Biomedical subjects

S Monarca

Publications and source records attributed to S Monarca.

At least 37 records · Page 2Linked to original sources

PubMed1995

[Not Available].

The quality of drinking water is known to have positive or negative influences on human health. At present, in the industrialized countries microbiological contaminants in public water supplies are under control, due to the widespread introduction of effective disinfection and potabilization systems. However, of special interest are other public health problems linked with the presence of many water components or contaminants. Since the beginning of this century, fluoride showed to have beneficial effects on dental caries and the practice of adding fluoride to drinking water has been widely followed in many countries for reduction of dental caries. More recently, a large body of scientific information indicates that various characteristics related to water hardness are correlated with incidence of cardiovascular disease. Moreover, the discovery of the presence in drinking water of inorganic and organic contaminants with mutagenic/carcinogenic properties gives rise to public health concern.

Beverages↗

PubMed1995

Studies on hepatic xenobiotic-metabolizing enzymes in rats treated with insecticide deltamethrin.

The effects of the synthetic dibromo-pyrethroid insecticide deltamethrin on some hepatic phase I and II enzyme activities were studied in rat liver. The animals were treated with daily doses of 5 and 10 mg/kg of both pure insecticide or its commercial formulation (Decis), administered i.p. in corn oil for 7 days. The following enzyme activities were studied: NADPH-cytochrome-P450 reductase, aryl-hydrocarbon hydroxylase, aminopyrine N-demethylase, glutamyl cysteine synthetase, glutathione S-transferase, glutathione peroxidase, peroxisomal acyl-CoA oxidase, catalase, and urate oxidase. Both deltamethrin and its commercial formulation were effective in modifying the activities of several of these hepatic xenobiotic-metabolizing enzymes. However, some differences in enzyme modifications were found between treatment with pure or commercial deltamethrin, the latter being more active. This effect could be ascribed to additives, solvents, and chemical intermediates present in the Decis formulation. These results suggest that exposure to this deltamethrin commercial formulation could be more dangerous than exposure to deltamethrin alone, both in terms of its hepatotoxicity and/or alterations in the hepatic biotransformation of other occupational/environmental xenobiotics.

Aminopyrine N-Demethylase↗

PubMed1994

Studies of migration of potentially genotoxic compounds into water stored in pet bottles.

Coloured polyethylene terephthalate (PET) bottles for carbonated beverages were studied for potential migration of genotoxic compounds. A combined approach, using physicochemical methods and a bacterial short-term mutagenicity test (Ames test) was followed. Standard EEC and US FDA tests for total migration of non-volatile migrant compounds into distilled water were performed, together with modified tests, where freeze-drying instead of evaporation of water was used, in order to measure both volatile and non-volatile chemicals. Gas chromatography-mass spectrometry (GC-MS) analysis was performed on these residues. PET bottles filled with naturally carbonated mineral water were also used for long-term total organic carbon (TOC) and mutagenicity migration studies (up to 6 months' storage). Total migration results for PET bottles were within the EEC and US FDA limits. The use of freeze-drying for the elimination of water enabled much higher total migration data (higher than the limits) to be revealed. Some potentially genotoxic compounds (acetaldehyde, dimethyl terephthalate, terephthalic acid) were identified in these migrant compounds by GC-MS analysis. The tests for TOC migration gave a maximum value after 2 wk storage and the mutagenicity tests on non-volatile migrant compounds gave always negative results.

Carbon↗

PubMed1994

Smoking among high school students in 10 Italian towns: patterns and covariates.

The associations between tobacco smoking and demographic, socioeconomic, environmental, and behavioral factors among adolescents were investigated by administering an anonymous questionnaire to 5,221 ninth (aged 14-15 years) and 4,154 thirteenth grade (aged 18-19 years) students in 10 Italian towns. Using logistic regression analysis, both current smoking and experimental smoking were statistically associated with sibling, best friend, and partner smoking, alcohol drinking, and frequency of drunkenness in both grades and sexes. Attitude of parents toward children's smoking was also found to be associated with the probability of being a current smoker, especially among ninth graders. Parental smoking was associated with current smoking among females only. Socioeconomic factors and level of knowledge of the health consequences of smoking were not associated with either experimental or regular smoking. In conclusion, this survey suggests that social environment plays a fundamental role in both first experimentation with smoking and the process of becoming a regular smoker among adolescents. Students with one or more siblings who smoke, and especially those with best friends and a partner who smoke, were much more likely to have tried smoking and to be current smokers than students without smokers in their environment.

