Biomedical subjects

S Mizuno

Publications and source records attributed to S Mizuno.

833 records · Page 47Linked to original sources

PubMedDate not supplied

C-MOS flat-panel sensor for real-time X-ray imaging.

PURPOSE: The aim of this report is to introduce a flat-panel, self-scanning, solid-state diagnostic X-ray imaging device using complementary metal-oxide-semiconductor (C-MOS) arrays for real time digital X-ray imaging and to present findings on its performance. METHODS: A device with a 5.12 cm by 5.12 cm sensor area was developed and tested. The device consists of a cesium iodide scintillator and C-MOS detector arrays. The detector arrays are composed of a regular arrangement of pixels (256x256), each of which is made of a C-MOS photodiode sensor coupled to a C-MOS FET (field effect transistor). RESULTS: A sampling speed of 30 frames per second and spatial resolution of 2.5 lines per mm were achieved. Noise level and maximum signal were 1.5 mV rms and 1.8 V, respectively. CONCLUSIONS: The C-MOS flat-panel sensor is a significant step in actualizing real-time X-ray imaging. A large area sensor needs to be fabricated for clincial use.

Cesium↗

PubMedDate not supplied

A high density culture of hepatocytes using a reticulated polyvinyl formol resin.

To enable high density culture of hepatocytes to be used as a hybrid artificial liver support or bioreactor system, collagen coated reticulated polyvinyl formal (PVF) resin (a filter material with a porosity of more than 80%) was used for the primary culture of hepatocytes. Stationary and perfusion culture experiments using PVF resin and monolayer culture were performed as control experiments. Due to the porous structure of the substrate material, hepatocytes were able to penetrate the reticulated pores of the PVF resin, and adhere to and spread on its surface. The densities of hepatocytes attained with PVF were about 10 times as high as those in the monolayer culture using conventional collagen coated Petri dishes. Hepatocytes immobilized in the PVF resin showed viability, as assessed by glutamic pyruvic transaminase (GPT) activity in the medium. Perfusion culture with PVF resin showed stable, high level metabolism, ammonium removal, and urea secretion comparable to the monolayer culture. It is concluded that perfusion culture with PVF resin is useful in attaining a high density culture of hepatocytes.

Animals↗

PubMedDate not supplied

Aging factors and cardiovascular dimensions: a longitudinal study.

The effects of selected aging factors including body weight, blood pressure, and radiation dose on the cardiac and aortic arch transverse diameters, based on posteroanterior and lateral radiographs obtained 17 to 22 years apart were evaluated for 200 normal subjects. Increases in body weight and blood pressure caused increases in transverse cardiac diameter of 26% and 2%, respectively, but no appreciable effect on aortic arch transverse diameter. Radiation dose caused no significant effects. Effects of increases in body weight and blood pressure on cardiac and aortic arch transverse diameters with age ought not be overestimated in healthy adults.

Adult↗

PubMedDate not supplied

Evaluation of protein kinase inhibitors in an assay system containing multiple protein kinase activities.

Specificities of several known protein kinase inhibitors were evaluated using an assay system in which activities of protein kinase A, protein kinase C, protein tyrosine kinase and calmodulin-dependent protein kinase III were simultaneously detected. Inhibitory spectra of H-89, K252a, H-7, staurosporine, tyrphostin and herbimycin A observed in the assay system were similar to those reported in the purified protein kinase assay systems. It was also found that KN-62 selectively inhibited calmodulin-dependent protein kinase III activity. These data suggest that the assay system may be effective and practicable in evaluating specificities and screening of new protein kinase inhibitors.

1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine↗

PubMedDate not supplied

Diffuse glomerulosclerosis without tubular injury does not directly manifest renal dysfunction in nephrotic mice (ICGN strain).

Chronic renal disease is characterized by pathological conditions, caused by a progressive and irreversible loss of renal function. The nephrotic mouse (ICGN strain) is a useful model for demonstrating progressive nephrotic syndrome (NS). In the present study, we examined the natural course of kidney disease shown by the NS mice, aging 6-26 weeks, in order to characterize their pathological conditions. Urine and serum biochemistry revealed that massive albuminuria and hypoalbuminemia were evident in the NS mice throughout the observation period and that blood urea nitrogen (BUN) and serum creatinine levels began to increase from 14 weeks after birth. Renal histopathology demonstrated that glomerulosclerotic lesion was diffusely observed in the renal cortical area from 6 weeks after birth. Furthermore, the tubular lesions, characterized by tubular cellular atrophy and luminal cast formation, became mild to moderate and severe in the 14- and 26-week-old NS mice, respectively. Although the 6- and 10-week-old NS mice exhibited diffuse mesangial expansion, BUN and serum creatinine levels were normal as long as the tubular architecture remained almost intact. There was a good correlation between BUN level and tubular injury index (r2 = 0.860, p < 0.001), while no significant relationship was seen between the BUN level and glomerular matrix index. Our model, an experimental analogue of human chronic renal disease, clearly demonstrates that glomerulosclerosis is not always a direct determinant for renal dysfunction.

Actins↗