased FM demands prompt hospitalization and fetal heart rate monitoring so that possible respiratory failure and impending fetal death can be detected.

Female↗
PubMed1983

Effects of dexamethasone on proliferation of autologous fibroblasts and on the immune profile in women undergoing pelvic surgery for infertility.

Dexamethasone (DEX) was administered postoperatively to six patients undergoing lysis of pelvic adhesions. During therapy the proportion of T cells among the peripheral blood lymphocytes sharply declined from 60% to 20%. Response to T- and B-cell mitogens decreased to 20% to 40% of the original response prior to surgery. DEX-containing serum had an enhancing effect on the growth of autologous fibroblasts derived from pelvic adhesions (50% more than the effect of pretreatment serum). Pure cortisol and DEX induced a similar enhanced growth. The enhancing ability of the patients' sera disappeared 48 hours after discontinuation of DEX, corresponding to the recovery of the lymphoid system. If there is any benefit in steroid treatment to prevent pelvic adhesion formation, it is apparently not via an inhibitory effect on fibroblast proliferation. Such treatment, however, clearly suppresses the immune response of the patients during the early postoperative course.

Cell Division↗
PubMed1983

Familial gonadal germinative failure: endocrine and human leukocyte antigen studies.

Two primary amenorrheic sisters were diagnosed as 46,XX pure gonadal dysgenesis. Their brother, a normal phenotypic and genotypic male, was azoospermic due to primary germinative failure. Parental consanguinity was observed, suggesting an autosomal recessive inheritance. This is the first reported family in which both an otherwise healthy male and two females were affected by gonadal germinative failure. Endocrine studies showed impaired gonadal function in the three affected siblings. The two females with gonadal dysgenesis and the azoospermic male shared one human leukocyte antigen haplotype; the second haplotype, however, was different. The common haplotype was also found in the oligomenorrheic sister whose gonadotropin-releasing hormone test was compatible with normal ovarian function, in the mother, and in one of her offspring who had a normal spermiogram. Hence, linkage between human leukocyte antigens and gonadal failure in this family had been excluded. The possible etiology of familial, chromosomally competent, gonadal failure is discussed.

Amenorrhea↗
PubMed1983

Pulmonary edema occurring after isoxsuprine and dexamethasone treatment for preterm labor: Case report.

A case of maternal pulmonary edema occurring in a patient in the 32nd week of gestation is presented. This was our first case of pulmonary edema seen during a period of five years' usage of isoxsuprine in the treatment of premature labor. The patient presented was 28 years old, gravida 2, para 1, admitted to the maternity ward with premature uterine contractions. Her past history eliminate cardiac or pulmonary disease. Isoxsuprine therapy was begun with initial dose of 0.04 mg/min. and increased to 0.32 mg/min., the total dose administered was 560 mg during 48 hours. During this period she was given in dexamethasone 24 mg. Fluid balance on the first day of the treatment was +1.7 liters and on the second day +5.2 liters. Forty-eight hours from the commencement of the treatment, the patient experienced shortness of breath and chest pain. Physical examination disclosed wet rales over both lungs, sinus tachycardia and tachypnea. Laboratory examination disclosed hypopotassemia of 3 mEq/liter, hypoxemia (PO2 of 80 torr on 0.5 FiO2 face mask) with mild hyperventilation 28 torr PCO2 with normal ph 7.43. Recognition of the early signs of pulmonary edema enable swift clinical diagnosis and steps to be taken to prevent disasterous condition due to progressive hypoxemia. The prompt treatment in this complication includes discontinuation of isoxsuprine and fluid administration, placement of the patient in an erect position, intravenous furosemid 40 mg, oxygen supplement by face mask and 25 mg of meperidine. The patient's condition dramatically improved though the lung fields became completely clear from wet rales only eight hours from the start of dyspneic attack.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