Adolescent↗

PubMed1994

Influence of the herbicide Linuron on growth rate and gap-junctional intercellular communication of cultured endothelial cells.

On the basis of in vivo toxicological long-term tests performed on rodents, the herbicide Linuron [3-(3,4-dichlorophenyl)-1-methoxy-1-methylurea] has been classified by the U.S. Environmental Protection Agency (EPA) (1988) as a class C carcinogen (possibly carcinogenic to humans). However, when Linuron was analyzed for genotoxicity, negative results were obtained. An epigenetic, tumor-promoting potential was hence suspected to be responsible for the oncogenic activity of the molecule. In the absence of in vivo data concerning the tumor-promoting activity of the herbicide and being well established that tumor promoters interfere with the cell growth rate and gap-junctional permeability, the effects of technical grade Linuron and of its trade formulation (Linuron 50) on these parameters were investigated. This is especially important in the case of the formulated preparation for a correct estimate of the health hazard to humans. Cytotoxicity and gap-junctional intercellular communication (GJIC) assays were performed on the endothelial cell line F-BAE GM 7373, an in vitro cell system known to be responsive to the biological effects of tumor promoters. A time- and dose-related cytotoxic effect was found for both Linuron and Linuron 50, the latter being the far more cytotoxic of the two. However, when tested at noncytotoxic concentrations over a period of 48 h, neither Linuron nor Linuron 50 altered the capacity of F-BAE GM 7373 cells to intercommunicate. On the basis of the results obtained, the possibility that Linuron and Linuron 50 are endowed with tumor-promoting activity is discussed. In vivo studies on tumor-promoting and genotoxic activity are in progress to complement the information available on the toxicological properties of Linuron and its trade preparation.

Cell Communication↗

PubMed1994

In vivo studies on enzymatic induction activity of Linuron.

The effect of the ureic herbicide Linuron [3-(3,4-dichlorophenyl)-1-methoxy-1-methylurea] on the levels of some hepatic xenobiotic metabolizing enzymes was studied in rats. The cytochrome P450-dependent monooxigenase activities of aryl hydrocarbon hydroxylase (AHH) and of aminopyrine N-demethylase (APD) were measured in rat livers after a 14-d treatment by gavage with Linuron. AHH was employed as a marker of the catalytic activity of P450IA1 and APD as a marker of the catalytic activity of P450IIB1/2. Furthermore, the enzymatic activities of the cytosolic via glutathione detoxifying enzymes glutathione peroxidase and glutathione S-transferase were assessed. Three doses of Linuron (both as pure compound and as commercial preparation) were tested. The doses tested were 150, 300, and 450 mg/kg body weight for the pure compound and 315.8, 631.6, and 947.4 mg/kg for the commercial preparation. Differences were found in the relative liver weight only in rats treated with the commercial formulation. The aryl hydrocarbon hydroxylase activity was increased with all the tested doses of pure and commercial Linuron. A reduction in the aminopyrine N-demethylase activity was noted for the highest dose of pure Linuron, whereas an increment in this activity was observed for all the doses of the commercial preparation tested. The activity of glutathione peroxidase was not affected by treatment with the pure product; however, an increment in activity was observed at all the tested doses of the commercial preparation. The glutathione S-transferase activity was reduced in both cases.

Aminopyrine N-Demethylase↗

PubMed1994

Alcohol consumption among high school students and young athletes in north Italy.

Alcohol consumption was assessed among 366 male high school students and 330 young men playing in sports teams. On the average, the athletes drank less wine, beer and spirits than the students and reported fewer drunkenness episodes. The association of alcohol drinking with sporting activity and with some independent variables was estimated by logistic regression techniques, fitting models for ordinal or nominal response variables. Point estimates of the odds ratios and their confidence limits showed: 1) an inverse relationship between alcohol drinking and sporting activity, especially as regards the consumption of spirits; 2) a strong positive relationship of alcohol drinking with both experimental and regular smoking; 3) peer alcohol drinking (best friend's and girlfriend's drinking) was the strongest predictor of the participant's alcohol consumption.

Adolescent↗

PubMed1993

Alcohol drinking among adolescents from town and mountainous areas in north Italy.

Alcohol consumption was investigated among 9th and 13th grade high school students in the town of Brescia (an urban area) and in the Local Health Unit of Breno, a mountainous area in the province of Brescia. A total of 1364 students from Brescia and 1373 from Breno completed an anonymous questionnaire during school time. In both groups alcohol consumption was greater among 13th graders (modal age: 18 years) and males than 9th graders (modal age: 14 years) and females. Beer was the favourite alcoholic beverage, but wine was the most widely consumed daily beverage: among males, about 8% in grade 9 and 15% in grade 13 drank at least one glass of wine every day. The majority of both 9th and 13th graders had more than one type of alcoholic beverage a week, although many females drank beer only. Total alcohol consumption was higher among the mountainous area students than urban students, especially 9th graders, and greater differences were observed in the consumption of beer with respect to wine. The proportion of male students from Brescia and Breno who consumed more than 6 alcohol units a week were, respectively, 17% and 25% in grade 9 and 36% and 42% in grade 13. A higher prevalence of drunkenness was found among the students from the mountainous area with respect to the urban students, especially males. Among 13th grade males, 42% of students from Brescia and 60% from Breno claimed one or more episodes of drunkenness during the year prior to the interview.

Adolescent↗

PubMed1993

Monitoring nitrite, N-nitrosodiethanolamine, and mutagenicity in cutting fluids used in the metal industry.

We carried out an integrated environmental/biological monitoring program to evaluate cancer hazards among metal industry workers exposed to cutting fluids. Several cutting fluids were sampled according to response to a semiquantitative nitrite rapid test in metal factories in central Italy. The nitrite-positive samples were analyzed for nitrite and nitrosodiethanolamine (NDELA) content and mutagenic activity. The nitrite-negative samples were analyzed only for mutagenicity. Of the total samples, 20.6% were nitrite positive, and all contained NDELA. However, nitrite content was not quantitatively predictive of the NDELA content, which varied enormously among samples (0.3-1900 mg/kg). Nitrite-negative samples were always nonmutagenic. Mutagenicity was found in half the NDELA-containing samples but was not related to nitrite or NDELA content. Nitrite screening of cutting fluids in the field is an interesting method for identifying samples that potentially contain NDELA and other unknown mutagens and, when performed with short-term mutagenicity tests, nitrite screening seems to be a valid tool by which industrial managers and health officers could minimize the health hazards associated with occupational exposure to cutting fluids.

Diethylnitrosamine↗

PubMed1992

[Factors associated with alcohol consumption in the youth of the province of Brescia].

We investigated factors related to adolescent alcohol consumption by administering an anonymous questionnaire to 2737 9th and 13th grade high-school students in the province of Brescia. A total of 1364 students coming from an urban area (the town of Brescia with about 200,000 inhabitants) and 1373 students from a mountainous area (Breno, Local Health Unit N. 37 in the Lombardy Region with 83,000 inhabitants) were interviewed. The associations between alcohol drinking and some demographic, socio-economic, behavioural and environmental variables were assessed by computing the correspective odds ratios (OR) by fitting logistic regression models for ordinal response variables ("cumulative odds" models). The results showed that the following variables were positively associated with alcohol consumption: 1) residence in a mountainous area, especially as regards spirits consumption; 2) experimental and regular smoking; 3) peer alcohol drinking (best friend and partner). Parents' daily alcohol consumption was associated with wine drinking, but not with either spirits consumption or frequency of drunkenness among students. A slight inverse relationship was also found between students' alcohol intake and their knowledge of the health hazards of alcohol, but it was not significant for all groups of students.

Adolescent↗

PubMed1991

In vitro genotoxicity of dimethyl terephthalate.

Dimethyl terephthalate (DMTP), the para configuration of dimethyl phthalate, is one of the basic monomers used in the synthesis of polyethylene terephthalate (PET) plastics. Human exposure to DMTP may primarily occur during the manufacture of PET fibers and films. The mutagenic potential of dimethyl terephthalate was evaluated using a battery of in vitro short-term tests: the Ames test; DNA single-strand break assays in CO60 cells and in primary rat hepatocytes; UDS in HeLa cells; chromosome aberration and micronucleus assays in human peripheral blood lymphocytes; selective DNA amplification in CO60 and in Syrian hamster embryo cells. The results of this battery of in vitro assays clearly show that DMTP is nongenotoxic. By contrast, other authors have found DMTP to be an in vivo clastogenic compound and suggested that the mechanisms involved in these in vivo effects seem to have nothing in common with genotoxicity and are still unknown.

Adult↗

PubMed1991

Factors associated with non-participation of women in a breast cancer screening programme in a town in northern Italy.

STUDY OBJECTIVE: The aim was to investigate the reasons for the high percentage of women refusing to attend a breast cancer screening programme in the Health District of Brescia, Italy. DESIGN: This was a survey of a sample of non-attenders to the programme, who were interviewed using a structured questionnaire. SETTING: Non-attenders all lived in a central area of the town near the screening centre. PARTICIPANTS: Of the 612 non-attenders eligible for interview, 183 could not be interviewed: one had died, 86 were away from home at two different visits, 32 were no longer resident at the known address, eight had serious health problems, 17 had undergone mastectomy, and 39 refused the interview. Overall, a total of 429 of the 612 eligible women were interviewed (70.1%). MEASUREMENTS AND MAIN RESULTS: Attenders and non-attenders were compared with respect to demographic and socioeconomic factors, use of preventive medicine, and prevalence of risk factors for breast cancer. The response was higher among less educated women, married and widowed women, and those born in the province than among more educated, single or divorced, and immigrant women. Most of the women interviewed gave practical reasons for non-participation, but lack of interest/distrust and fear/worry/anxiety also seemed important. The number of non-attenders who had had a Papanicolaou test within the previous three years was three times higher than those who had had mammography, suggesting that non-attenders were more interested in types of preventive medicine other than screening for breast cancer by mammography. Attenders and non-attenders appeared similar as regards distribution of conventional breast cancer risk factors. CONCLUSIONS: Greater effort in the information campaign might increase the participation rate in screening for breast cancer, although to a lesser extent than expected: if non-attenders potentially recruitable in our screening were added to attenders, overall compliance of the programme was about 75%, lower than that observed in some programmes in northern Europe.

Breast Neoplasms↗

PubMed1991

Drinking habits among high school students in Perugia, Italy, in 1981 and 1988: time trends and correlates.

The prevalence of alcohol consumption among 9th (14-15 years old) and 13th grade (18-19 years old) high school students in Perugia, Italy, was investigated in 1981 and 1988. Wine was the most popular beverage, although its use declined in the 7-year period. Beer consumption remained stable, but it was at a lower level than wine. Overall, alcohol consumption showed a slight decline among both 9th and 13th graders. However, the proportion of excessive drinkers increased, and a growing phenomenon of drunkenness among students was observed. Alcohol consumption was positively associated with sex and smoking and negatively associated with father's education and knowledge of the health hazards of alcohol abuse by multivariate analysis.

Adolescent↗

PubMed1991

[Cigarette smoking among high school students in Brescia: analysis of relations between smoking habit and knowledge, attitudes socioeconomic and behavioral factors].

A sample of high school students in grade 9 and 13 (14-15 and 18-19 years old respectively) in Brescia, North Italy, were interviewed to assess the relationship of smoking habit with attitudes, knowledge, behavioural and socio-environmental factors among adolescents. The associations between smoking habit, considered as a dichotomous response variable, and the other variables were assessed by estimating the prevalence ratios. The following variables were found to be associated with the students' smoking: best friend and/or partner smoking, sibling smoking, alcohol drinking and the students' judgement of the health risks of smoking. Although no conclusion can be drawn on the causal relationship of these associations due to the cross-sectional design of the survey, these findings suggest that social environment influences adolescents' smoking more than family life does. Furthermore, the knowledge of the health risks of smoking was not associated to adolescents' smoking.

Adolescent↗

PubMed1990

Urine mutagenicity and biochemical parameters as markers of exposure to petroleum pitch using a rat model.

A petroleum pitch sample collected in a carbon electrode factory was studied using a series of in vivo assays for genotoxicity and enzymatic induction capability. Rats were treated with the petroleum derivative in three doses: 100, 50, and 10 mg/kg body weight. The treatment produced a rapid excretion of mutagenic substances in the urines of the first 24 hr only in rats treated with high doses (100 and 50 mg/kg). No faecal mutagenic activity was observed. Analyses of urinary thioethers showed that urinary metabolites derived from the compounds present in the pitch-sample at the lowest dose-administered (10 mg/kg) were eliminated primarily as cysteine conjugates. The pitch sample was found to be a good inducer of pulmonary and hepatic aryl hydrocarbon hydroxylase, especially after a 50 mg/kg dose. Urinary D-glucaric acid content was always statistically increased in treated animals compared with controls, confirming the enzymatic induction activity. Hepatic glutathione-S-transferase activity increased following treatment with 50 and 10 mg/kg doses.

Animals↗

PubMed1990

Leaching of mutagens into mineral water from polyethyleneterephthalate bottles.

Polyethyleneterephthalate (PET) was tested as a source of mutagen contamination from bottles used for beverage packaging. PET bottles were filled with mineral water and stored in daylight and in the dark for different periods of time. The water samples were concentrated and the concentrates (non-volatile compounds) tested for mutagenicity with the Ames test (static tests). Total organic carbon (TOC) leaching was determined concurrently. Leaching of mutagens was also studied using dynamic tests; shaking distilled water in PET bottles. New methods were also used to test the leaching potential of both volatile and non-volatile compounds: directly testing the mutagenicity in unconcentrated water stored in PET bottles and growing Salmonella strains directly in the plastic bottles. The results were positive only for the static test, which identified leaching of mutagens after 1 month of storage in PET bottles. This activity was higher after storage in daylight.

Food Contamination↗

PubMed1989

Thioethers, mutagens, and D-glucaric acid in urine of operating room personnel exposed to anesthetics.

Mutagenic hazards related to occupational exposure to nitrous oxide and enflurane was studied in the personnel of five operating rooms using a coupled environmental and biological monitoring approach. The environmental monitoring revealed air concentrations of the two anesthetics exceedings the TLVs by 10-15-fold. These values were correlated individually with the concentrations of the two anesthetics in the expired air of the exposed subjects. The biological monitoring was carried out by determining two parameters associated with mutagen exposure (urinary mutagenicity and thioethers) and a parameter associated with the enzymatic induction (D-glucaric acid) in the urine of exposed and unexposed subjects (N = 64 and N = 37, respectively). The results showed no difference in the two groups for urine mutagenicity and D-glucaric acid, but urine thioethers were significantly increased among highly exposed subjects.

Adult↗

PubMed1989

Urinary excretion of mutagens, thioethers and D-glucaric acid in workers exposed to bitumen fumes.

The authors carried out biological monitoring of the mutagenic/carcinogenic hazards associated with exposure to bitumen fumes during paving operations, analysing some biological parameters in the urine of a group of exposed workers. The urine samples were studied for mutagenicity by the Ames test and for thioethers concentration. D-Glucaric acid urine excretion was also determined to investigate the enzymatic induction potential of bitumens. Even though, in a previous environmental monitoring phase, a low content of mutagenic/carcinogenic compounds was found in bitumen and air samples, urinary mutagenicity data of exposed workers were statistically higher than those of a group of unexposed subjects. The urinary mutagenicity increased further if exposure to bitumens was associated with cigarette smoking. Thioethers were higher only in subjects exposed simultaneously to bitumens and cigarettes. D-Glucaric acid excretion did not increase significantly. The authors think that this type of coupled environmental and biological monitoring is a valid tool for a better evaluation of the mutagenic/carcinogenic exposure to bitumens or similar complex mixtures.

Air Pollutants, Occupational↗